Ecological behavior of Lactobacillus reuteri 100-23 is affected by mutation of the luxS gene.

Abstract:

:The luxS gene of Lactobacillus reuteri 100-23C was amplified by PCR, cloned, and then sequenced. To define a physiological and ecological role for the luxS gene in L. reuteri 100-23C, a luxS mutant was constructed by insertional mutagenesis. The luxS mutant did not produce autoinducers AI-2 or AI-3. Complementation of the luxS mutation by a plasmid construct containing luxS restored AI-2 and AI-3 synthesis. In vitro experiments revealed that neither the growth rate, nor the cell yield, nor cell survival in the stationary phase were compromised in the luxS mutant relative to the wild type and complemented mutant. The ATP content of exponentially growing cells of the luxS mutant was, however, 65% of that of wild-type cells. Biofilms formed by the luxS mutant on plastic surfaces in a bioreactor were thicker than those formed by the wild type. Biofilm thickness was not restored to wild-type values by the addition of purified AI-2 to the culture medium. In vivo experiments, conducted with ex-Lactobacillus-free mice, showed that biofilms formed by the mutant strain on the epithelial surface of the forestomach were approximately twice as thick as those formed by the wild type. The ecological performance of the luxS mutant, when in competition with L. reuteri strain 100-93 in the mouse cecum, was reduced compared to that of a xylA mutant of 100-23C. These results demonstrate that LuxS influences important ecological attributes of L. reuteri 100-23C, the consequences of which are niche specific.

journal_name

Appl Environ Microbiol

authors

Tannock GW,Ghazally S,Walter J,Loach D,Brooks H,Cook G,Surette M,Simmers C,Bremer P,Dal Bello F,Hertel C

doi

10.1128/AEM.71.12.8419-8425.2005

keywords:

subject

Has Abstract

pub_date

2005-12-01 00:00:00

pages

8419-25

issue

12

eissn

0099-2240

issn

1098-5336

pii

71/12/8419

journal_volume

71

pub_type

杂志文章
  • Recombineering using RecTE from Pseudomonas syringae.

    abstract::In this report, we describe the identification of functions that promote genomic recombination of linear DNA introduced into Pseudomonas cells by electroporation. The genes encoding these functions were identified in Pseudomonas syringae pv. syringae B728a based on similarity to the lambda Red Exo/Beta and RecET prote...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00911-10

    authors: Swingle B,Bao Z,Markel E,Chambers A,Cartinhour S

    更新日期:2010-08-01 00:00:00

  • Engineering Escherichia coli Nicotinic Acid Mononucleotide Adenylyltransferase for Fully Active Amidated NAD Biosynthesis.

    abstract::NAD and its reduced form NADH function as essential redox cofactors and have major roles in determining cellular metabolic features. NAD can be synthesized through the deamidated and amidated pathways, for which the key reaction involves adenylylation of nicotinic acid mononucleotide (NaMN) and nicotinamide mononucleo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00692-17

    authors: Wang X,Zhou YJ,Wang L,Liu W,Liu Y,Peng C,Zhao ZK

    更新日期:2017-06-16 00:00:00

  • Wolbachia strain wAlbB enhances infection by the rodent malaria parasite Plasmodium berghei in Anopheles gambiae mosquitoes.

    abstract::Wolbachia, a common bacterial endosymbiont of insects, has been shown to protect its hosts against a wide range of pathogens. However, not all strains exert a protective effect on their host. Here we assess the effects of two divergent Wolbachia strains, wAlbB from Aedes albopictus and wMelPop from Drosophila melanoga...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06751-11

    authors: Hughes GL,Vega-Rodriguez J,Xue P,Rasgon JL

    更新日期:2012-03-01 00:00:00

  • D-values of Bacillus pumilus spores on irradiated devices (inoculated product).

    abstract::The D-values of Bacillus pumilus spores on various devices ranged from 0.14 to 0.23 Mrads. The majority of devices displayed D-values equal to or less than the value obtained on filter paper. Increased resistivity was also encountered. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.36.2.392-393.1978

    authors: Prince HN

    更新日期:1978-08-01 00:00:00

  • Exposure of Saccharomyces cerevisiae to acetaldehyde induces sulfur amino acid metabolism and polyamine transporter genes, which depend on Met4p and Haa1p transcription factors, respectively.

