Diversity of phytoplankton nitrate transporter sequences from isolated single cells and mixed samples from the East China Sea and mRNA quantification.

abstract：:Biogeochemical transformations occurring in the anoxic zones of stratified sedimentary microbial communities can profoundly influence the isotopic and organic signatures preserved in the fossil record. Accordingly, we have determined carbon isotope discrimination that is associated with both heterotrophic and lithotro...

journal_title：Applied and environmental microbiology

authors： Londry KL,Des Marais DJ

更新日期：2003-05-01 00:00:00

abstract：:The ability of fractions of dissolved organic carbon (DOC) of different molecular weights (MW) to support bacterial growth was studied in batch culture experiments. Natural pelagic bacteria were inoculated into particle-free (0.2-mum filtered) water, taken from 10 oligotrophic lakes of differing humic content, and eit...

journal_title：Applied and environmental microbiology

authors： Tranvik LJ

更新日期：1990-06-01 00:00:00

abstract：:The pathways responsible for cell wall polysaccharide biosynthesis are vital in eukaryotic microorganisms. The corresponding synthases are potential targets of inhibitors such as fungicides. Despite their fundamental and economical importance, most polysaccharide synthases are not well characterized, and their molecul...

journal_title：Applied and environmental microbiology

authors： Briolay A,Bouzenzana J,Guichardant M,Deshayes C,Sindt N,Bessueille L,Bulone V

更新日期：2009-04-01 00:00:00

abstract：:The kinetics of the enzymatic formation of 7,8-epoxy-1-octene, 1,2-7,8-diepoxyoctane, and 1,2-epoxyoctane by growing and resting cell suspensions of Pseudomonas oleovorans are described. Formation of 1,2-epoxyoctane occurs concurrently with exponential growth on 1-octene, providing that 1-octene is in excess. Conversi...

journal_title：Applied and environmental microbiology

authors： Schwartz RD,McCoy CJ

更新日期：1976-01-01 00:00:00

abstract：:Cotton flowers in replicate plots in two fields near Phoenix, Ariz., were tagged in June at the beginning of the flowering period. Flowers or developing bolls from these tagged flowers were inoculated on the involucral (bracteal) nectaries with dry spores of Aspergillus flavus. The bolls were harvested as they matured...

journal_title：Applied and environmental microbiology

authors： Klich MA,Chmielewski MA

更新日期：1985-09-01 00:00:00

abstract：:Upstream of the Streptomyces coelicolor A3(2) chitinase G gene, a small gene (named chb3) is located whose deduced product shares 37% identical amino acids with the previously described CHB1 protein from Streptomyces olivaceoviridis. The chb3 gene and its upstream region were cloned in a multicopy vector and transform...

journal_title：Applied and environmental microbiology

authors： Saito A,Miyashita K,Biukovic G,Schrempf H

更新日期：2001-03-01 00:00:00

abstract：:Listeria monocytogenes does not produce siderophores for iron acquisition. We demonstrate that a number of microbial siderophores and natural iron-binding compounds are able to promote the growth of iron-starved L. monocytogenes. We suggest that the ability of L. monocytogenes to use a variety of exogenous siderophore...

journal_title：Applied and environmental microbiology

authors： Simon N,Coulanges V,Andre P,Vidon DJ

更新日期：1995-04-01 00:00:00

abstract：:Severe intoxications caused by the Bacillus cereus emetic toxin cereulide can hardly be prevented due to the ubiquitous distribution and heat resistance of spores and the extreme thermal and chemical stability of cereulide. It would therefore be desirable to inhibit cereulide synthesis during food manufacturing proces...

journal_title：Applied and environmental microbiology

authors： Frenzel E,Letzel T,Scherer S,Ehling-Schulz M

更新日期：2011-02-01 00:00:00

abstract：:The bacterial content of specially treated cottons used by other investigators to test human pulmonary responses to cotton dust was examined. Cotton from Lubbock, Tex. and Stoneville, Miss. were either (i) harvested by machine and handled as commercial bale cotton, (ii) harvested as closed bolls with bracts intact and...

journal_title：Applied and environmental microbiology

authors： Millner PD,Ericson KE,Marsh PB

更新日期：1982-08-01 00:00:00

abstract：:A PCR-based method and a reverse transcriptase PCR (RT-PCR)-based method were developed for the detection of pathogenic bacteria in organic waste, using Salmonella spp., Listeria monocytogenes, Yersinia enterocolitica, and Staphylococcus aureus as model organisms. In seeded organic waste samples, detection limits of l...

