Fluorescence in situ hybridization and spectral imaging of coral-associated bacterial communities.

abstract：:The accumulation and fate of model microbial "pathogens" within a drinking-water distribution system was investigated in naturally grown biofilms formed in a novel pilot-scale water distribution system provided with chlorinated and UV-treated water. Biofilms were exposed to 1-mum hydrophilic and hydrophobic microspher...

journal_title：Applied and environmental microbiology

authors： Långmark J,Storey MV,Ashbolt NJ,Stenström TA

更新日期：2005-02-01 00:00:00

abstract：:The purpose of this study was to examine host distribution patterns among fecal bacteria in the order Bacteroidales, with the goal of using endemic sequences as markers for fecal source identification in aquatic environments. We analyzed Bacteroidales 16S rRNA gene sequences from the feces of eight hosts: human, bovin...

journal_title：Applied and environmental microbiology

authors： Dick LK,Bernhard AE,Brodeur TJ,Santo Domingo JW,Simpson JM,Walters SP,Field KG

更新日期：2005-06-01 00:00:00

abstract：:We investigated the effects of copper on the structure and physiology of freshwater biofilm microbial communities. For this purpose, biofilms that were grown during 4 weeks in a shallow, slightly polluted ditch were exposed, in aquaria in our laboratory, to a range of copper concentrations (0, 1, 3, and 10 microM). De...

journal_title：Applied and environmental microbiology

authors： Massieux B,Boivin ME,Van Den Ende FP,Langenskiöld J,Marvan P,Barranguet C,Admiraal W,Laanbroek HJ,Zwart G

更新日期：2004-08-01 00:00:00

abstract：:Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

journal_title：Applied and environmental microbiology

authors： Nielsen KM,Bones AM,Van Elsas JD

更新日期：1997-10-01 00:00:00

abstract：:Inactivation of aflatoxin B1 was studied by using gamma radiation and hydrogen peroxide. A 100-krad dose of gamma radiation was sufficient to inactivate 50 micrograms of aflatoxin B1 in the presence of 5% hydrogen peroxide, and 400 krad was required for total degradation of 100 micrograms of aflatoxin in the same syst...

journal_title：Applied and environmental microbiology

authors： Patel UD,Govindarajan P,Dave PJ

更新日期：1989-02-01 00:00:00

abstract：:A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...

journal_title：Applied and environmental microbiology

authors： Kharazmi M,Sczesny S,Blaut M,Hammes WP,Hertel C

更新日期：2003-10-01 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:Lactobacillus plantarum is an attractive candidate for bioprocessing of lignocellulosic biomass due to its high metabolic variability, including its ability to ferment both pentoses and hexoses, as well as its high acid tolerance, a quality often utilized in industrial processes. This bacterium grows naturally on biom...

journal_title：Applied and environmental microbiology

authors： Moraïs S,Shterzer N,Grinberg IR,Mathiesen G,Eijsink VG,Axelsson L,Lamed R,Bayer EA,Mizrahi I

更新日期：2013-09-01 00:00:00

abstract：:Chitin is an abundant biopolymer whose degradation is mediated primarily by bacterial chitinases. We developed a degenerate PCR primer set to amplify a approximately 900-bp fragment of family 18, group I chitinase genes and used it to retrieve these gene fragments from environmental samples. Clone libraries of presump...

journal_title：Applied and environmental microbiology

authors： LeCleir GR,Buchan A,Hollibaugh JT

更新日期：2004-12-01 00:00:00

abstract：:The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...

journal_title：Applied and environmental microbiology

authors： Klein M,Brown L,Tucker RW,Ashbolt NJ,Stuetz RM,Roser DJ

更新日期：2010-10-01 00:00:00

abstract：:Streptococcus suis is an important pathogen of swine which occasionally infects humans as well. There are 35 serotypes known for this organism, and it would be desirable to develop rapid methods methods to identify and differentiate the strains of this species. To that effect, partial chaperonin 60 gene sequences were...

