Additional evidence that juvenile oyster disease is caused by a member of the Roseobacter group and colonization of nonaffected animals by Stappia stellulata-like strains.

Abstract:

:Juvenile oyster disease (JOD) causes significant annual mortalities of hatchery-produced Eastern oysters, Crassostrea virginica, cultured in the Northeast. We have reported that a novel species of the alpha-proteobacteria Roseobacter group (designated CVSP) was numerically dominant in JOD-affected animals sampled during the 1997 epizootic on the Damariscotta River, Maine. In this study we report the isolation of CVSP bacteria from JOD-affected oysters during three separate epizootics in 1998. These bacteria were not detected in nonaffected oysters at the enzootic site, nor in animals raised at a JOD-free site. Animals raised at the JOD enzootic site that were unaffected by JOD were stably and persistently colonized by Stappia stellulata-like strains. These isolates (designated M1) inhibited the growth of CVSP bacteria in a disk-diffusion assay and thus may have prevented colonization of these animals by CVSP bacteria in situ. Laboratory-maintained C. virginica injected with CVSP bacteria experienced statistically significant elevated mortalities compared to controls, and CVSP bacteria were recovered from these animals during the mortality events. Together, these results provide additional evidence that CVSP bacteria are the etiological agent of JOD. Further, there are no other descriptions of specific marine alpha-proteobacteria that have been successfully cultivated from a defined animal host. Thus, this system presents an opportunity to investigate both bacterial and host factors involved in the establishment of such associations and the role of the invertebrate host in the ecology of these marine alpha-proteobacteria.

journal_name

Appl Environ Microbiol

authors

Boettcher KJ,Barber BJ,Singer JT

doi

10.1128/aem.66.9.3924-3930.2000

keywords:

subject

Has Abstract

pub_date

2000-09-01 00:00:00

pages

3924-30

issue

9

eissn

0099-2240

issn

1098-5336

journal_volume

66

pub_type

杂志文章
  • Influence of Veratryl Alcohol and Hydrogen Peroxide on Ligninase Activity and Ligninase Production by Phanerochaete chrysosporium.

    abstract::Veratryl alcohol, added as a supplement to cultures of Phanerochaete chrysosporium, enhanced ligninase activity through protection of the ligninase against inactivation by hydrogen peroxide produced by this fungus in cultures. In the presence of veratryl alcohol, the loss of ligninase activity observed in non-protein-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.466-472.1988

    authors: Tonon F,Odier E

    更新日期:1988-02-01 00:00:00

  • AcrR and Rex Control Mannitol and Sorbitol Utilization through Their Cross-Regulation of Aldehyde-Alcohol Dehydrogenase (AdhE) in Lactobacillus plantarum.

    abstract::Lactobacillus plantarum is a versatile bacterium that occupies a wide range of environmental niches. In this study, we found that a bifunctional aldehyde-alcohol dehydrogenase-encoding gene, adhE, was responsible for L. plantarum being able to utilize mannitol and sorbitol through cross-regulation by two DNA-binding r...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02035-18

    authors: Yang X,Teng K,Su R,Li L,Zhang T,Fan K,Zhang J,Zhong J

    更新日期:2019-02-06 00:00:00

  • Detection of Listeria monocytogenes in cheese with the magnetic immuno-polymerase chain reaction assay.

    abstract::A new detection system, the magnetic immuno-polymerase chain reaction (PCR) assay (MIPA) has been developed to detect Listeria monocytogenes in food. This method separates Listeria cells from PCR-inhibitory factors present in enrichment broths containing food samples by using magnetic beads coated with specific monocl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.5.1289-1293.1993

    authors: Fluit AC,Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,Keller BH,Klapwijk P,Verhoef J

    更新日期:1993-05-01 00:00:00

  • The Hydroxyquinol Degradation Pathway in Rhodococcus jostii RHA1 and Agrobacterium Species Is an Alternative Pathway for Degradation of Protocatechuic Acid and Lignin Fragments.

    abstract::Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01561-20

    authors: Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

    更新日期:2020-09-17 00:00:00

  • Purification and Characterization of an Aminopeptidase from Lactococcus lactis subsp. cremoris AM2.

    abstract::An aminopeptidase was purified from cell extracts of Lactococcus lactis subsp. cremoris AM2 by ion-exchange chromatography. After electrophoresis of the purified enzyme in the presence or absence of sodium dodecyl sulfate, one protein band was detected. The enzyme was a 300-kilodalton hexamer composed of identical sub...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.9.2308-2314.1989

    authors: Neviani E,Boquien CY,Monnet V,Thanh LP,Gripon JC

    更新日期:1989-09-01 00:00:00

  • Sequence variability of P2-like prophage genomes carrying the cytolethal distending toxin V operon in Escherichia coli O157.

