Establishment of a tractable genetic transformation system in Veillonella spp.

abstract：:A diesel fuel-contaminated aquifer was bioremediated in situ by the injection of oxidants (O2 and NO3-) and nutrients in order to stimulate microbial activity. After 3.5 years of remediation, an aquifer sample was excavated and the material was used (i) to isolate bacterial strains able to grow on selected hydrocarbon...

journal_title：Applied and environmental microbiology

authors： Hess A,Zarda B,Hahn D,Häner A,Stax D,Höhener P,Zeyer J

更新日期：1997-06-01 00:00:00

abstract：:Reservoir 29 and Lake B are extremely acid lakes (epilimnion pHs of 2.7 and 3.2, respectively), because they receive acidic discharges from coal refuse piles. They differ in that the pH of profundal sediments in Reservoir 29 increased from 2.7 to 3.8 during the period of thermal stratification, whereas permanently ano...

journal_title：Applied and environmental microbiology

authors： Gyure RA,Konopka A,Brooks A,Doemel W

更新日期：1987-09-01 00:00:00

abstract：:Many insects are associated with heritable symbionts that mediate ecological interactions, including host protection against natural enemies. The cowpea aphid, Aphis craccivora, is a polyphagous pest that harbors Hamiltonella defensa, which defends against parasitic wasps. Despite this protective benefit, this symbion...

journal_title：Applied and environmental microbiology

authors： Dykstra HR,Weldon SR,Martinez AJ,White JA,Hopper KR,Heimpel GE,Asplen MK,Oliver KM

更新日期：2014-09-01 00:00:00

abstract：:Under anoxic conditions, most methoxylated mononuclear aromatic compounds are degraded by bacteria, with catechol being formed as an important intermediate. On the basis of our experiments with the sulfate-reducing bacterium Desulfobacterium sp. strain Cat2, we describe for the first time the enzymatic activities invo...

journal_title：Applied and environmental microbiology

authors： Gorny N,Schink B

更新日期：1994-09-01 00:00:00

abstract：:Current approaches used for dose-response modeling of low-dose exposures of pathogens rely on assumptions and extrapolations. These models are important for quantitative microbial risk assessment of food. A mechanistic framework has been advocated as an alternative approach for evaluating dose-response relationships. ...

journal_title：Applied and environmental microbiology

authors： Abe H,Koyama K,Koseki S

更新日期：2020-12-17 00:00:00

abstract：:The role of protease produced by a heterotrophic bacterium during growth was investigated with Aeromonas salmonicida, the pathogen of fish furunculosis, strain A-7301 and its protease-deficient mutant NTG-1 induced by mutagenesis. Strain A-7301 produced extracellular protease in a mixed amino acid medium (composed of ...

journal_title：Applied and environmental microbiology

authors： Sakai DK

更新日期：1985-10-01 00:00:00

abstract：:Pseudomonas syringae is a common foliar bacterium responsible for many important plant diseases. We studied the population structure and dynamics of the core genome of P. syringae via multilocus sequencing typing (MLST) of 60 strains, representing 21 pathovars and 2 nonpathogens, isolated from a variety of plant hosts...

journal_title：Applied and environmental microbiology

authors： Sarkar SF,Guttman DS

更新日期：2004-04-01 00:00:00

abstract：:This paper explores the relationship between the genetic diversity of rhizobia and the morphological diversity of their plant hosts. Rhizobium galegae strains were isolated from nodules of wild Galega orientalis and Galega officinalis in the Caucasus, the center of origin for G. orientalis. All 101 isolates were chara...

journal_title：Applied and environmental microbiology

authors： Andronov EE,Terefework Z,Roumiantseva ML,Dzyubenko NI,Onichtchouk OP,Kurchak ON,Dresler-Nurmi A,Young JP,Simarov BV,Lindström K

更新日期：2003-02-01 00:00:00

abstract：:The significance of dissolved combined amino acids (DCAA), dissolved free amino acids (DFAA), and dissolved DNA (D-DNA) as sources of C and N for marine bacteria in batch cultures with variable substrate C/N ratios was studied. Glucose, ammonium, alanine, and phosphate were added to the cultures to produce C/N ratios ...

