Reengineering Escherichia coli for Succinate Production in Mineral Salts Medium.

abstract：:Rapid population growth and urban development along waterways and coastal areas have led to decreasing water quality. To examine the effects of upstream anthropogenic activities on microbiological water quality, methods for source-specific testing are required. In this study, molecular assays targeting human enterovir...

journal_title：Applied and environmental microbiology

authors： Fong TT,Griffin DW,Lipp EK

更新日期：2005-04-01 00:00:00

abstract：:This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

journal_title：Applied and environmental microbiology

authors： Hogg JC,Lehane MJ

更新日期：1999-09-01 00:00:00

abstract：:We characterized the intracellular symbiotic microbiota of the bamboo pseudococcid Antonina crawii by performing a molecular phylogenetic analysis in combination with in situ hybridization. Almost the entire length of the bacterial 16S rRNA gene was amplified and cloned from A. crawii whole DNA. Restriction fragment l...

journal_title：Applied and environmental microbiology

authors： Fukatsu T,Nikoh N

更新日期：2000-02-01 00:00:00

abstract：:The presence of bacteria in salted anchovies during and at the end of the curing process was investigated. Attempts to isolate bacteria under aerobic or anaerobic conditions led to the isolation of only bacteria of the genus Pediococcus which were identified as Pediococcus halophilus. The isolates correspond to a rath...

journal_title：Applied and environmental microbiology

authors： Villar M,de Ruiz Holgado AP,Sanchez JJ,Trucco RE,Oliver G

更新日期：1985-03-01 00:00:00

abstract：:1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been consider...

journal_title：Applied and environmental microbiology

authors： Monincová M,Prokop Z,Vévodová J,Nagata Y,Damborsky J

更新日期：2007-03-01 00:00:00

abstract：:The first inducible Arthrobacter overexpression system, based on the promoter/operator and the repressor of the 6-D-hydroxynicotine oxidase gene of Arthrobacter nicotinovorans, is described here. Nicotine-dependent overproduction and affinity purification of recombinant proteins are presented. The system will allow th...

journal_title：Applied and environmental microbiology

authors： Sandu C,Chiribau CB,Sachelaru P,Brandsch R

更新日期：2005-12-01 00:00:00

abstract：:Nitrite-dependent anaerobic oxidation of methane (n-damo) and ammonium (anammox) are two recently discovered processes in the nitrogen cycle that are catalyzed by n-damo bacteria, including "Candidatus Methylomirabilis oxyfera," and anammox bacteria, respectively. The feasibility of coculturing anammox and n-damo bact...

journal_title：Applied and environmental microbiology

authors： Luesken FA,Sánchez J,van Alen TA,Sanabria J,Op den Camp HJ,Jetten MS,Kartal B

更新日期：2011-10-01 00:00:00

abstract：:The degradation of p-nitrophenol (PNP) by Moraxella and Pseudomonas spp. involves an initial monooxygenase-catalyzed removal of the nitro group. The resultant hydroquinone is subject to ring fission catalyzed by a dioxygenase enzyme. We have isolated a strain of an Arthrobacter sp., JS443, capable of degrading PNP wit...

journal_title：Applied and environmental microbiology

authors： Jain RK,Dreisbach JH,Spain JC

更新日期：1994-08-01 00:00:00

abstract：:The well-established killing of bacteria by copper surfaces, also called contact killing, is currently believed to be a combined effect of bacterial contact with the copper surface and the dissolution of copper, resulting in lethal bacterial damage. Iron can similarly be released in ionic form from iron surfaces and w...

journal_title：Applied and environmental microbiology

authors： Mathews S,Kumar R,Solioz M

更新日期：2015-09-01 00:00:00

abstract：:The strain Burkholderia cepacia G4 aerobically mineralized trichloroethene (TCE) to CO(2) over a time period of approximately 20 h. Three biodegradation experiments were conducted with different bacterial optical densities at 540 nm (OD(540)s) in order to test whether isotope fractionation was consistent. The resultin...

journal_title：Applied and environmental microbiology

authors： Barth JA,Slater G,Schüth C,Bill M,Downey A,Larkin M,Kalin RM

更新日期：2002-04-01 00:00:00

abstract：:Low and high levels of resistance to the benzimidazole fungicides benomyl and thiophanate-methyl were observed in field isolates of Monilinia fructicola, which is the causative agent of brown rot of stone fruit. Isolates that had low levels of resistance (hereafter referred to as LR isolates) and high levels of resist...

journal_title：Applied and environmental microbiology

authors： Ma Z,Yoshimura MA,Michailides TJ

更新日期：2003-12-01 00:00:00

abstract：:In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

journal_title：Applied and environmental microbiology

authors： Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:A novel β-(1,3)-glucanase gene designated lamC, cloned from Corallococcus sp. strain EGB, contains a fascin-like module and a glycoside hydrolase family 16 (GH16) catalytic module. LamC displays broad hydrolytic activity toward various polysaccharides. Analysis of the hydrolytic products revealed that LamC is an exo-a...

journal_title：Applied and environmental microbiology

authors： Zhou J,Li Z,Wu J,Li L,Li D,Ye X,Luo X,Huang Y,Cui Z,Cao H

更新日期：2017-08-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis strain E40 filaments were developed under conditions of a reduced water activity (a(w)) of 0.95 in tryptic soy broth (TSB) or tryptic soy agar (TSA) supplemented with 8% or 7% NaCl, respectively. Filament formation was accompanied by an increase of biomass without an increase in...