    abstract::Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01134-13

    authors: Sváb D,Horváth B,Maróti G,Dobrindt U,Tóth I

    更新日期:2013-08-01 00:00:00

  • Evidence for iron-dependent nitrate respiration in the dissimilatory iron-reducing bacterium Geobacter metallireducens.

    abstract::The dissimilatory iron-reducing bacterium Geobacter metallireducens was found to require iron at a concentration in excess of 50 microM for continuous cultivation on nitrate. Growth yield (approximately 3-fold), cytochrome c content (approximately 7-fold), and nitrate (approximately 4.5-fold) and nitrite (approximatel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.8.3750-3752.2001

    authors: Senko JM,Stolz JF

    更新日期:2001-08-01 00:00:00

  • Transcriptomic insights into the physiology of Aspergillus niger approaching a specific growth rate of zero.

    abstract::The physiology of filamentous fungi at growth rates approaching zero has been subject to limited study and exploitation. With the aim of uncoupling product formation from growth, we have revisited and improved the retentostat cultivation method for Aspergillus niger. A new retention device was designed allowing reliab...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00450-10

    authors: Jørgensen TR,Nitsche BM,Lamers GE,Arentshorst M,van den Hondel CA,Ram AF

    更新日期:2010-08-01 00:00:00

  • Contributions of Autotrophic and Heterotrophic Nitrifiers to Soil NO and N(2)O Emissions.

    abstract::Soil emission of gaseous N oxides during nitrification of ammonium represents loss of an available plant nutrient and has an important impact on the chemistry of the atmosphere. We used selective inhibitors and a glucose amendment in a factorial design to determine the relative contributions of autotrophic ammonium ox...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.6.1799-1805.1990

    authors: Tortoso AC,Hutchinson GL

    更新日期:1990-06-01 00:00:00

  • Expression and functional analysis of a hyperglycosylated glucoamylase in a parental host, Aspergillus awamori var. kawachi.

    abstract::A modified glucoamylase gene (glaA) with an extra Thr- and Ser-rich Gp-I domain (T. Semimaru, M. Goto, K. Furukawa, and S. Hayashida, Appl. Environ. Microbiol. 61:2885-2890, 1995) was introduced into a mutant parental host, Aspergillus awamori var. kawachi, in which the original glaA gene had been completely deleted a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.7.2940-2943.1997

    authors: Goto M,Ekino K,Furukawa K

    更新日期:1997-07-01 00:00:00

  • Bacteria on closed-boll and commercially harvested cotton.

    abstract::The bacterial content of specially treated cottons used by other investigators to test human pulmonary responses to cotton dust was examined. Cotton from Lubbock, Tex. and Stoneville, Miss. were either (i) harvested by machine and handled as commercial bale cotton, (ii) harvested as closed bolls with bracts intact and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.2.355-362.1982

    authors: Millner PD,Ericson KE,Marsh PB

    更新日期:1982-08-01 00:00:00

  • Characterization of a Small RNA-Containing Virus in Field-Collected Larvae of the Tussock Moth, Lymantria ninayi, from Papua New Guinea.

    abstract::Field-collected larvae of the tussock moth, Lymantria ninayi, a major pest of exotic pines in Papua New Guinea, were found to contain a small RNA-containing virus with a diameter of 29 nm and a buoyant density of 1.32 g/ml. The RNA was single stranded, had a molecular weight of 2.8 x 10, and was polyadenylated. Virion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.3.504-507.1984

    authors: Pullin JS,Black F,King LA,Entwistle PF,Moore NF

    更新日期:1984-09-01 00:00:00

  • Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.

    abstract::Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolera...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00018-19

    authors: Booker AE,Hoyt DW,Meulia T,Eder E,Nicora CD,Purvine SO,Daly RA,Moore JD,Wunch K,Pfiffner SM,Lipton MS,Mouser PJ,Wrighton KC,Wilkins MJ

    更新日期:2019-05-30 00:00:00

  • Production and characterization of antibodies against microcystins.

    abstract::Antibodies against a microcystin (MCYST) leucine-arginine variant (MCYST-LR) were demonstrated 4 weeks after immunization of rabbits with either MCYST-LR-polylysine- or MCYST-LR-ethylenediamine-modified bovine serum albumin. A radioimmunoassay (RIA), a direct competitive enzyme-linked immunosorbent assay (ELISA), and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.1928-1933.1989

    authors: Chu FS,Huang X,Wei RD,Carmichael WW

    更新日期:1989-08-01 00:00:00

  • Isolation of amoebae from edible mushrooms.

