Characteristics of environmental isolates of Legionella pneumophila.

abstract：:Filamentous fungi are widely used for the production of homologous and heterologous proteins. Recently, there has been increasing interest in Aspergillus oryzae because of its ability to produce heterologous proteins in solid-state culture. To provide an overview of protein secretion by A. oryzae in solid-state cultur...

journal_title：Applied and environmental microbiology

authors： Oda K,Kakizono D,Yamada O,Iefuji H,Akita O,Iwashita K

更新日期：2006-05-01 00:00:00

abstract：:Injury induced in Escherichia coli cells by chlorination was studied from a physiological standpoint. Predictable and reproducible injury was found to occur rapidly in 0.5 mg of chlorine per liter and was reversible under nonselective conditions. There was an extended lag period in the growth of chlorinated cells not ...

journal_title：Applied and environmental microbiology

authors： Camper AK,McFeters GA

更新日期：1979-03-01 00:00:00

abstract：:It has been suggested (L. H. Harris, R. P. Desai, N. E. Welker, and E. T. Papoutsakis, Biotechnol. Bioeng. 67:1-11, 2000) that butyryl phosphate (BuP) is a regulator of solventogenesis in Clostridium acetobutylicum. Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild ty...

journal_title：Applied and environmental microbiology

authors： Zhao Y,Tomas CA,Rudolph FB,Papoutsakis ET,Bennett GN

更新日期：2005-01-01 00:00:00

abstract：:The ability of bifidobacteria to adhere to the intestine of the human host is considered to be important for efficient colonization and achieving probiotic effects. Bifidobacterium bifidum strains DSM20456 and MIMBb75 adhere well to the human intestinal cell lines Caco-2 and HT-29. The surface lipoprotein BopA was pre...

journal_title：Applied and environmental microbiology

authors： Kainulainen V,Reunanen J,Hiippala K,Guglielmetti S,Vesterlund S,Palva A,Satokari R

更新日期：2013-11-01 00:00:00

abstract：:Saccharomyces pastorianus strains are hybrids of Saccharomyces cerevisiae and Saccharomyces eubayanus that have been domesticated for centuries in lager beer brewing environments. As sequences and structures of S. pastorianus genomes are being resolved, molecular mechanisms and evolutionary origins of several industri...

journal_title：Applied and environmental microbiology

authors： Brouwers N,Brickwedde A,Gorter de Vries AR,van den Broek M,Weening SM,van den Eijnden L,Diderich JA,Bai FY,Pronk JT,Daran JG

更新日期：2019-10-30 00:00:00

abstract：:A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleoti...

journal_title：Applied and environmental microbiology

authors： Andrew M,Kohn LM

更新日期：2009-09-01 00:00:00

abstract：:Eight concentration and purification methods were evaluated to determine percentages of recovery of Cryptosporidium parvum oocysts from calf feces. The NaCl flotation method generally resulted in the highest percentages of recovery. Based on the percentages of recovery, the amounts of fecal debris in the final oocyst ...

journal_title：Applied and environmental microbiology

authors： Kuczynska E,Shelton DR

更新日期：1999-07-01 00:00:00

abstract：:DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific prob...

journal_title：Applied and environmental microbiology

authors： Shin BS,Park SH,Choi SK,Koo BT,Lee ST,Kim JI

更新日期：1995-06-01 00:00:00

abstract：:The transcript abundances of nitrate transporter genes (Nrt2) were proposed as potential markers for nitrogen deficiency in marine diatoms. To correctly quantify diatom Nrt2 mRNA in the East China Sea (ECS), we utilized both mixed-species sequencing and single-cell PCR to expand the sequence database for this region. ...

journal_title：Applied and environmental microbiology

authors： Kang LK,Wang HF,Chang J

更新日期：2011-01-01 00:00:00

abstract：:Anaerobic degradation of 2-methylnaphthalene was investigated with a sulfate-reducing enrichment culture. Metabolite analyses revealed two groups of degradation products. The first group comprised two succinic acid adducts which were identified as naphthyl-2-methyl-succinic acid and naphthyl-2-methylene-succinic acid ...

journal_title：Applied and environmental microbiology

authors： Annweiler E,Materna A,Safinowski M,Kappler A,Richnow HH,Michaelis W,Meckenstock RU

更新日期：2000-12-01 00:00:00

abstract：:The diversity of sulfate-reducing bacteria (SRB) in brackish sediment was investigated using small-subunit rRNA and dissimilatory sulfite reductase (DSR) gene clone libraries and cultivation. The phylogenetic affiliation of the most commonly retrieved clones for both genes was strikingly similar and produced Desulfosa...

journal_title：Applied and environmental microbiology

authors： Joulian C,Ramsing NB,Ingvorsen K

更新日期：2001-07-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:Simkania negevensis is a recently discovered chlamydia-like intracellular microorganism which has been associated with bronchiolitis in infants and with community-acquired pneumonia in adults; a high seroprevalence of antibodies to the microorganism has been found in various population groups. S. negevensis can be gro...

journal_title：Applied and environmental microbiology

authors： Kahane S,Platzner N,Dvoskin B,Itzhaki A,Friedman MG

更新日期：2004-06-01 00:00:00

abstract：:The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2001-03-01 00:00:00

abstract：:Algal-bacterial mats which grow in the effluent channels of alkaline hot springs provided an environment suitable for studying natural thermophilic methane producing bacteria. Methane was rapidly produced in cores taken from the meat and appeared to be an end product of decomposition of the algal-bacterial organic mat...

