Abstract:
:Cotton flowers in replicate plots in two fields near Phoenix, Ariz., were tagged in June at the beginning of the flowering period. Flowers or developing bolls from these tagged flowers were inoculated on the involucral (bracteal) nectaries with dry spores of Aspergillus flavus. The bolls were harvested as they matured in August, and the seeds were assessed for the presence of the fungus. The number of infected seed from flowers and bolls inoculated up to 25 days after anthesis was significantly higher than that in uninoculated controls. Seeds from bolls inoculated after 25 days postanthesis did not differ significantly from controls in degree of infection. We postulate that the sharp decline in the ability of the fungus to infect the plant and seed is a result of physical or biochemical changes in the boll as it reaches physiological maturity or biochemical changes in the entire plant as it develops.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Klich MA,Chmielewski MAdoi
10.1128/AEM.50.3.602-604.1985keywords:
subject
Has Abstractpub_date
1985-09-01 00:00:00pages
602-4issue
3eissn
0099-2240issn
1098-5336journal_volume
50pub_type
杂志文章abstract::Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the su...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02820-17
更新日期:2018-04-16 00:00:00
abstract::An experiment was conducted with subsurface sediments from Oak Ridge National Laboratory to determine the potential for reduction of U(VI) under sulfate-reducing conditions with either ethanol or acetate as the electron donor. The results showed extensive U(VI) reduction in sediments supplied with either electron dono...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00420-13
更新日期:2013-07-01 00:00:00
abstract::A mutant of the peptide antibiotic nisin in which the dehydroalanine residue at position 5 has been replaced by an alanine has been produced and structurally characterized. It is shown to have activity very similar to that of wild-type nisin in inhibiting growth of Lactococcus lactis and Micrococcus luteus but is very...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.8.2966-2969.1996
更新日期:1996-08-01 00:00:00
abstract::Microbial communities play important roles in the functioning of coral reef communities. However, extensive autofluorescence of coral tissues and endosymbionts limits the application of standard fluorescence in situ hybridization (FISH) techniques for the identification of the coral-associated bacterial communities. T...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.4.3016-3020.2006
更新日期:2006-04-01 00:00:00
abstract::Male weanling rats fed diets containing fermented and unfermented tannin-free sorghum meals naturally contaminated with traces of ochratoxin A, zearalenone, and an unidentified toxic substance developed anorexia. Mean changes in body weight over a 28-day feeding period for rats fed fermented and unfermented sorghum me...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.5.1118-1125.1984
更新日期:1984-05-01 00:00:00
abstract::Streptococcus mutans 6715-15 and Streptococcus sanguis 10558 were grown together in continuous culture with glucose as the limiting carbon source. The relationship of growth rate to substrate concentration was determined for pure cultures of each organism in continuous and batch cultures. A model based on competition ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.4.1277-1282.1983
更新日期:1983-04-01 00:00:00
abstract::Here, we report the genome of one gammaproteobacterial member of the gut microbiota, for which we propose the name "Candidatus Schmidhempelia bombi," that was inadvertently sequenced alongside the genome of its host, the bumble bee, Bombus impatiens. This symbiont is a member of the recently described bacterial order ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00322-14
更新日期:2014-07-01 00:00:00
abstract::Detection of pathogens in contaminated food products by PCR can result in false-positive data due to the amplification of DNA from nonviable cells. A new method based on reverse transcription-PCR (RT-PCR) amplification of mRNA for the specific detection of viable Listeria monocytogenes was developed. The expression of...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.11.4441-4448.1997
更新日期:1997-11-01 00:00:00
abstract::Four DNA probes were derived that hybridized specifically to DNA from Lactobacillus acidophilus O. The probes were constructed by randomly cloning lactobacillus DNA in plasmid vector pBR322. Two of the probes (pSR1 and pSR2) were composed of vector and plasmid DNA inserts (3.6 and 1.6 kb, respectively); the others (pS...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.11.3871-3877.1993
更新日期:1993-11-01 00:00:00
abstract::This is the first description, to our knowledge, of the distribution of human polyomavirus and simian virus 40 (SV40) in urban sewage. Using a nested-PCR procedure, we report the detection of human polyomaviruses JC virus (JCV) and BK virus (BKV) but not SV40 in a high percentage of urban sewage samples obtained from ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.1.238-245.2000
更新日期:2000-01-01 00:00:00
abstract::The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00280-20
更新日期:2020-05-05 00:00:00
abstract::Macroalgae (or seaweeds) are considered potential biomass feedstocks for the production of renewable fuels and chemicals. Their sugar composition is different from that of lignocellulosic biomasses, and in green species, including Ulva lactuca, the major sugars are l-rhamnose and d-glucose. C. beijerinckii DSM 6423 ut...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02656-18
更新日期:2019-02-20 00:00:00
abstract::A selective procedure was used to isolate pectinolytic intestinal bacteria from human subjects. The three isolates with the greatest pectinolytic activity utilized pectin and a few related compounds as fermentable substrates for growth but did not utilize any other compound tested. Thus, their substrate utilization pa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.1.172-173.1985