    abstract::Isolation of amoebae from the surface and internal tissues of edible mushrooms was investigated. Samples tested over a 3-year period included mushrooms cultivated from six geographic localities. Of 168 mushroom surfaces tested, 161 (96%) yielded amoebae. Of 166 samples of internal stalk and cap tissues tested, only 1 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.1.255-257.1982

    authors: Napolitano JJ

    更新日期:1982-07-01 00:00:00

  • N-demethylation of nicotine and reduction of nicotine-1'-N-oxide by Microsporum gypseum.

    abstract::Several microorganisms were examined for their abilities to convert S-nicotine into nornicotine. Five microorganisms including Microsporum gypseum (ATCC 11395) produced nornicotine and three unknown metabolites. M. gypseum efficiently reduced nicotine-1'-N-oxide to nicotine, but no nornicotine was obtained when the N-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.38.5.836-839.1979

    authors: Sindelar RD,Rosazza JP,Barfknecht CF

    更新日期:1979-11-01 00:00:00

  • Metabolism of Linoleic Acid or Mevalonate and 6-Pentyl-alpha-Pyrone Biosynthesis by Trichoderma Species.

    abstract::The understanding of the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U-C]linoleic acid or [5-C]sodium mevalonate revealed that both fungal strains...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.9.2945-2950.1993

    authors: Serrano-Carreon L,Hathout Y,Bensoussan M,Belin JM

    更新日期:1993-09-01 00:00:00

  • Chemical Composition of the Edible Mushroom Pleurotus ostreatus Produced by Fermentation.

    abstract::Pleurotus ostreatus ;Florida' was grown in submerged liquid culture. The biomass yield of the fungus, grown for 3 days in 2-liter fermentors, where the mycelial pellets measuring 5 mm in diameter were formed, was 11.7 g (dry weight)/liter. Comparing the chemical constituents of fruiting bodies produced on cotton straw...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.6.1352-1354.1986

    authors: Hadar Y,Cohen-Arazi E

    更新日期:1986-06-01 00:00:00

  • Diversity of the Sediment Microbial Community in the Aha Watershed (Southwest China) in Response to Acid Mine Drainage Pollution Gradients.

    abstract::Located in southwest China, the Aha watershed is continually contaminated by acid mine drainage (AMD) produced from upstream abandoned coal mines. The watershed is fed by creeks with elevated concentrations of aqueous Fe (total Fe > 1 g/liter) and SO4 (2-) (>6 g/liter). AMD contamination gradually decreases throughout...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00935-15

    authors: Sun W,Xiao T,Sun M,Dong Y,Ning Z,Xiao E,Tang S,Li J

    更新日期:2015-08-01 00:00:00

  • Toxicity of 1,1,1-Trichloroethane and Trichloroethene on a Mixed Culture of Methane-Oxidizing Bacteria.

    abstract::The influence of trichloroethene (TCE; 0 to 65 mg/liter) and 1,1,1-trichloroethane (1,1,1-TCA; 0 to 103 mg/liter) on methane consumption of a mixed culture of methane-oxidizing bacteria was studied in laboratory batch experiments. Increasing concentrations of TCE or 1,1,1-TCA resulted in decreasing methane consumption...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.8.2488-2493.1990

    authors: Broholm K,Jensen BK,Christensen TH,Olsen L

    更新日期:1990-08-01 00:00:00

  • Evidence for coexistence of distinct Escherichia coli populations in various aquatic environments and their survival in estuary water.

    abstract::Escherichia coli, a commensal bacterium from the intestinal tracts of humans and vertebrate animals, has been used as one of two bacterial indicators of fecal contamination, along with intestinal enterococci, to monitor the microbiological quality of water. However, water environments are now recognized as a secondary...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00698-13

    authors: Berthe T,Ratajczak M,Clermont O,Denamur E,Petit F

    更新日期:2013-08-01 00:00:00

  • Changes in Endoglucanase Patterns during Growth of Thermomonospora curvata on Cellulose.

    abstract::The endoglucanases of the thermophilic actinomycete Thermomonospora curvata were characterized. Early-exponential-phase culture fluid contained at least three endoglucanases, with molecular weights of 23,000, 46,000, and 146,000 and K(m) values of 1.54, 3.60, and 1.32% carboxymethyl cellulose, respectively. The statio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.2.588-589.1988

    authors: Lupo D,Stutzenberger F

    更新日期:1988-02-01 00:00:00

  • Alternative Sigma Factor RpoX Is a Part of the RpoE Regulon and Plays Distinct Roles in Stress Responses, Motility, Biofilm Formation, and Hemolytic Activities in the Marine Pathogen Vibrio alginolyticus.

