Engineering cytochrome P450 BM-3 for oxidation of polycyclic aromatic hydrocarbons.

abstract：:Fecal droppings of migratory Canada geese, Branta canadensis, collected from nine sites near the Chesapeake Bay (Maryland), were examined for the presence of Cryptosporidium parvum and Giardia spp. Cryptosporidium sp. oocysts were found in feces at seven of nine sites, and Giardia cysts were found at all nine sites. T...

journal_title：Applied and environmental microbiology

authors： Graczyk TK,Fayer R,Trout JM,Lewis EJ,Farley CA,Sulaiman I,Lal AA

更新日期：1998-07-01 00:00:00

abstract：:Rotavirus SA11, suspended in tryptose phosphate broth with 2.5 mg of rhodamine B per ml, was aerosolized (Collison nebulizer) into a rotating drum, and the aerosols were held at 20 +/- 1 degree C with the desired relative humidity (RH). An all-glass impinger with tryptose phosphate broth was used to collect 1-min (5.6...

journal_title：Applied and environmental microbiology

authors： Sattar SA,Ijaz MK,Johnson-Lussenburg CM,Springthorpe VS

更新日期：1984-04-01 00:00:00

abstract：:The mercury resistance (mer) operon of plasmid R100 was cloned onto various plasmid vectors to study the effect of mer gene amplification on the rate of Hg2+ reduction by Escherichia coli cells. The plasmids were maintained at copy numbers ranging from 3 to 140 copies per cell. The overall Hg2+ reduction rate of intac...

journal_title：Applied and environmental microbiology

authors： Philippidis GP,Malmberg LH,Hu WS,Schottel JL

更新日期：1991-12-01 00:00:00

abstract：:A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...

journal_title：Applied and environmental microbiology

authors： van de Guchte M,van der Vossen JM,Kok J,Venema G

更新日期：1989-01-01 00:00:00

abstract：:The aim of this work was to develop a strategy to isolate a morphologically stable mutant of Streptomyces ambofaciens ATCC 15154 which produced high titers of spiramycin. The rationale was to grow a nitrosoguanidine-mutated population for many generations under nonselective conditions followed by two cycles of protopl...

journal_title：Applied and environmental microbiology

authors： Ford LM,Eaton TE,Godfrey OW

更新日期：1990-11-01 00:00:00

abstract：:We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...

journal_title：Applied and environmental microbiology

authors： Liu J,Xie Z,Merritt J,Qi F

更新日期：2012-05-01 00:00:00

abstract：:We have used a sintered glass bead core to simulate the spaces and surfaces of reservoir rock in studies of the bacterial plugging phenomenon that affects waterflood oil recovery operations. The passage of pure or mixed natural populations of bacteria through this solid matrix was initially seen to promote the formati...

journal_title：Applied and environmental microbiology

authors： Shaw JC,Bramhill B,Wardlaw NC,Costerton JW

更新日期：1985-03-01 00:00:00

abstract：:Bacteria play important roles in mineral weathering and soil formation. However, few reports of mineral weathering bacteria inhabiting subsurfaces of soil profiles have been published, raising the question of whether the subsurface weathering bacteria are fundamentally distinct from those in surface communities. To ad...

journal_title：Applied and environmental microbiology

authors： Huang J,Sheng XF,Xi J,He LY,Huang Z,Wang Q,Zhang ZD

更新日期：2014-01-01 00:00:00

abstract：:Of 199 fungal cultures isolated from some of the common cereals collected from different parts of Uttar Pradesh and Madhya Pradesh, 70 produced toxic metabolites. Of the 70 fungi isolated, 59 produced toxins which caused visible lesions in livers, kidneys, and spleens but did not cause mortality. Toxicity, graded in t...