    abstract::Acetaldehyde is a toxic compound produced by Saccharomyces cerevisiae cells under several growth conditions. The adverse effects of this molecule are important, as significant amounts accumulate inside the cells. By means of global gene expression analyses, we have detected the effects of acetaldehyde addition in the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.4.1913-1922.2004

    authors: Aranda A,del Olmo ML

    更新日期:2004-04-01 00:00:00

  • Inhibitor Evaluation with Immobilized Nitrobacter agilis Cells.

    abstract::Nitrobacter agilis was entrapped in calcium alginate beads and used as a floating bed supplied with a continuous flow of nitrite medium. Complete nitrite oxidation was achieved within 30 h, and the system could be maintained for at least 210 h. The immobilized Nitrobacter system was subjected to sulfur oxyanions, acid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.6.1231-1235.1986

    authors: Tsai YL,Schlasner SM,Tuovinen OH

    更新日期:1986-12-01 00:00:00

  • Purification, characterization, and mode of action of endoxylanases 1 and 2 from Fibrobacter succinogenes S85.

    abstract::Two different endoxylanases (1,4-beta-D-xylan xylanohydrolases, EC 3.2.1.8), designated 1 and 2, have been purified by column chromatography to apparent homogeneity from the nonsedimentable extracellular culture fluid of the strictly anaerobic, ruminal bacterium Fibrobacter succinogenes S85 grown on crystalline cellul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.1.157-168.1992

    authors: Matte A,Forsberg CW

    更新日期:1992-01-01 00:00:00

  • In situ analysis of denitrifying toluene- and m-xylene-degrading bacteria in a diesel fuel-contaminated laboratory aquifer column.

    abstract::A diesel fuel-contaminated aquifer was bioremediated in situ by the injection of oxidants (O2 and NO3-) and nutrients in order to stimulate microbial activity. After 3.5 years of remediation, an aquifer sample was excavated and the material was used (i) to isolate bacterial strains able to grow on selected hydrocarbon...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.6.2136-2141.1997

    authors: Hess A,Zarda B,Hahn D,Häner A,Stax D,Höhener P,Zeyer J

    更新日期:1997-06-01 00:00:00

  • Effects of bacterial antibiotic production on rhizosphere microbial communities from a culture-independent perspective

    abstract::The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.5020-5022.1998

    authors: Robleto EA,Borneman J,Triplett EW

    更新日期:1998-12-01 00:00:00

  • Production and characterization of antibodies against nivalenol tetraacetate.

    abstract::Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.4.1026-1030.1991

    authors: Wang CR,Chu FS

    更新日期:1991-04-01 00:00:00

  • Heterologous Production of Cyanobacterial Mycosporine-Like Amino Acids Mycosporine-Ornithine and Mycosporine-Lysine in Escherichia coli.

    abstract::Mycosporine-like amino acids (MAAs) are an important class of secondary metabolites known for their protection against UV radiation and other stress factors. Cyanobacteria produce a variety of MAAs, including shinorine, the active ingredient in many sunscreen creams. Bioinformatic analysis of the genome of the soil-dw...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01632-16

    authors: Katoch M,Mazmouz R,Chau R,Pearson LA,Pickford R,Neilan BA

    更新日期:2016-09-30 00:00:00

  • Isolation and characterization of a novel antialgal allelochemical from Phragmites communis.

    abstract::Antialgal allelochemicals were isolated from Phragmites communis Tris. The isolated allelopathic fraction showed strong inhibition activity on the growth of Chlorella pyrenoidosa and Microcystis aeruginosa but had no inhibition on Chlorella vulgaris. The 50% effective concentrations (EC50) of the allelopathic fraction...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.6545-6553.2005

    authors: Li FM,Hu HY

    更新日期:2005-11-01 00:00:00

  • Respiration of 13C-labeled substrates added to soil in the field and subsequent 16S rRNA gene analysis of 13C-labeled soil DNA.

