Characterization of Pseudomonas aeruginosa bacteriophage UNL-1, a bacterial virus with a novel UV-A-inducible DNA damage reactivation phenotype.

Abstract:

:UNL-1, a lytic virus of Pseudomonas aeruginosa, was observed to express a novel inducible DNA damage reactivation activity in UV-A-irradiated P. aeruginosa host cells. The expression of bacteriophage reactivation was quantified in hosts exposed to either UV-C or UV-A radiation. While reactivation of UV-C-damaged UNL-1 was not inducible in UV-C-irradiated host cells, an approximately 13-fold induction was observed in UV-A-irradiated host cells. When host cells were exposed to sunlight, reactivation of damaged UNL-1 virus increased eightfold. The UV-A induction of UNL-1 DNA damage reactivation was supported in hosts lacking recA gene function. This report is the first description of a recA-independent, UV-inducible virus DNA damage repair system. Our findings suggest that a combination of both host and virus DNA repair processes contribute to the persistence and sustained replication of some bacterial viruses in aquatic environments.

journal_name

Appl Environ Microbiol

authors

Shaffer JJ,Jacobsen LM,Schrader JO,Lee KW,Martin EL,Kokjohn TA

doi

10.1128/AEM.65.6.2606-2613.1999

keywords:

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

2606-13

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

65

pub_type

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