journal_title：Applied and environmental microbiology

authors： Burtscher C,Wuertz S

更新日期：2003-08-01 00:00:00

abstract：:We have previously reported that an acid tolerance response (ATR) can be induced in Streptococcus macedonicus cells at mid-log phase after autoacidification, transient exposure to acidic pH, or acid habituation, as well as at stationary phase. Here, we compared the transcriptional profiles of these epigenetic phenotyp...

journal_title：Applied and environmental microbiology

authors： Papadimitriou K,Boutou E,Zoumpopoulou G,Tarantilis PA,Polissiou M,Vorgias CE,Tsakalidou E

更新日期：2008-10-01 00:00:00

abstract：:Malonyl coenzyme A (malonyl-CoA) and methylmalonyl-CoA are the most common extender units for the biosynthesis of fatty acids and polyketides in Streptomyces, an industrially important producer of polyketides. Carboxylation of acetyl- and propionyl-CoAs is an essential source of malonyl- and methylmalonyl-CoAs; theref...

journal_title：Applied and environmental microbiology

authors： Lyu M,Cheng Y,Han X,Wen Y,Song Y,Li J,Chen Z

更新日期：2020-06-02 00:00:00

abstract：:An improved method of dot-blot hybridization to detect hepatitis A virus (HAV) was developed with single-stranded RNA (ssRNA) probes. Radioactive and nonradioactive ssRNA probes were generated by in vitro transcription of HAV templates inserted into the plasmid pGEM-1. 32P-labeled ssRNA probes were at least eightfold ...

journal_title：Applied and environmental microbiology

authors： Jiang X,Estes MK,Metcalf TG

更新日期：1987-10-01 00:00:00

abstract：:The introduction of "solids" (i.e., complementary foods) to the milk-only diet in early infancy affects the development of the gut microbiota. The aim of this study was to determine whether a "baby-led" approach to complementary feeding that encourages the early introduction of an adult-type diet results in alteration...

journal_title：Applied and environmental microbiology

authors： Leong C,Haszard JJ,Lawley B,Otal A,Taylor RW,Szymlek-Gay EA,Fleming EA,Daniels L,Fangupo LJ,Tannock GW,Heath AM

更新日期：2018-08-31 00:00:00

abstract：:In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

更新日期：2015-10-01 00:00:00

abstract：:Penicillium expansum isolated from meat and apples produced both patulin and citrinin. Toxin identity was confirmed by spectroscopic and physical methods. The mean lethal dose in chicken embryos was determined for toxins administered both singly and in various ratios. Data from simultaneous administration of mycotoxin...

journal_title：Applied and environmental microbiology

authors： Ciegler A,Vesonder RF,Jackson LK

更新日期：1977-04-01 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:We present a phylogenetic analysis of nine strains of symbiotic nitrogen-fixing bacteria isolated from nodules of tagasaste (Chamaecytisus proliferus) and other endemic woody legumes of the Canary Islands, Spain. These and several reference strains were characterized genotypically at different levels of taxonomic reso...

journal_title：Applied and environmental microbiology

authors： Vinuesa P,Rademaker JL,de Bruijn FJ,Werner D

更新日期：1998-06-01 00:00:00

abstract：:Although the biological conversion of nitrite to nitrate is a well-known process, studies of Nitrobacter populations are hindered by their physiological characteristics. This report describes a new method for detecting and counting Nitrobacter populations in situ with the PCR. Two primers from the 16S rRNA gene were u...

journal_title：Applied and environmental microbiology

authors： Degrange V,Bardin R

更新日期：1995-06-01 00:00:00

abstract：:Melanin is a pigment produced by organisms throughout all domains of life. Due to its unique physicochemical properties, biocompatibility, and biostability, there has been an increasing interest in the use of melanin for broad applications. In the vast majority of studies, melanin has been either chemically synthesize...

journal_title：Applied and environmental microbiology

authors： Wang Z,Tschirhart T,Schultzhaus Z,Kelly EE,Chen A,Oh E,Nag O,Glaser ER,Kim E,Lloyd PF,Charles PT,Li W,Leary D,Compton J,Phillips DA,Dhinojwala A,Payne GF,Vora GJ

更新日期：2020-02-18 00:00:00

abstract：:The ever-increasing industrial demand for biocatalysis necessitates innovations in the preparation and stabilization of biocatalysts. In this study, we demonstrated that beta-galactosidase (beta-Gal) displayed on Bacillus spores by fusion to the spore coat proteins (CotG) may be used as a whole-cell immobilized biocat...

