Abstract:
:Carbon-starved cultures of strain Ant-300, a psychrophilic marine vibrio isolated from the Antarctic Convergence, were compared with their nonstarved counterparts for resistance to heat. Specifically, starved and unstarved cells were exposed to 17 degrees C, which is 4 degrees C above the maximum growth temperature, and compared with cells maintained at the optimum temperature (5 to 7 degrees C). Total cell counts, direct viable-cell counts, and plate counts were monitored. At a temperature of 17 degrees C, viability (as indicated by plate counts) was lost within 40 h, with direct viable-cell counts indicating less than 5% viability at this time. However, when cells were carbon starved for 1 week prior to heat challenge, significant plateability was maintained for more than 6 days; direct viable-cell counts of starved cells maintained at 17 degrees C indicated the presence of viable cells for at least 12 days. Because starvation is the normal physiological state of copiotrophic, heterotrophic bacteria in oligotrophic marine waters, these data suggest that starvation conditions may be a significant factor in providing heat tolerance to psychrophiles.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Preyer JM,Oliver JDdoi
10.1128/AEM.59.8.2653-2656.1993keywords:
subject
Has Abstractpub_date
1993-08-01 00:00:00pages
2653-6issue
8eissn
0099-2240issn
1098-5336journal_volume
59pub_type
杂志文章abstract::In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...
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更新日期:1990-01-01 00:00:00
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