Molecular assays for targeting human and bovine enteric viruses in coastal waters and their application for library-independent source tracking.

abstract：:The human gastrointestinal (GI) tract provides home to a complex microbial community, collectively termed microbiota. Although major efforts have been made to describe the diversity and stability of the microbiota, functional studies have been largely restricted to intestinal isolates and include few community studies...

journal_title：Applied and environmental microbiology

authors： Booijink CC,Boekhorst J,Zoetendal EG,Smidt H,Kleerebezem M,de Vos WM

更新日期：2010-08-01 00:00:00

abstract：:A Pseudomonas putida strain (MC4) that can utilize 2,3-dichloro-1-propanol (DCP) and several aliphatic haloacids and haloalcohols as sole carbon and energy source for growth was isolated from contaminated soil. Degradation of DCP was found to start with oxidation and concomitant dehalogenation catalyzed by a 72-kDa mo...

journal_title：Applied and environmental microbiology

authors： Arif MI,Samin G,van Leeuwen JG,Oppentocht J,Janssen DB

更新日期：2012-09-01 00:00:00

abstract：:The obligate intracellular growth of Rickettsia prowazekii places severe restrictions on the analysis of rickettsial gene expression. With a small genome, predicted to code for 835 proteins, identifying which proteins are differentially expressed in rickettsiae that are isolated from different hosts or that vary in vi...

journal_title：Applied and environmental microbiology

authors： Tucker AM,Driskell LO,Pannell LK,Wood DO

更新日期：2011-07-01 00:00:00

abstract：:Few techniques are currently available for quantifying specific prokaryotic taxa in environmental samples. Quantification of specific genotypes has relied mainly on oligonucleotide hybridization to extracted rRNA or intact rRNA in whole cells. However, low abundance and cellular rRNA content limit the application of t...

journal_title：Applied and environmental microbiology

authors： Suzuki MT,Taylor LT,DeLong EF

更新日期：2000-11-01 00:00:00

abstract：:Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

journal_title：Applied and environmental microbiology

authors： García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

更新日期：2003-12-01 00:00:00

abstract：:The alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When transferred into Lactococcus lactis and Pediococcus acidilactici strains, aga modified the sugar fermentation profile of...

journal_title：Applied and environmental microbiology

authors： Boucher I,Parrot M,Gaudreau H,Champagne CP,Vadeboncoeur C,Moineau S

更新日期：2002-12-01 00:00:00

abstract：:We have engineered Escherichia coli to overproduce saturated and monounsaturated aliphatic methyl ketones in the C₁₁ to C₁₅ (diesel) range; this group of methyl ketones includes 2-undecanone and 2-tridecanone, which are of importance to the flavor and fragrance industry and also have favorable cetane numbers (as we re...

journal_title：Applied and environmental microbiology

authors： Goh EB,Baidoo EE,Keasling JD,Beller HR

更新日期：2012-01-01 00:00:00

abstract：:A total of 25 marine caulobacters were isolated from littoral marine sources. Several aspects of their physiology and morphology were examined, as well as their suitability for genetic manipulation in laboratory cultivation. Caulobacters were readily isolated from all sources, including samples from areas containing p...

journal_title：Applied and environmental microbiology

authors： Anast N,Smit J

更新日期：1988-03-01 00:00:00

abstract：:We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...

journal_title：Applied and environmental microbiology

authors： Pankowski JA,Puckett SM,Nano FE

更新日期：2016-01-15 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:In this report, we describe the selective cloning of large DNA fragments from magnetotactic metagenomes from various aquatic habitats. This was achieved by a two-step magnetic enrichment which allowed the mass collection of environmental magnetotactic bacteria (MTB) virtually free of nonmagnetic contaminants. Four fos...

journal_title：Applied and environmental microbiology

authors： Jogler C,Lin W,Meyerdierks A,Kube M,Katzmann E,Flies C,Pan Y,Amann R,Reinhardt R,Schüler D

更新日期：2009-06-01 00:00:00

abstract：:In Comamonas sp. strain JS46, 3-nitrobenzoate (3Nba) is initially oxidized at the 3,4 position by a dioxygenase, which results in release of nitrite and production of protocatechuate. The locus coding for the 3Nba dioxygenase (designated mnb, for m-nitrobenzoate) was mobilized from strain JS46 using a plasmid capture ...

journal_title：Applied and environmental microbiology

authors： Providenti MA,Shaye RE,Lynes KD,McKenna NT,O'brien JM,Rosolen S,Wyndham RC,Lambert IB

更新日期：2006-04-01 00:00:00

abstract：:Hot-water extracts of mesquite (Prosopis glandulosa) wood were assayed for their total carbohydrate, reducing sugar, and glucose content. These hydrolysates were then used as complete media for yeast growth. A total of 10 strains of yeasts were evaluated for their biomass production in the mesquite wood hydrolysates. ...

journal_title：Applied and environmental microbiology

authors： Stanlake GJ

更新日期：1986-08-01 00:00:00

abstract：:Peanut kernels (Tainan 9, a Spanish cultivar) inoculated with Aspergillus parasiticus, A. flavus, A. niger, or A. ochraceus as well as noninoculated kernels were incubated in a humidified environment (relative humidity, 100%) at 25(deg)C for 7 weeks. Internal fungal populations and changes in moisture and sucrose cont...

journal_title：Applied and environmental microbiology

authors： Chiou RY

更新日期：1997-03-01 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00

abstract：:The transport of branched-chain amino acids in Streptococcus thermophilus was energy dependent. The metabolic inhibitors of glycolysis and ATPase enzymes were active, but the proton-conducting uncouplers were not. Transport was optimal at temperatures of between 30 and 45 degrees C and at pH 7.0 for the three amino ac...

