Abstract:
:The effects of Cd2+, Cu2+, Mg2+, and Zn2+ on the decomposition of citric acid by a Klebsiella sp. were studied by monitoring the degradation of [14C]citrate. The carbon concentration used was 10 micrograms of C liter-1, and the media were designed to provide at least 95% of the citrate complexed to the metal studied. After 72 h of incubation, 80% of the uncomplexed citric acid and 76% of the magnesium citrate had been decomposed. A marked inhibition was observed when Cd2+, Cu2+, or Zn2+ was bound to the organic anion; only 23% of the cadmium citrate, 14% of the zinc citrate, and 5% of the cuprous citrate had been decomposed. The effects were not the result of toxicity, since experiments run with [14C]glucose (nonchelating compound) instead of citrate resulted in similar decomposition rates regardless of the presence of the metal. To examine whether the binding of a metal to citrate enhanced its uptake by the Klebsiella sp., we studied the relative uptake of 65Zn in citrate- and in glucose-containing media. No such effect could be observed, with the uptake of Zn2+ being higher in the glucose-containing media. The study shows that metals may render low-molecular-weight organic acids, such as citric acid, resistant to bacterial degradation. This stresses the importance of metals in influencing microbial decomposition of organic compounds, not only as a result of toxicity.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Brynhildsen L,Rosswall Tdoi
10.1128/AEM.55.6.1375-1379.1989subject
Has Abstractpub_date
1989-06-01 00:00:00pages
1375-9issue
6eissn
0099-2240issn
1098-5336journal_volume
55pub_type
杂志文章abstract::Many microorganisms produce surface-active substances that enhance the availability of water-insoluble substrates. Although many of these biosurfactants have interesting potential applications, very little is known about their biosynthesis. The basidiomycetous fungus Ustilago maydis secretes large amounts of mannosyle...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.00506-06
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pub_type: 杂志文章
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更新日期:1995-12-01 00:00:00
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doi:10.1128/AEM.41.3.652-656.1981
更新日期:1981-03-01 00:00:00
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pub_type: 杂志文章
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pub_type: 杂志文章
doi:10.1128/AEM.59.3.763-771.1993
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2008-10-01 00:00:00
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pub_type: 杂志文章
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更新日期:2002-09-01 00:00:00
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pub_type: 杂志文章
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更新日期:2004-03-01 00:00:00