journal_title：Applied and environmental microbiology

authors： Jørgensen NO,Kroer N,Coffin RB

更新日期：1994-11-01 00:00:00

abstract：:Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins w...

journal_title：Applied and environmental microbiology

authors： Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico F

更新日期：2004-01-01 00:00:00

abstract：:Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

journal_title：Applied and environmental microbiology

authors： Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

更新日期：2011-12-01 00:00:00

abstract：:The presence or absence of molecular oxygen has been shown to play a crucial role in the degradability of haloaromatic compounds. In the present study, it was shown that anaerobic phototrophic 3-chlorobenzoate (3CBA) metabolism by Rhodopseudomonas palustris DCP3 is oxygen tolerant up to a concentration of 3 microM O2....

journal_title：Applied and environmental microbiology

authors： Krooneman J,van den Akker S,Pedro Gomes TM,Forney LJ,Gottschal JC

更新日期：1999-01-01 00:00:00

abstract：:The noncanonical alcohol dehydrogenase AlkJ is encoded on the alkane-metabolizing alk operon of the mesophilic bacterium Pseudomonas putida GPo1. To gain insight into the enzymology of AlkJ, we have produced the recombinant protein in Escherichia coli and purified it to homogeneity using His6 tag affinity and size exc...

journal_title：Applied and environmental microbiology

authors： Kirmair L,Skerra A

更新日期：2014-04-01 00:00:00

abstract：UNLABELLED:Modulation of the intestinal microbial ecosystem (IME) is a useful target to establish probiotic efficacy in a healthy population. We conducted a randomized, double-blind, crossover, and placebo-controlled intervention study to determine the impact of Bifidobacterium bifidum strain Bb on the IME of adult hea...

journal_title：Applied and environmental microbiology

authors： Gargari G,Taverniti V,Balzaretti S,Ferrario C,Gardana C,Simonetti P,Guglielmetti S

更新日期：2016-09-16 00:00:00

abstract：:This paper introduces a novel method for sampling pathogens in natural environments. It uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. Wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). Thi...

journal_title：Applied and environmental microbiology

authors： Jones NR,Millman C,van der Es M,Hukelova M,Forbes KJ,Glover C,Haldenby S,Hunter PR,Jackson K,O'Brien SJ,Rigby D,Strachan NJC,Williams N,Lake IR,ENIGMA Consortium.

更新日期：2017-06-30 00:00:00

abstract：:Although several microorganisms that produce and degrade methanethiol (MT) and dimethyl sulfide (DMS) have been isolated from various habitats, little is known about the numbers of these microorganisms in situ. This study reports on the identification and quantification of microorganisms involved in the cycling of MT ...

journal_title：Applied and environmental microbiology

authors： Lomans BP,Luderer R,Steenbakkers P,Pol A,van Der Drift C,Vogels GD,Op den Camp HJ

更新日期：2001-03-01 00:00:00

abstract：:Cells of the gram-negative bacterium Ralstonia sp. strain SBUG 290 grown in the presence of biphenyl are able to cooxidize dibenzofuran which has been 1,2-hydroxylated. Meta cleavage of the 1, 2-dihydroxydibenzofuran between carbon atoms 1 and 9b produced 2-hydroxy-4-(3'-oxo-3'H-benzofuran-2'-yliden)but-2-enoic acid, ...

journal_title：Applied and environmental microbiology

authors： Becher D,Specht M,Hammer E,Francke W,Schauer F

更新日期：2000-10-01 00:00:00

abstract：:Disulfide bonds are important for the correct folding, structural integrity, and activity of many biotechnologically relevant proteins. For synthesis and subsequent secretion of these proteins in bacteria, such as the well-known "cell factory" Bacillus subtilis, it is often the correct formation of disulfide bonds tha...

journal_title：Applied and environmental microbiology

authors： Kouwen TR,Dubois JY,Freudl R,Quax WJ,van Dijl JM

更新日期：2008-12-01 00:00:00

abstract：:Filamentous, gliding, sulfide-oxidizing bacteria of the genus Thioploca were found on sediments in profundal areas of Lake Biwa, a Japanese freshwater mesotrophic lake, and were characterized morphologically and phylogenetically. The Lake Biwa Thioploca resembled morphologically Thioploca ingrica, a brackish water spe...

