Abstract:
:The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industrial interest. Among the PKSI domains, the ketosynthase domain (KS) was used to screen a large soil metagenomic library containing more than 100,000 clones to detect those containing PKS genes. Over 60,000 clones were screened, and 139 clones containing KS domains were detected. A 700-bp fragment of the KS domain was sequenced for 40 of 139 randomly chosen clones. None of the 40 protein sequences were identical to those found in public databases, and nucleic sequences were not redundant. Phylogenetic analyses were performed on the protein sequences of three metagenomic clones to select the clones which one can predict to produce new compounds. Two PKS-positive clones do not belong to any of the 23 published PKSI included in the analysis, encouraging further analyses on these two clones identified by the selection process.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Ginolhac A,Jarrin C,Gillet B,Robe P,Pujic P,Tuphile K,Bertrand H,Vogel TM,Perrière G,Simonet P,Nalin Rdoi
10.1128/AEM.70.9.5522-5527.2004keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
5522-7issue
9eissn
0099-2240issn
1098-5336pii
70/9/5522journal_volume
70pub_type
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