    abstract::Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1614-1622.2003

    authors: Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

    更新日期:2003-03-01 00:00:00

  • Genetic (In)stability of 2,6-Dichlorobenzamide Catabolism in Aminobacter sp. Strain MSH1 Biofilms under Carbon Starvation Conditions.

    abstract::Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00137-17

    authors: Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

    更新日期:2017-05-17 00:00:00

  • Chloroflexi CL500-11 Populations That Predominate Deep-Lake Hypolimnion Bacterioplankton Rely on Nitrogen-Rich Dissolved Organic Matter Metabolism and C1 Compound Oxidation.

    abstract::The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03014-15

    authors: Denef VJ,Mueller RS,Chiang E,Liebig JR,Vanderploeg HA

    更新日期:2015-12-18 00:00:00

  • Removal of endotoxin from water by microfiltration through a microporous polyethylene hollow-fiber membrane.

    abstract::The microporous polyethylene hollow-fiber membrane has a unique microfibrile structure throughout its depth and has been found to possess the functions of filtration and adsorption of endotoxin in water. The membrane has a maximum pore diameter of approximately 0.04 micron, a diameter which is within the range of micr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.4.813-820.1986

    authors: Sawada Y,Fujii R,Igami I,Kawai A,Kamiki T,Niwa M

    更新日期:1986-04-01 00:00:00

  • Curvularia lunata, a New Source of Cytochalasin B.

    abstract::A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.4.967-971.1981

    authors: Wells JM,Cole RJ,Cutler HC,Spalding DH

    更新日期:1981-04-01 00:00:00

  • Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization.

    abstract::The objective of this study was to explore the long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil. Soil from the following six treatments was analyzed in an experimental field site established in 1956: cattle m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8335-8343.2005

    authors: Enwall K,Philippot L,Hallin S

    更新日期:2005-12-01 00:00:00

  • Nitrogen-fixing nodules with Ensifer adhaerens harboring Rhizobium tropici symbiotic plasmids.

    abstract::Ensifer adhaerens is a soil bacterium that attaches to other bacteria and may cause lysis of these other bacteria. Based on the sequence of its small-subunit rRNA gene, E. adhaerens is related to Sinorhizobium spp. E. adhaerens ATCC 33499 did not nodulate Phaseolus vulgaris (bean) or Leucaena leucocephala, but with sy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.3264-3268.2001

    authors: Rogel MA,Hernández-Lucas I,Kuykendall LD,Balkwill DL,Martinez-Romero E

    更新日期:2001-07-01 00:00:00

  • Disparate independent genetic events disrupt the secondary metabolism gene perA in certain symbiotic Epichloë species.

    abstract::Peramine is an insect-feeding deterrent produced by Epichloë species in symbiotic association with C3 grasses. The perA gene responsible for peramine synthesis encodes a two-module nonribosomal peptide synthetase. Alleles of perA are found in most Epichloë species; however, peramine is not produced by many perA-contai...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03721-14

    authors: Berry D,Takach JE,Schardl CL,Charlton ND,Scott B,Young CA

    更新日期:2015-04-01 00:00:00

  • Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.

    abstract::Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.901-908.2003

    authors: Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

    更新日期:2003-02-01 00:00:00

  • Listeria monocytogenes EGD-e biofilms: no mushrooms but a network of knitted chains.

    abstract::Listeria monocytogenes is a food pathogen that can attach on most of the surfaces encountered in the food industry. Biofilms are three-dimensional microbial structures that facilitate the persistence of pathogens on surfaces, their resistance toward antimicrobials, and the final contamination of processed goods. So fa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00255-08

    authors: Rieu A,Briandet R,Habimana O,Garmyn D,Guzzo J,Piveteau P

    更新日期:2008-07-01 00:00:00

  • Serotype Diversity and Antimicrobial Resistance among Salmonella enterica Isolates from Patients at an Equine Referral Hospital.

