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Accumulation and fate of microorganisms and microspheres in biofilms formed in a pilot-scale water distribution system.

Abstract：

:The accumulation and fate of model microbial "pathogens" within a drinking-water distribution system was investigated in naturally grown biofilms formed in a novel pilot-scale water distribution system provided with chlorinated and UV-treated water. Biofilms were exposed to 1-mum hydrophilic and hydrophobic microspheres, Salmonella bacteriophages 28B, and Legionella pneumophila bacteria, and their fate was monitored over a 38-day period. The accumulation of model pathogens was generally independent of the biofilm cell density and was shown to be dependent on particle surface properties, where hydrophilic spheres accumulated to a larger extent than hydrophobic ones. A higher accumulation of culturable legionellae was measured in the chlorinated system compared to the UV-treated system with increasing residence time. The fate of spheres and fluorescence in situ hybridization-positive legionellae was similar and independent of the primary disinfectant applied and water residence time. The more rapid loss of culturable legionellae compared to the fluorescence in situ hybridization-positive legionellae was attributed to a loss in culturability rather than physical desorption. Loss of bacteriophage 28B plaque-forming ability together with erosion may have affected their fate within biofilms in the pilot-scale distribution system. The current study has demonstrated that desorption was one of the primary mechanisms affecting the loss of microspheres, legionellae, and bacteriophage from biofilms within a pilot-scale distribution system as well as disinfection and biological grazing. In general, two primary disinfection regimens (chlorination and UV treatment) were not shown to have a measurable impact on the accumulation and fate of model microbial pathogens within a water distribution system.

journal_name

Appl Environ Microbiol

journal_title

Applied and environmental microbiology

authors

Långmark J,Storey MV,Ashbolt NJ,Stenström TA

doi

10.1128/AEM.71.2.706-712.2005

keywords:

subject

Has Abstract

pub_date

2005-02-01 00:00:00

pages

706-12

issue

eissn

0099-2240

issn

1098-5336

pii

71/2/706

journal_volume

pub_type

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Accumulation and fate of microorganisms and microspheres in biofilms formed in a pilot-scale water distribution system.