journal_title：Applied and environmental microbiology

authors： Kojima H,Teske A,Fukui M

更新日期：2003-01-01 00:00:00

abstract：:A real-time immunoquantitative PCR (iqPCR) method for detection of Staphylococcus aureus enterotoxin B (SEB) was developed and evaluated using both pure cultures and foods. The assay consisted of immunocapture of SEB and real-time PCR amplification of the DNA probe linked to the detection antibody. iqPCR was compared ...

journal_title：Applied and environmental microbiology

authors： Rajkovic A,El-Moualij B,Uyttendaele M,Brolet P,Zorzi W,Heinen E,Foubert E,Debevere J

更新日期：2006-10-01 00:00:00

abstract：:Yarrowia lipolytica produces brown extracellular pigments that correlate with tyrosine catabolism. During tyrosine depletion, the yeast accumulated homogentisic acid, p-hydroxyphenylethanol, and p-hydroxyphenylacetic acid in the medium. Homogentisic acid accumulated under all aeration conditions tested, but its concen...

journal_title：Applied and environmental microbiology

authors： Carreira A,Ferreira LM,Loureiro V

更新日期：2001-08-01 00:00:00

abstract：:Intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. The quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microor...

journal_title：Applied and environmental microbiology

authors： Ercolini D,De Filippis F,La Storia A,Iacono M

更新日期：2012-11-01 00:00:00

abstract：:This research documents an effect of reactor turbulence on the ability of gram-negative wastewater biofilm bacteria to actively transport l-aspartate via a binding-protein-mediated transport system. Biofilms which were not preadapted to turbulence and which possessed two separate and distinct aspartate transport syste...

journal_title：Applied and environmental microbiology

authors： Eighmy TT,Bishop PL

更新日期：1985-07-01 00:00:00

abstract：:A method for the rapid lysis of Listeria cells, employing a recombinant Listeria bacteriophage A118 lytic enzyme (PLY118), is described. The procedure can be used with all listerial species. It enables fast, efficient, and gentle recovery of DNA, RNA, or native cellular proteins from small-scale (2- to 5-ml) cultures....

journal_title：Applied and environmental microbiology

authors： Loessner MJ,Schneider A,Scherer S

更新日期：1995-03-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00

abstract：:Cholesterol and albumin are limiting factors in the growth of Mycoplasma species. These nutrients are usually supplied in the culture medium by the addition of serum. The growth of M. pneumoniae in a serum-free medium containing an ethanolic cholesterol suspension and albumin was about one-half the level attained in s...

journal_title：Applied and environmental microbiology

authors： Cluss RG,Johnson JK,Somerson NL

更新日期：1983-08-01 00:00:00

abstract：:The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

journal_title：Applied and environmental microbiology

authors： Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

更新日期：2007-03-01 00:00:00

abstract：:The effects of Cd2+, Cu2+, Mg2+, and Zn2+ on the decomposition of citric acid by a Klebsiella sp. were studied by monitoring the degradation of [14C]citrate. The carbon concentration used was 10 micrograms of C liter-1, and the media were designed to provide at least 95% of the citrate complexed to the metal studied. ...

journal_title：Applied and environmental microbiology

authors： Brynhildsen L,Rosswall T

更新日期：1989-06-01 00:00:00

abstract：:A novel type II restriction and modification (R-M) system, Sth368I, which confers resistance to phiST84, was found in Streptococcus thermophilus CNRZ368 but not in the very closely related strain A054. Partial sequencing of the integrative conjugative element ICESt1, carried by S. thermophilus CNRZ368 but not by A054,...

journal_title：Applied and environmental microbiology

authors： Burrus V,Bontemps C,Decaris B,Guédon G

更新日期：2001-04-01 00:00:00

abstract：:The presence of Bartonella spp. was investigated in domestic ungulates grazing in communal pastures from a mountain area in northern Spain, where 18.3% (17/93) of cattle were found to be positive by PCR combined with a reverse line blot (PCR/RLB), whereas sheep (n = 133) or horses (n = 91) were found not to be infecte...

journal_title：Applied and environmental microbiology

authors： Antequera-Gómez ML,Lozano-Almendral L,Barandika JF,González-Martín-Niño RM,Rodríguez-Moreno I,García-Pérez AL,Gil H

更新日期：2015-01-01 00:00:00