Abstract:
:Hydantoin racemase from Sinorhizobium meliloti was functionally expressed in Escherichia coli. The native form of the enzyme was a homotetramer with a molecular mass of 100 kDa. The optimum temperature and pH for the enzyme were 40 degrees C and 8.5, respectively. The enzyme showed a slight preference for hydantoins with short rather than long aliphatic side chains or those with aromatic rings. Substrates, which showed no detectable activity toward the enzyme, were found to exhibit competitive inhibition.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Martínez-Rodríguez S,Las Heras-Vázquez FJ,Mingorance-Cazorla L,Clemente-Jiménez JM,Rodríguez-Vico Fdoi
10.1128/aem.70.1.625-630.2004keywords:
subject
Has Abstractpub_date
2004-01-01 00:00:00pages
625-30issue
1eissn
0099-2240issn
1098-5336journal_volume
70pub_type
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