journal_title：Applied and environmental microbiology

authors： Akpemado KM,Bracquart PA

更新日期：1983-01-01 00:00:00

abstract：:Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and ...

journal_title：Applied and environmental microbiology

authors： Chu FS,Huang X,Wei RD,Carmichael WW

更新日期：1989-08-01 00:00:00

abstract：:Reporter gene technology was used to observe the regulation of the alginate biosynthesis gene, algC in a mucoid strain of Pseudomonas aeruginosa in developing and mature biofilms in continuous culture on Teflon and glass substrata. The plasmid pNZ63, carrying an algC-lacZ transcriptional fusion, was shown to not be di...

journal_title：Applied and environmental microbiology

authors： Davies DG,Geesey GG

更新日期：1995-03-01 00:00:00

abstract：:Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

journal_title：Applied and environmental microbiology

authors： DePaola A,McLeroy S,McManus G

更新日期：1997-06-01 00:00:00

abstract：:Growth inhibition by isoleucine hydroxamate in Serratia marcescens was significantly enhanced by adding valine plus leucine and by using glycerol as the carbon source. Isoleucine hydroxamate-resistant mutants were isolated under conditions in which growth inhibition was enhanced. One of the mutants, strain GIHVLr2179,...

journal_title：Applied and environmental microbiology

authors： Kisumi M,Komatsubara S,Chibata I

更新日期：1977-12-01 00:00:00

abstract：:Lactococcus lactis subsp. cremoris MG1363 is a model for the lactic acid bacteria (LAB) used in the dairy industry. The proteolytic system, consisting of a proteinase, several peptide and amino acid uptake systems, and a host of intracellular peptidases, plays a vital role in nitrogen metabolism and is of eminent impo...

journal_title：Applied and environmental microbiology

authors： Huang C,Hernandez-Valdes JA,Kuipers OP,Kok J

更新日期：2020-04-01 00:00:00

abstract：:Antigens from the methicillin-resistant Staphylococcus aureus (MRSA) cell wall have been shown to be immunogenic in vivo and upregulated during biofilm growth. In this study, we created purified, recombinant forms of selected antigens and biofilm-upregulated, cell wall-associated proteins. These proteins were shown to...

journal_title：Applied and environmental microbiology

authors： Brady RA,Leid JG,Kofonow J,Costerton JW,Shirtliff ME

更新日期：2007-10-01 00:00:00

abstract：:The nonlinear thermal inactivation of Aspergillus niger spores in phosphate-citrate buffer was studied. The thermal inactivation pattern of the spore consisted of a shoulder, an accelerated decline, and a tail at various constant temperatures around 60 degrees C. The pattern fitted a thermotolerant subpopulation model...

journal_title：Applied and environmental microbiology

authors： Fujikawa H,Itoh T

更新日期：1996-10-01 00:00:00

abstract：:Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

更新日期：1999-09-01 00:00:00

abstract：:The pathways responsible for cell wall polysaccharide biosynthesis are vital in eukaryotic microorganisms. The corresponding synthases are potential targets of inhibitors such as fungicides. Despite their fundamental and economical importance, most polysaccharide synthases are not well characterized, and their molecul...

journal_title：Applied and environmental microbiology

authors： Briolay A,Bouzenzana J,Guichardant M,Deshayes C,Sindt N,Bessueille L,Bulone V

更新日期：2009-04-01 00:00:00

abstract：:Stored sugarbeet roots began fermenting within 24 h after oxygen was depleted at 26 C when the resident bacterial populations increased dramatically. Most of the bacteria present after anaerobic storage for 7 days at 26 C could hydrolyze sucrose in vitro. Although pH and sucrose levels decreased and invert sugar level...

journal_title：Applied and environmental microbiology

authors： Cole DF,Bugbee WM

更新日期：1976-05-01 00:00:00

abstract：:Cotton flowers in replicate plots in two fields near Phoenix, Ariz., were tagged in June at the beginning of the flowering period. Flowers or developing bolls from these tagged flowers were inoculated on the involucral (bracteal) nectaries with dry spores of Aspergillus flavus. The bolls were harvested as they matured...

journal_title：Applied and environmental microbiology

authors： Klich MA,Chmielewski MA

更新日期：1985-09-01 00:00:00

abstract：:Epimerization of sugar nucleotides is central to the structural diversification of monosaccharide building blocks for cellular biosynthesis. Epimerase applicability to carbohydrate synthesis can be limited, however, by the high degree of substrate specificity exhibited by most sugar nucleotide epimerases. Here, we dis...

journal_title：Applied and environmental microbiology

authors： Rapp C,van Overtveldt S,Beerens K,Weber H,Desmet T,Nidetzky B

更新日期：2020-12-04 00:00:00

abstract：:The natural abundance of stable carbon isotopes measured in bacterial nucleic acids extracted from estuarine bacterial concentrates was used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of Pseudomonas aeruginosa and nucleic acids extracted from cultures rese...

journal_title：Applied and environmental microbiology

authors： Coffin RB,Velinsky DJ,Devereux R,Price WA,Cifuentes LA

更新日期：1990-07-01 00:00:00

abstract：:Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount...

journal_title：Applied and environmental microbiology

authors： Akeboshi H,Chiba Y,Kasahara Y,Takashiba M,Takaoka Y,Ohsawa M,Tajima Y,Kawashima I,Tsuji D,Itoh K,Sakuraba H,Jigami Y

更新日期：2007-08-01 00:00:00