journal_title：Applied and environmental microbiology

authors： Gupta J,Pathak B,Sethi N,Vora VC

更新日期：1981-03-01 00:00:00

abstract：:Treatment of secondary effluents with hydrogen peroxide (10 mg/liter)-ascorbic acid (10 mg/liter)-Cu2+ (0.5 mg/liter) for 60 min resulted in around 99% reduction of the initial plate count. Hydrogen peroxide could be replaced by other peroxygen compounds; ascorbic acid could be replaced by other reducing agents, of wh...

journal_title：Applied and environmental microbiology

authors： Ragab-Depre NJ

更新日期：1982-09-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:Wastewater management in the Canadian Arctic is challenging due to climate extremes, small population sizes, and lack of conventional infrastructure for wastewater treatment. Although many Northern communities use waste stabilization ponds (WSPs) as their primary form of wastewater treatment, few studies have explored...

journal_title：Applied and environmental microbiology

authors： Gromala M,Neufeld JD,McConkey BJ

更新日期：2021-01-15 00:00:00

abstract：:The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the major ribotypes. DGGE profiles of the...

journal_title：Applied and environmental microbiology

authors： Favier CF,Vaughan EE,De Vos WM,Akkermans AD

更新日期：2002-01-01 00:00:00

abstract：:The rapid degradation of marine infrastructure at the low tide level due to accelerated low-water corrosion (ALWC) is a problem encountered worldwide. Despite this, there is limited understanding of the microbial communities involved in this process. We obtained samples of the orange-colored tubercles commonly associa...

journal_title：Applied and environmental microbiology

authors： Phan HC,Wade SA,Blackall LL

更新日期：2020-06-17 00:00:00

abstract：:Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

journal_title：Applied and environmental microbiology

authors： Cluss RG,Johnson JK,Somerson NL

更新日期：1983-08-01 00:00:00

abstract：:Caldalkalibacillus thermarum strain TA2.A1 grew in pH-controlled batch culture containing a fermentable growth substrate (i.e., sucrose) from pH 7.5 to 10.0 with no significant change in the specific growth rate, suggesting that this bacterium was a facultative alkaliphile. However, when strain TA2.A1 was grown on a n...

journal_title：Applied and environmental microbiology

authors： McMillan DG,Keis S,Berney M,Cook GM

更新日期：2009-12-01 00:00:00

abstract：:Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine. ...

journal_title：Applied and environmental microbiology

authors： Varon M,Seijffers J

更新日期：1977-05-01 00:00:00

abstract：:Bacteroides fragilis subsp. fragilis was maintained in a chemostat modified for anaerobic conditions to test the effects of dissolved oxygen and Eh on growth. Using a defined medium containing glucose and a dilution rate of 0.16 h -1, a stable population of 3 X 10(9) colony-forming units/ml was present. At this steady...

journal_title：Applied and environmental microbiology

authors： Onderdonk AB,Johnston J,Mayhew JW,Gorbach SL

更新日期：1976-02-01 00:00:00

abstract：:The presence and chain length of mycolic acids of bacteria of the genera Corynebacterium, Rhodococcus, Gordona, Mycobacterium, and Arthrobacter and of coryneform bacteria containing a type B peptidoglycan were related to the cell surface hydrophobicity of the bacteria, which in turn was related to adhesion of the cell...

journal_title：Applied and environmental microbiology

authors： Bendinger B,Rijnaarts HH,Altendorf K,Zehnder AJ

更新日期：1993-11-01 00:00:00

abstract：:A cellulosome-microbe complex was assembled ex vivo on the surface of Bacillus subtilis displaying a miniscaffoldin that can bind with three dockerin-containing cellulase components: the endoglucanase Cel5, the processive endoglucanase Cel9, and the cellobiohydrolase Cel48. The hydrolysis performances of the synthetic...

journal_title：Applied and environmental microbiology

authors： You C,Zhang XZ,Sathitsuksanoh N,Lynd LR,Zhang YH

更新日期：2012-03-01 00:00:00

abstract：:The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...