journal_title：Applied and environmental microbiology

authors： Brousseau R,Hill JE,Préfontaine G,Goh SH,Harel J,Hemmingsen SM

更新日期：2001-10-01 00:00:00

abstract：:A simplified X-ray film method for detecting bacterial production of Hgo by Escherichia coli was developed. There was excellent agreement of results between this X-ray film method and radioactive mercury volatilization assays. Although not quantitative, the X-ray film method has the advantages of being rapid, simple t...

journal_title：Applied and environmental microbiology

authors： Nakamura K,Nakahara H

更新日期：1988-11-01 00:00:00

abstract：:We describe a new enzymatic functionality for the surface layer (S-layer) of Lactobacillus acidophilus ATCC 4356, namely, an endopeptidase activity against the cell wall of Salmonella enterica serovar Newport, assayed via zymograms and identified by Western blotting. Based on amino acid sequence comparisons, the hydro...

journal_title：Applied and environmental microbiology

authors： Prado Acosta M,Mercedes Palomino M,Allievi MC,Sanchez Rivas C,Ruzal SM

更新日期：2008-12-01 00:00:00

abstract：:1,2-Dibromoethane (EDB), which is a groundwater contaminant in areas where it was once used as a soil fumigant, was shown to be degraded aerobically by microorganisms in two types of surface soils from an EDB-contaminated groundwater discharge area. At initial concentrations of 6 to 8 mug/liter, EDB was degraded in a ...

journal_title：Applied and environmental microbiology

authors： Pignatello JJ

更新日期：1986-03-01 00:00:00

abstract：:The organization and species composition of bacterial trophic groups associated with lactose biomethanation were investigated in a whey-processing chemostat by enumeration, isolation, and general characterization studies. The bacteria were spatially organized as free-living forms and as self-immobilized forms appearin...

journal_title：Applied and environmental microbiology

authors： Chartrain M,Zeikus JG

更新日期：1986-01-01 00:00:00

abstract：:The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

journal_title：Applied and environmental microbiology

authors： Jørgensen NO,Kroer N,Coffin RB

更新日期：1994-11-01 00:00:00

abstract：:Temperature and relative humidity are major factors determining virus inactivation in the environment. This article reviews inactivation data regarding coronaviruses on surfaces and in liquids from published studies and develops secondary models to predict coronaviruses inactivation as a function of temperature and re...

journal_title：Applied and environmental microbiology

authors： Guillier L,Martin-Latil S,Chaix E,Thébault A,Pavio N,Le Poder S,Batéjat C,Biot F,Koch L,Schaffner DW,Sanaa M,Covid-19 Emergency Collective Expert Appraisal Group.

更新日期：2020-09-01 00:00:00

abstract：:The transposon Tn916 and self-mobilizing plasmid pAM beta 1 were conjugated from Enterococcus faecalis to the ruminal bacterium Streptococcus bovis JB1. Transconjugants were identified by resistance to tetracycline (Tn916) or erythromycin (pAM beta 1) and by Southern hybridization analyses. Transfer frequencies were 7...

journal_title：Applied and environmental microbiology

authors： Hespell RB,Whitehead TR

更新日期：1991-09-01 00:00:00

abstract：:A fixed-bed loop, high-rate anaerobic bioreactor treating sulfite evaporator condensate was sampled when it reached steady state and afterwards following perturbations during a 14-month period. By using immunotechnology, it was observed that shifts in methanogenic subpopulations occurred in association with perturbati...

journal_title：Applied and environmental microbiology

authors： Macario AJ,Conway de Macario E,Ney U,Schoberth SM,Sahm H

更新日期：1989-08-01 00:00:00

abstract：:In many cities, the feral rock dove is an abundant bird species that can harbor Chlamydophila psittaci. We determined the prevalence and genotype of C. psittaci in fresh fecal samples from feral pigeons in Amsterdam, The Netherlands. The prevalence was 7.9% overall (26/331; 95% confidence interval, 5 to 11). Ten genot...