更新日期:1985-07-01 00:00:00
abstract::Rabbits have been increasingly linked to the persistence of paratuberculosis (Johne's disease) in domestic ruminants in the United Kingdom. The aims of this study were to determine the routes of intraspecies transmission of Mycobacterium avium subspecies paratuberculosis (MAP) in rabbits and to estimate the probabilit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.1.398-403.2006
更新日期:2006-01-01 00:00:00
abstract::The putative role of biting flies in Bartonella transmission among ruminants was investigated. Amplification of the Bartonella citrate synthase gene from 83 Hippoboscidae was detected in 94% of 48 adult Lipoptena cervi flies, 71% of 17 adult Hippobosca equina flies, 100% of 20 adult Melophagus ovinus flies, and 100% o...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.10.6302-6305.2004
更新日期:2004-10-01 00:00:00
abstract::Lipid accumulation and fatty acid composition in Candida 107 have been studied using a two-stage continuous culture system in which the first vessel was run under carbon-limited conditions and then the entire output was passed into a second vessel, where lipid accumulation was stimulated by adding only glucose. Maximu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.3.577-584.1977
更新日期:1977-03-01 00:00:00
abstract::Degradation of polycyclic aromatic hydrocarbons (PAHs) and survival of bacteria in soil was investigated by applying different inoculation protocols. The soil was inoculated with Sphingomonas paucimobilis BA 2 and strain BP 9, which are able to degrade anthracene and pyrene, respectively. CFU of soil bacteria and of t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.1.359-362.1998
更新日期:1998-01-01 00:00:00
abstract::This work demonstrates the ability of a bacterial concentration and recovery procedure combined with three different PCR assays targeting the lacZ, wecG, and 16S rRNA genes, respectively, to detect the presence of total coliforms in 100-ml samples of potable water (presence/absence test). PCR assays were first compare...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00546-14
更新日期:2014-07-01 00:00:00
abstract::Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02995-12
更新日期:2013-03-01 00:00:00
abstract::Botulinum neurotoxin (BoNT), produced by neurotoxigenic clostridia, is the most potent biological toxin known and the causative agent of the paralytic disease botulism. The nutritional, environmental, and genetic regulation of BoNT synthesis, activation, stability, and toxin complex (TC) formation is not well studied....
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00642-17
更新日期:2017-06-16 00:00:00
abstract::Pseudomonas putida aggressively colonizes root surfaces and is agglutinated by a root surface glycoprotein. Mutants of P. putida derived chemically or by Tn5 insertion demonstrated enhanced or decreased agglutinability. Two nonagglutinable Tn5 mutants (Agg) and two mutants with enhanced agglutinability (Agg) possessed...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.2.375-380.1988
更新日期:1988-02-01 00:00:00
abstract::A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.8.4618-4627.2003
更新日期:2003-08-01 00:00:00
abstract::Identification and classification of Vibrio species have relied upon band pattern methods (e.g., amplified fragment length polymorphism) and DNA-DNA hybridization. However, data generated by these methods cannot be used to build an online electronic taxonomy. In order to overcome these limitations, we developed the fi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00020-07
更新日期:2007-07-01 00:00:00
abstract::Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01486-12
更新日期:2012-10-01 00:00:00
abstract::The ultrasonic determination of microbial population was carried out by using a new apparatus composed of two piezoelectric membranes. When the apparatus was immersed in a medium containing cells and an arbitrary voltage was charged on a piezoelectric membrane, an output voltage was obtained from the other membrane. T...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.4.632-637.1981
更新日期:1981-10-01 00:00:00
abstract::Bacteriophages are very abundant in the biosphere, and viral infection is believed to affect the activity and genetic diversity of bacterial communities in aquatic environments. Lysogenic conversion, for example, can improve host fitness and lead to phage-mediated horizontal gene transfer. However, little is known abo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01435-07
更新日期:2008-01-01 00:00:00
abstract::Various combinations of the genes cryIVA (cry4A), cryIVD (cry11A), and p20 from Bacillus thuringiensis subsp. israelensis were introduced into the nitrogen-fixing cyanobacterium Anabaena sp. strain PCC 7120 by means of Escherichia coli-Anabaena shuttle vector pRL488p and were expressed under control of two tandem stro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.12.4971-4974.1997
更新日期:1997-12-01 00:00:00
abstract::Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01962-10
更新日期:2011-03-01 00:00:00
abstract::An enzyme-linked immunosorbent assay was developed for the detection of whole cells of methanogens in samples from anaerobic continuously stirred tank digesters treating slurries of solid waste. The assay was found to allow for quantitative analysis of the most important groups of methanogens in samples from anaerobic...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.5.2001-2006.1997
更新日期:1997-05-01 00:00:00
abstract::Crude extracts from 14 species of sulfate-reducing bacteria comprising the genera Desulfovibrio, Desulfotomaculum, Desulfobulbus, and Desulfosarcina and from three species of sulfide-oxidizing bacteria were tested in an enzyme-linked immunosorbent assay with polyclonal antisera to adenosine 5'-phosphosulfate reductase...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.3.727-733.1991
更新日期:1991-03-01 00:00:00