journal_title：Applied and environmental microbiology

authors： Stackhouse RR,Faith NG,Kaspar CW,Czuprynski CJ,Wong AC

更新日期：2012-04-01 00:00:00

abstract：:Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains are closely related human pathogens that are responsible for food-borne epidemics in many countries. Integration host factor (IHF) and the locus of enterocyte effacement-encoded regulator (Ler) are needed for the expression o...

journal_title：Applied and environmental microbiology

authors： Li M,Rosenshine I,Tung SL,Wang XH,Friedberg D,Hew CL,Leung KY

更新日期：2004-09-01 00:00:00

abstract：:[This corrects the article on p. 1580 in vol. 58.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1992-08-01 00:00:00

abstract：:We developed a new cell surface display system in Pseudomonas putida KT2442 using OprF, an outer membrane protein of Pseudomonas aeruginosa, as an anchoring motif in a C-terminal deletion-fusion strategy. The Pseudomonas fluorescens SIK W1 lipase gene was fused to two different C-terminal truncated OprF genes, and the...

journal_title：Applied and environmental microbiology

authors： Lee SH,Lee SY,Park BC

更新日期：2005-12-01 00:00:00

abstract：:The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -inde...

journal_title：Applied and environmental microbiology

authors： Chouaia B,Rossi P,Montagna M,Ricci I,Crotti E,Damiani C,Epis S,Faye I,Sagnon N,Alma A,Favia G,Daffonchio D,Bandi C

更新日期：2010-11-01 00:00:00

abstract：:Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitat...

journal_title：Applied and environmental microbiology

authors： Hammes F,Boon N,Vital M,Ross P,Magic-Knezev A,Dignum M

更新日期：2011-02-01 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00

abstract：:The relationship between physiochemical surface parameters and adhesion of bacterial cells to negatively charged polystyrene was studied. Cell surface hydrophobicity and electrokinetic potential were determined by contact angle measurement and electrophoresis, respectively. Both parameters influence cell adhesion. The...

journal_title：Applied and environmental microbiology

authors： van Loosdrecht MC,Lyklema J,Norde W,Schraa G,Zehnder AJ

更新日期：1987-08-01 00:00:00

abstract：:Sourdough lactic acid bacteria were preliminarily screened for proteolytic activity by using a digest of albumin and globulin polypeptides as a substrate. Based on their hydrolysis profile patterns, Lactobacillus alimentarius 15M, Lactobacillus brevis 14G, Lactobacillus sanfranciscensis 7A, and Lactobacillus hilgardii...

journal_title：Applied and environmental microbiology

authors： Di Cagno R,De Angelis M,Lavermicocca P,De Vincenzi M,Giovannini C,Faccia M,Gobbetti M

更新日期：2002-02-01 00:00:00

abstract：:The bacterial content of specially treated cottons used by other investigators to test human pulmonary responses to cotton dust was examined. Cotton from Lubbock, Tex. and Stoneville, Miss. were either (i) harvested by machine and handled as commercial bale cotton, (ii) harvested as closed bolls with bracts intact and...

journal_title：Applied and environmental microbiology

authors： Millner PD,Ericson KE,Marsh PB

更新日期：1982-08-01 00:00:00

abstract：:Many pathogenic yeast species are asexual and therefore not involved in intra- or interspecies mating. However, high-frequency transfer of plasmid DNA was observed when pathogenic and food-borne yeasts were grown together. This property could play a crucial role in the spread of virulence and drug resistance factors a...

journal_title：Applied and environmental microbiology

authors： Mentel M,Spírek M,Jørck-Ramberg D,Piskur J

更新日期：2006-07-01 00:00:00

abstract：:Excessive NH(3) production in the rumen is a major nutritional inefficiency in ruminant animals. Experiments were undertaken to compare the rates of NH(3) production from different substrates in ruminal fluid in vitro and to assess the role of asaccharolytic bacteria in NH(3) production. Ruminal fluid was taken from f...

journal_title：Applied and environmental microbiology

authors： Eschenlauer SC,McKain N,Walker ND,McEwan NR,Newbold CJ,Wallace RJ

更新日期：2002-10-01 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:The UV sunscreen role commonly ascribed to mycosporine-like amino acids (MAAs) was investigated with an isolate of the terrestrial cyanobacterium Gloeocapsa sp. strain C-90-Cal-G.(2), which accumulates intracellularly an MAA with absorbance maximum at 326 nm but produces no extracellular sunscreen compound (i.e., scyt...

journal_title：Applied and environmental microbiology

authors： Garcia-Pichel F,Wingard CE,Castenholz RW

更新日期：1993-01-01 00:00:00

abstract：:During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...

journal_title：Applied and environmental microbiology

authors： Crépin L,Truong NM,Bloem A,Sanchez I,Dequin S,Camarasa C

更新日期：2017-02-15 00:00:00

abstract：:A real-time PCR-based method targeting the 18S rRNA gene was developed for the quantitative detection of Hartmannella vermiformis, a free-living amoeba which is a potential host for Legionella pneumophila in warm water systems and cooling towers. The detection specificity was validated using genomic DNA of the closely...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Valster RM,Wullings BA,Boonstra H,Smidt H,van der Kooij D

更新日期：2006-09-01 00:00:00

abstract：:The quorum sensing (QS) system controls bacterial group behaviors in response to cell density. In vibrios, LuxR and AphA are two master QS regulators (MQSRs) controlling gene expression in response to high or low cell density. Other regulators involved in the regulation of these two MQSRs and QS pathways remain to be ...

journal_title：Applied and environmental microbiology

authors： Gao X,Wang X,Mao Q,Xu R,Zhou X,Ma Y,Liu Q,Zhang Y,Wang Q

更新日期：2018-05-31 00:00:00