journal_title：Applied and environmental microbiology

authors： Heddema ER,Ter Sluis S,Buys JA,Vandenbroucke-Grauls CM,van Wijnen JH,Visser CE

更新日期：2006-06-01 00:00:00

abstract：:Gamma irradiation is a nonthermal processing technology that has been used for the preservation of a variety of food products. This technology has been shown to effectively inactivate bacterial pathogens. Currently, the FDA has approved doses of up to 4.0 kGy to control food-borne pathogens in fresh iceberg lettuce an...

journal_title：Applied and environmental microbiology

authors： Feng K,Divers E,Ma Y,Li J

更新日期：2011-05-01 00:00:00

abstract：:Exudates from Streptomyces griseoflavus Tü 2484 effectively mediated electron transfer between hydrogen sulfide and various nitrobenzenes. In general, pseudo-first-order kinetics were observed, except for the initial phase of the reaction at higher pH values. Under fixed pH and Dh conditions, linear free energy relati...

journal_title：Applied and environmental microbiology

authors： Glaus MA,Heijman CG,Schwarzenbach RP,Zeyer J

更新日期：1992-06-01 00:00:00

abstract：:Products observed during anaerobic cyanide transformation are consistent with a hydrolytic pathway (HCN + H2O <--> HCONH2 + H2O <--> HCOOH + NH3). Formate, the most frequently observed product, was generally converted to bicarbonate. Formamide was rapidly hydrolyzed to formate upon exposure to the anaerobic consortium...

journal_title：Applied and environmental microbiology

authors： Fallon RD

更新日期：1992-09-01 00:00:00

abstract：:Enterohemorrhagic Escherichia coli (EHEC) causes serious foodborne disease worldwide. It produces the very potent Shiga toxin 2 (Stx2). The Stx2-encoding genes are located on a prophage, and production of the toxin is linked to the synthesis of Stx phages. There is, currently, no good treatment for EHEC infections, as...

journal_title：Applied and environmental microbiology

authors： Kijewski A,Witsø IL,Iversen H,Rønning HT,L'Abée-Lund T,Wasteson Y,Lindbäck T,Aspholm M

更新日期：2020-11-24 00:00:00

abstract：:Resistance to bile is a prerequisite property of the gastrointestinal bacterial flora. Bile acids are powerful detergents, and resistance to sodium dodecyl sulfate (SDS) has therefore often been considered relevant to studies of bile resistance. We have studied the effects of bovine bile (BB) and SDS on Enterococcus f...

journal_title：Applied and environmental microbiology

authors： Solheim M,Aakra A,Vebø H,Snipen L,Nes IF

更新日期：2007-09-01 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00

abstract：:Nisin is a small gene-encoded antimicrobial protein produced by Lactococcus lactis that contains unusual dehydroalanine and dehydrobutyrine residues. The reactivity of these residues toward nucleophiles was explored by reacting nisin with a variety of mercaptans. The kinetics of reaction with 2-mercaptoethane-sulfonat...

journal_title：Applied and environmental microbiology

authors： Liu W,Hansen JN

更新日期：1990-08-01 00:00:00

abstract：:Streptococcus mutans is a key cariogenic bacterium responsible for the initiation of tooth decay. Biofilm formation is a crucial virulence property. We discovered a putative glycosyltransferase, SMU_833, in S. mutans capable of modulating dynamic interactions between two key biofilm matrix components, glucan and extra...

journal_title：Applied and environmental microbiology

authors： Rainey K,Michalek SM,Wen ZT,Wu H

更新日期：2019-02-20 00:00:00

abstract：:A dense exopolysaccharide (EPS) matrix is crucial for cyanobacterial survival in terrestrial xeric environments, in which cyanobacteria undergo frequent expansion and shrinkage processes during environmental desiccation-rehydration cycles. However, it is unclear how terrestrial cyanobacteria coordinate the structural ...

journal_title：Applied and environmental microbiology

authors： Liu W,Cui L,Xu H,Zhu Z,Gao X

更新日期：2017-10-31 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00