journal_title：Applied and environmental microbiology

authors： Kwon SJ,Jung HC,Pan JG

更新日期：2007-04-01 00:00:00

abstract：:Biolog's identification system was used to identify 39 American Type Culture Collection reference taxa and 45 gram-negative isolates from water samples. Of the reference strains, 98% were identified to genus level and 76% to species level within 4 to 24 h. Identification of some authentic strains of Enterobacter, Kl...

journal_title：Applied and environmental microbiology

authors： Klingler JM,Stowe RP,Obenhuber DC,Groves TO,Mishra SK,Pierson DL

更新日期：1992-06-01 00:00:00

abstract：:This study investigated the efficiency of methane and ammonium for stimulating trichloroethylene (TCE) biodegradation in groundwater microcosms (flasks and batch exchange columns) at a psychrophilic temperature (12 degrees C) typical of shallow aquifers in the northern United States or a mesophilic temperature (24 deg...

journal_title：Applied and environmental microbiology

authors： Moran BN,Hickey WJ

更新日期：1997-10-01 00:00:00

abstract：:By taking advantage of MazF, an ACA codon-specific mRNA interferase, Escherichia coli cells can be converted into a bioreactor producing only a single protein of interest by using an ACA-less mRNA for the protein. In this single-protein production (SPP) system, we engineered MazF by replacing two tryptophan residues i...

journal_title：Applied and environmental microbiology

authors： Vaiphei ST,Mao L,Shimazu T,Park JH,Inouye M

更新日期：2010-09-01 00:00:00

abstract：:The extremely thermoacidophilic archaeon Metallosphaera sedula (optimum growth temperature, 73°C, pH 2.0) grows chemolithoautotrophically on metal sulfides or molecular hydrogen by employing the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) carbon fixation cycle. This cycle adds two CO2 molecules to acetyl coenzyme ...

journal_title：Applied and environmental microbiology

authors： Hawkins AB,Adams MW,Kelly RM

更新日期：2014-04-01 00:00:00

abstract：:Haa1 is a transcription factor that adapts Saccharomyces cerevisiae cells to weak organic acid stresses by activating the expression of various genes. Many of these genes encode membrane proteins, such as TPO2 and YRO2 How Haa1 is activated by weak acids is not clear. Here, we show that casein kinase I isoform Hrr25 i...

journal_title：Applied and environmental microbiology

authors： Collins ME,Black JJ,Liu Z

更新日期：2017-06-16 00:00:00

abstract：:A novel enterotoxin gene was cloned from Bacillus cereus FM1, and its nucleotide sequence was determined. Previously, a 45-kDa protein causing characteristic enterotoxin symptoms in higher animals had been isolated (K. Shinagawa, p. 181-193, in A. E. Pohland et al., ed., Microbial Toxins in Foods and Feeds, 1990) from...

journal_title：Applied and environmental microbiology

authors： Asano SI,Nukumizu Y,Bando H,Iizuka T,Yamamoto T

更新日期：1997-03-01 00:00:00

abstract：:The bphC and bphD genes of Pseudomonas putida involved in the catabolism of polychlorinated biphenyls or biphenyl were identified, localized, and studied for expression in Escherichia coli. This was achieved by cloning a 2.4-kilobase (kb) DNA fragment of recombinant cosmid pOH101 into HindIII site of pUC plasmids down...

journal_title：Applied and environmental microbiology

authors： Khan AA,Walia SK

更新日期：1990-04-01 00:00:00

abstract：:The ability of the facultative photoheterotroph Rhodobacter sphaeroides to tolerate and reduce high levels of tellurite in addition to at least 10 other rare earth metal oxides and oxyanions has considerable potential for detoxification and bioremediation of contaminated environments. We report the identification and ...

journal_title：Applied and environmental microbiology

authors： O'Gara JP,Gomelsky M,Kaplan S

更新日期：1997-12-01 00:00:00

abstract：:Carbon-starved cultures of strain Ant-300, a psychrophilic marine vibrio isolated from the Antarctic Convergence, were compared with their nonstarved counterparts for resistance to heat. Specifically, starved and unstarved cells were exposed to 17 degrees C, which is 4 degrees C above the maximum growth temperature, a...

journal_title：Applied and environmental microbiology

authors： Preyer JM,Oliver JD

更新日期：1993-08-01 00:00:00