journal_title：Applied and environmental microbiology

authors： Ward DM

更新日期：1978-06-01 00:00:00

abstract：:Mycobiota growing on food is often beneficial for the ripening and development of the specific flavor characteristics of the product, but it can also be harmful due to the production of undesirable compounds such as mycotoxins or antibiotics. Some of the fungi most frequently isolated from fermented and cured meat pro...

journal_title：Applied and environmental microbiology

authors： Laich F,Fierro F,Martín JF

更新日期：2002-03-01 00:00:00

abstract：:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated ...

journal_title：Applied and environmental microbiology

authors： Tannock GW

更新日期：1989-02-01 00:00:00

abstract：:The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

journal_title：Applied and environmental microbiology

authors： Jørgensen NO,Kroer N,Coffin RB

更新日期：1994-11-01 00:00:00

abstract：:Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

journal_title：Applied and environmental microbiology

authors： Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-09-17 00:00:00

abstract：:Soybean trypsin inhibitors are thought to ward off pathogens. Studies with aflatoxigenic strains of Aspergillus flavus and A. parasiticus, frequent soybean contaminants, revealed that trypsin inhibitors do not affect the growth of these fungi and aflatoxin production. Further, the availability of zinc, an essential mi...

journal_title：Applied and environmental microbiology

authors： Stössel P

更新日期：1986-07-01 00:00:00

abstract：:High-cell-density fermentation for industrial production of chemicals can impose numerous stresses on cells due to high substrate, product, and by-product concentrations; high osmolarity; reactive oxygen species; and elevated temperatures. There is a need to develop platform strains of industrial microorganisms that a...

journal_title：Applied and environmental microbiology

authors： Lennen RM,Herrgård MJ

更新日期：2014-10-01 00:00:00

abstract：:Some Shiga toxin-producing Escherichia coli (STEC) strains produce extracellular cellulose, a long polymer of glucose with β-1-4 glycosidic bonds. This study evaluated the efficacies of selected enzymatic and chemical treatments in inactivating STEC and degrading/removing the cellulose on STEC surfaces. Six cellulose-...

journal_title：Applied and environmental microbiology

authors： Park YJ,Chen J

更新日期：2011-12-01 00:00:00

abstract：:Three cases of Mycobacterium avium complex-related lung disorders were associated with two poorly maintained spa pools by genotypic investigations. Inadequate disinfection of the two spas had reduced the load of environmental bacteria to less than 1 CFU/ml but allowed levels of M. avium complex of 4.3 x 10(4) and 4.5 ...

journal_title：Applied and environmental microbiology

authors： Lumb R,Stapledon R,Scroop A,Bond P,Cunliffe D,Goodwin A,Doyle R,Bastian I

更新日期：2004-08-01 00:00:00

abstract：:A mechanistic understanding of how new phenotypes develop to overcome the loss of a gene product provides valuable insight on both the metabolic and regulatory functions of the lost gene. The pgi gene, whose product catalyzes the second step in glycolysis, was deleted in a growth-optimized Escherichia coli K-12 MG1655...

journal_title：Applied and environmental microbiology

authors： McCloskey D,Xu S,Sandberg TE,Brunk E,Hefner Y,Szubin R,Feist AM,Palsson BO

更新日期：2018-09-17 00:00:00

abstract：:Growing bacteria have a high concentration of ribosomes to ensure sufficient protein synthesis, which is necessary for genome replication and cellular division. To elucidate whether metabolic activity of soil microorganisms is coupled with growth, we investigated the relationship between rRNA and DNA synthesis in a so...

journal_title：Applied and environmental microbiology

authors： Papp K,Hungate BA,Schwartz E

更新日期：2018-04-02 00:00:00

abstract：:The enzyme levanase encoded by the sacC gene from Bacillus subtilis was overexpressed in Escherichia coli with the strong, inducible tac promoter. The enzyme was purified from crude E. coli cell lysates by salting out with ammonium sulfate and chromatography on DEAE-Sepharose CL-6B, S-Sepharose, and MonoQ-Sepharose. T...

journal_title：Applied and environmental microbiology

authors： Wanker E,Huber A,Schwab H

更新日期：1995-05-01 00:00:00

abstract：:Although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. Progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. ...

journal_title：Applied and environmental microbiology

authors： Heller D,Helmerhorst EJ,Gower AC,Siqueira WL,Paster BJ,Oppenheim FG

更新日期：2016-01-08 00:00:00

abstract：:Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

journal_title：Applied and environmental microbiology

authors： Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

更新日期：1980-12-01 00:00:00

abstract：:The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat. ...

journal_title：Applied and environmental microbiology

authors： Makino S,Okada Y,Maruyama T

更新日期：1995-10-01 00:00:00

abstract：:Virulence gene profiles of atypical enteropathogenic Escherichia coli (aEPEC) and Shiga toxin-producing E. coli (STEC) from cattle, sheep, and humans were examined to determine the relationship between pathotypes. Shared virulence factors (intimin, EHEC hemolysin, serine protease, and a type II secretion system) were ...

journal_title：Applied and environmental microbiology

authors： Cookson AL,Cao M,Bennett J,Nicol C,Thomson-Carter F,Attwood GT

更新日期：2010-06-01 00:00:00