journal_title：Applied and environmental microbiology

authors： Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

更新日期：2004-08-01 00:00:00

abstract：:Tellurium, a metalloid belonging to group 16 of the periodic table, displays very interesting physical and chemical properties and lately has attracted significant attention for its use in nanotechnology. In this context, the use of microorganisms for synthesizing nanostructures emerges as an eco-friendly and exciting...

journal_title：Applied and environmental microbiology

authors： Pugin B,Cornejo FA,Muñoz-Díaz P,Muñoz-Villagrán CM,Vargas-Pérez JI,Arenas FA,Vásquez CC

更新日期：2014-11-01 00:00:00

abstract：:Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

journal_title：Applied and environmental microbiology

authors： Ahonen L,Tuovinen OH

更新日期：1990-02-01 00:00:00

abstract：:Some nitrate- and Fe(III)-reducing microorganisms are capable of oxidizing Fe(II) with nitrate as the electron acceptor. This enzymatic pathway may facilitate the development of anaerobic microbial communities that take advantage of the energy available during Fe-N redox oscillations. We examined this phenomenon in sy...

journal_title：Applied and environmental microbiology

authors： Coby AJ,Picardal F,Shelobolina E,Xu H,Roden EE

更新日期：2011-09-01 00:00:00

abstract：:Parental strains and asporogenous mutants of Bacillus thuringiensis subspp. kurstaki and aizawai produced high yields of delta-endotoxin on M medium, which contained 330 mug of potassium per ml, but not on ST and ST-a media, each of which contained only 11 mug of potassium per ml. On ST and ST-a media, refractile gran...

journal_title：Applied and environmental microbiology

authors： Wakisaka Y,Masaki E,Nishimoto Y

更新日期：1982-06-01 00:00:00

abstract：:Unlike general peroxidases, Pleurotus ostreatus MnP2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as Poly R-478 and RNase A. To elucidate the mechanism for oxidation of polymeric substrates by MnP2, a series of mutant enzymes were produced by using a homologous ...

journal_title：Applied and environmental microbiology

authors： Tsukihara T,Honda Y,Sakai R,Watanabe T,Watanabe T

更新日期：2008-05-01 00:00:00

abstract：:Two thermostable lipases were isolated and characterized from Thermosyntropha lipolytica DSM 11003, an anaerobic, thermophilic, alkali-tolerant bacterium which grows syntrophically with methanogens on lipids such as olive oil, utilizing only the liberated fatty acid moieties but not the glycerol. Lipases LipA and LipB...

journal_title：Applied and environmental microbiology

authors： Salameh MA,Wiegel J

更新日期：2007-12-01 00:00:00

abstract：:The distribution of Borrelia burgdorferi sensu lato genospecies in questing Ixodes ricinus ticks from ecologically distinct habitats in Latvia was analyzed. A significant variation in the frequency of the genospecies across sites was observed, pointing to the importance of the host community in the ecology of Lyme bor...

journal_title：Applied and environmental microbiology

authors： Etti S,Hails R,Schäfer SM,De Michelis S,Sewell HS,Bormane A,Donaghy M,Kurtenbach K

更新日期：2003-05-01 00:00:00

abstract：:The induction of the inducible lysyl-tRNA synthetase, LysU, and the inducible lysine and arginine decarboxylases of Escherichia coli K-12 grown in AC broth to a pH of 5.5 or less is temperature dependent, being distinctly lower at 24 than at 37 degrees C. This induction does not appear to be under HtpR control. ...

journal_title：Applied and environmental microbiology

authors： Hassani M,Pincus DH,Bennett GN,Hirshfield IN

更新日期：1992-08-01 00:00:00

abstract：:The filamentous fungus Aspergillus fumigatus normally grows on compost or hay but is also able to colonize environments such as the human lung. In order to survive, this organism needs to react to a multitude of external stimuli. Although extensive work has been carried out to investigate intracellular signal transduc...

journal_title：Applied and environmental microbiology

authors： Gehrke A,Heinekamp T,Jacobsen ID,Brakhage AA

更新日期：2010-06-01 00:00:00