Abstract:
:The understanding of the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species was achieved by using labelled linoleic acid or mevalonate as a tracer. Incubation of growing cultures of Trichoderma harzianum and T. viride with [U-C]linoleic acid or [5-C]sodium mevalonate revealed that both fungal strains were able to incorporate these labelled compounds (50 and 15%, respectively). Most intracellular radioactivity was found in the neutral lipid fraction. At the initial time of incubation, the radioactivity from [C]linoleic acid was incorporated into 6-pentyl-alpha-pyrone more rapidly than that from [C]mevalonate. No radioactivity incorporation was detected in 6-pentyl-alpha-pyrone when fungal cultures were incubated with [1-C]linoleic acid. These results suggested that beta-oxidation of linoleic acid was a probable main step in the biosynthetic pathway of 6-pentyl-alpha-pyrone in Trichoderma species.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Serrano-Carreon L,Hathout Y,Bensoussan M,Belin JMdoi
10.1128/AEM.59.9.2945-2950.1993keywords:
subject
Has Abstractpub_date
1993-09-01 00:00:00pages
2945-50issue
9eissn
0099-2240issn
1098-5336journal_volume
59pub_type
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