    abstract::Although Salmonella enterica can produce life-threatening colitis in horses, certain serotypes are more commonly associated with clinical disease. Our aim was to evaluate the proportional morbidity attributed to different serotypes, as well as the phenotypic and genotypic antimicrobial resistance (AMR) of Salmonella i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02829-17

    authors: Leon IM,Lawhon SD,Norman KN,Threadgill DS,Ohta N,Vinasco J,Scott HM

    更新日期:2018-06-18 00:00:00

  • Appraisal of media and methods for assay of bacteriophages of lactic streptococci.

    abstract::To assess the relative merits of tryptone yeast extract agar, the same medium unbuffered, and medium M17 for the assay of nine bacteriophages of lactic streptococci, comparative plaque counts were made with an overlay of 3 or 9 ml. Four of the phages exhibited no significant difference in plating efficiency between me...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.4.798-802.1980

    authors: Keogh BP

    更新日期:1980-10-01 00:00:00

  • Genetic and symbiotic diversity of nitrogen-fixing bacteria isolated from agricultural soils in the western Amazon by using cowpea as the trap plant.

    abstract::Cowpea is a legume of great agronomic importance that establishes symbiotic relationships with nitrogen-fixing bacteria. However, little is known about the genetic and symbiotic diversity of these bacteria in distinct ecosystems. Our study evaluated the genetic diversity and symbiotic efficiencies of 119 bacterial str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01303-12

    authors: Azarias Guimarães A,Duque Jaramillo PM,Simão Abrahão Nóbrega R,Florentino LA,Barroso Silva K,de Souza Moreira FM

    更新日期:2012-09-01 00:00:00

  • Microbial incidence in upper respiratory tracts of workers in the paper industry.

    abstract::The incidence of microbes in the nasal cavities of workers in three paper and board mills was investigated. A total of 234 persons exposed to microbial aerosols and splashes from paper machine wires and debarker drums formed the exposed group. The control group consisted of 294 workers from the dry working areas: the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.1.163-168.1985

    authors: Niemelä SI,Väätänen P,Mentu J,Jokinen A,Jäppinen P,Sillanpää P

    更新日期:1985-07-01 00:00:00

  • Next-Generation Sequencing Confirms Presumed Nosocomial Transmission of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in the Netherlands.

    abstract:UNLABELLED:Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) was detected in 2003 and rapidly became the predominant MRSA clade in the Netherlands. Studies have shown that transmissions are difficult to identify, since this MRSA variant represents a genetically homogenous clade when current typ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00773-16

    authors: Bosch T,Witteveen S,Haenen A,Landman F,Schouls LM

    更新日期:2016-06-30 00:00:00

  • Improved recovery of Bacillus spores from nonporous surfaces with cotton swabs over foam, nylon, or polyester, and the role of hydrophilicity of cotton in governing the recovery efficiency.

    abstract::Evaluating different swabbing materials for spore recovery efficiency (RE) from steel surfaces, we recorded the maximum RE (71%) of 10(7) Bacillus subtilis spores with Tulips cotton buds, followed by Johnson's cotton buds and standard Hi-Media cotton, polyester, nylon, and foam (23%) swabs. Among cotton swabs, instant...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02626-12

    authors: Thomas P,Mujawar MM,Upreti R,Sekhar AC

    更新日期:2013-01-01 00:00:00

  • Expression of the laccase gene from a white rot fungus in Pichia pastoris can enhance the resistance of this yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system.

    abstract::Laccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00218-12

    authors: Yang Y,Fan F,Zhuo R,Ma F,Gong Y,Wan X,Jiang M,Zhang X

    更新日期:2012-08-01 00:00:00

  • Biocontrol of Listeria monocytogenes and Escherichia coli O157:H7 in meat by using phages immobilized on modified cellulose membranes.

    abstract::The ability of phages to specifically interact with and lyse their host bacteria makes them ideal antibacterial agents. The range of applications of bacteriophage can be extended by their immobilization on inert surfaces. A novel method for the oriented immobilization of bacteriophage has been developed. The method wa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05493-11

    authors: Anany H,Chen W,Pelton R,Griffiths MW

    更新日期:2011-09-01 00:00:00