Abstract:
:An NAD(P)H-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of Arthrobacter nicotinovorans. Transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional activator PmfR and thus was part of the nicotine regulon of A. nicotinovorans. The flavin mononucleotide-containing enzyme uses NADH and, with lower efficiency, NADPH to reduce, by a two-electron transfer, nicotine blue to the nicotine blue leuco form (hydroquinone). Besides nicotine blue, several other quinones were reduced by the enzyme. The NAD(P)H-nicotine blue oxidoreductase may prevent intracellular one-electron reductions of nicotine blue which may lead to semiquinone radicals and potentially toxic reactive oxygen species.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Mihasan M,Chiribau CB,Friedrich T,Artenie V,Brandsch Rdoi
10.1128/AEM.02668-06subject
Has Abstractpub_date
2007-04-01 00:00:00pages
2479-85issue
8eissn
0099-2240issn
1098-5336pii
AEM.02668-06journal_volume
73pub_type
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