    abstract::Vibrio alginolyticus is one of the most abundant microorganisms in marine environments and is also an opportunistic pathogen mediating high-mortality vibriosis in marine animals. Alternative sigma factors play essential roles in bacterial pathogens in the adaptation to environmental changes during infection and the ad...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00234-19

    authors: Gu D,Zhang J,Hao Y,Xu R,Zhang Y,Ma Y,Wang Q

    更新日期:2019-07-01 00:00:00

  • Possible waterborne transmission and maintenance of influenza viruses in domestic ducks.

    abstract::Two duck farms in Hong Kong were examined monthly for 1 year for the occurrence and persistence of influenza viruses within the duck communities. The predominant virus in one community was H3N2, a virus antigenically related to the pandemic Hong Kong strain. This virus was isolated monthly throughout the year from fec...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.1.110-115.1982

    authors: Markwell DD,Shortridge KF

    更新日期:1982-01-01 00:00:00

  • Improvement of xylose uptake and ethanol production in recombinant Saccharomyces cerevisiae through an inverse metabolic engineering approach.

    abstract::We used an inverse metabolic engineering approach to identify gene targets for improved xylose assimilation in recombinant Saccharomyces cerevisiae. Specifically, we created a genomic fragment library from Pichia stipitis and introduced it into recombinant S. cerevisiae expressing XYL1 and XYL2. Through serial subcult...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8249-8256.2005

    authors: Jin YS,Alper H,Yang YT,Stephanopoulos G

    更新日期:2005-12-01 00:00:00

  • A group II intron-type open reading frame from the thermophile Bacillus (Geobacillus) stearothermophilus encodes a heat-stable reverse transcriptase.

    abstract::The production of a stable cDNA copy of an unstable RNA molecule by reverse transcription is a widely used and essential technology for many important applications, such as the construction of gene libraries, production of DNA probes, and analysis of gene expression by reverse transcriptase PCR (RT-PCR). However, the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.7140-7147.2004

    authors: Vellore J,Moretz SE,Lampson BC

    更新日期:2004-12-01 00:00:00

  • Quantitative detection of perchlorate-reducing bacteria by real-time PCR targeting the perchlorate reductase gene.

    abstract::A quantitative real-time PCR assay targeting the pcrA gene, encoding the catalytic subunit of perchlorate reductase, detected pcrA genes from perchlorate-reducing bacteria in three different genera and from soil microbial communities. Partial pcrA sequences indicated differences in the composition of perchlorate-reduc...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01658-07

    authors: Nozawa-Inoue M,Jien M,Hamilton NS,Stewart V,Scow KM,Hristova KR

    更新日期:2008-03-01 00:00:00

  • Spatial and temporal variation of phenanthrene-degrading bacteria in intertidal sediments.

    abstract::Phenanthrene-degrading bacteria were isolated from a 1-m2 intertidal sediment site in Boston Harbor. Samples were taken six times over 2 years. A total of 432 bacteria were isolated and characterized by biochemical testing. When clustered on the basis of phenotypic characteristics, the isolates could be separated into...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.7.2560-2565.1998

    authors: Berardesco G,Dyhrman S,Gallagher E,Shiaris MP

    更新日期:1998-07-01 00:00:00

  • Phomenoic acid and phomenolactone, antifungal substances from Phoma lingam (Tode) Desm.: kinetics of their biosynthesis, with an optimization of the isolation procedures.

    abstract::Phomenoic acid and phomenolactone, previously isolated from the fungus Phoma lingam (Tode) Desm., have shown moderate antifungal and antibacterial properties in vitro. To rationalize the production of phomenoic acid, a kinetic study of its biosynthesis in the mycelium was performed. Phomenoic acid and phomenolactone a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.5.966-968.1987

    authors: Topgi RS,Devys M,Bousquet JF,Kollmann A,Barbier M

    更新日期:1987-05-01 00:00:00

  • Identification of Streptomyces coelicolor proteins that are differentially expressed in the presence of plant material.

    abstract::Streptomyces coelicolor and Lemna minor were used as a model to study the modulation of bacterial gene expression during plant-streptomycete interactions. S. coelicolor was grown in minimal medium with and without L. minor fronds. Bacterial proteomes were analyzed by two-dimensional gel electrophoresis, and a comparis...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.4.1884-1889.2003

    authors: Langlois P,Bourassa S,Poirier GG,Beaulieu C

    更新日期:2003-04-01 00:00:00