Significance of Extracellular Protease for Growth of a Heterotrophic Bacterium, Aeromonas salmonicida.

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:A single gene (xylB) encoding both beta-D-xylosidase (EC 3.2.1.37) and alpha-L-arabinofuranosidase (EC 3.2.1.55) activities was identified and sequenced from the ruminal bacterium Butyrivibrio fibrisolvens. The xylB gene consists of a 1.551-bp open reading frame (ORF) encoding 517 amino acids. A subclone containing a ...

journal_title：Applied and environmental microbiology

authors： Utt EA,Eddy CK,Keshav KF,Ingram LO

更新日期：1991-04-01 00:00:00

abstract：:Eubacterium lentum is a gram-positive, nonsporeforming, nonmotile, asaccharolytic anaerobe. In the present investigations, 3 E. lentum strains (group E) isolated from rat feces were compared with 30 E. lentum strains (groups A, B, C, and D) previously studied by Macdonald et al. (I. A. Macdonald, J. F. Jellet, D. E. M...

journal_title：Applied and environmental microbiology

authors： Eyssen H,Verhulst A

更新日期：1984-01-01 00:00:00

abstract：:Decisions as to the disposition of swine carcasses with lesions attributable to a mycobacterial infection are based upon a lesion criterion which is used as a diagnostic test by federal meat inspectors. Using this criterion, a federal meat inspector classified 58 of 100 pigs as "passed for cooking" and the other 42 pi...

journal_title：Applied and environmental microbiology

authors： Brown J,Neuman MA

更新日期：1979-04-01 00:00:00

abstract：:Denitrifying microbial communities and denitrification in salt marsh sediments may be affected by many factors, including environmental conditions, nutrient availability, and levels of pollutants. The objective of this study was to examine how microbial community composition and denitrification enzyme activities (DEA)...

journal_title：Applied and environmental microbiology

authors： Cao Y,Green PG,Holden PA

更新日期：2008-12-01 00:00:00

abstract：:The anti-norovirus (anti-NoV) effect of grape seed extract (GSE) was examined by plaque assay for murine norovirus 1 (MNV-1), cell-binding reverse transcription-PCR for human NoV GII.4, and saliva-binding enzyme-linked immunosorbent assay for human NoV GII.4 P particles, with or without the presence of interfering sub...

journal_title：Applied and environmental microbiology

authors： Li D,Baert L,Zhang D,Xia M,Zhong W,Van Coillie E,Jiang X,Uyttendaele M

更新日期：2012-11-01 00:00:00

abstract：:Mineralization of [U-(14)C]methyl t-butyl ether (MTBE) to (14)CO(2) without accumulation of t-butyl alcohol (TBA) was observed in surface-water sediment microcosms under denitrifying conditions. Methanogenic activity and limited transformation of MTBE to TBA were observed in the absence of denitrification. Results ind...

journal_title：Applied and environmental microbiology

authors： Bradley PM,Chapelle FH,Landmeyer JE

更新日期：2001-04-01 00:00:00

abstract：:The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of dete...

journal_title：Applied and environmental microbiology

authors： Raskin L,Capman WC,Kane MD,Rittmann BE,Stahl DA

更新日期：1996-01-01 00:00:00

abstract：:A separation procedure for the analysis of the enzyme components of the hyperthermophilic bacterium Thermotoga maritima involved in cellulose and xylan degradation was developed. Resolution of the enzymes was achieved by a combination of fast protein liquid chromatography anion exchange and hydrophobic interaction chr...

journal_title：Applied and environmental microbiology

authors： Bronnenmeier K,Kern A,Liebl W,Staudenbauer WL

更新日期：1995-04-01 00:00:00

abstract：:Sodium salicylate and ammonium sulfate were applied to leaf surfaces along with suspensions of the biological control agents Pseudomonas syringae Cit7(pNAH7), which catabolizes salicylate, and Cit7, which does not catabolize salicylate, to determine whether enhanced biological control of bacterial speck of tomato coul...

journal_title：Applied and environmental microbiology

authors： Ji P,Wilson M

更新日期：2003-02-01 00:00:00

abstract：:Submicronic particles released from fungal cultures have been suggested to be additional sources of personal exposure in mold-contaminated buildings. In vitro generation of these particles has been studied with particle counters, eventually supplemented by autofluorescence, that recognize fragments by size and discrim...

journal_title：Applied and environmental microbiology

authors： Afanou KA,Straumfors A,Skogstad A,Nilsen T,Synnes O,Skaar I,Hjeljord L,Tronsmo A,Green BJ,Eduard W

更新日期：2014-11-01 00:00:00

abstract：:Nontoxigenic strains of Clostridium botulinum types C and D are converted to toxigenic strains by infection with specific Tox+ bacteriophages. The nucleic acids were extracted from five converting phages, c-st, c-468, c-203, c-d6f, and d-1873, and one nonconverting phage, c-n71, and treated with nucleases. The nucleic...

journal_title：Applied and environmental microbiology

authors： Fujii N,Oguma K,Yokosawa N,Kimura K,Tsuzuki K

更新日期：1988-01-01 00:00:00

abstract：:Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

journal_title：Applied and environmental microbiology

authors： Dojka MA,Harris JK,Pace NR

更新日期：2000-04-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

journal_title：Applied and environmental microbiology

authors： Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

更新日期：2003-02-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:An epoxyalkane:coenzyme M (CoM) transferase (EaCoMT) enzyme was recently found to be active in the aerobic vinyl chloride (VC) and ethene assimilation pathways of Mycobacterium strain JS60. In the present study, EaCoMT activity and genes were investigated in 10 different mycobacteria isolated on VC or ethene from dive...

journal_title：Applied and environmental microbiology

authors： Coleman NV,Spain JC

更新日期：2003-10-01 00:00:00

abstract：:Vibrio parahaemolyticus is indigenous to coastal environments and a frequent cause of seafood-borne gastroenteritis in the United States, primarily due to raw-oyster consumption. Previous seasonal-cycle studies of V. parahaemolyticus have identified water temperature as the strongest environmental predictor. Salinity ...

journal_title：Applied and environmental microbiology

authors： Zimmerman AM,DePaola A,Bowers JC,Krantz JA,Nordstrom JL,Johnson CN,Grimes DJ

更新日期：2007-12-01 00:00:00

abstract：:Presently, the only effective treatment for celiac disease is a life-long gluten-free diet. In this work, we used a new mixture of selected sourdough lactobacilli and fungal proteases to eliminate the toxicity of wheat flour during long-time fermentation. Immunological (R5 antibody-based sandwich and competitive enzym...

journal_title：Applied and environmental microbiology

authors： Rizzello CG,De Angelis M,Di Cagno R,Camarca A,Silano M,Losito I,De Vincenzi M,De Bari MD,Palmisano F,Maurano F,Gianfrani C,Gobbetti M

更新日期：2007-07-01 00:00:00

abstract：:Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

journal_title：Applied and environmental microbiology

authors： Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

更新日期：2019-03-22 00:00:00

abstract：:Reductive dechlorination of the ortho moiety of polychlorinated biphenyls (PCBs) as well as of meta and para moieties is shown to occur in anaerobic enrichments of Baltimore Harbor sediments. These estuarine sediments ortho dechlorinated 2,3,5,6-chlorinated biphenyl (CB), 2,3,5-CB, and 2,3,6-CB in freshwater or estuar...

journal_title：Applied and environmental microbiology

authors： Berkaw M,Sowers KR,May HD

更新日期：1996-07-01 00:00:00

abstract：:Decomposition of natural populations of Lake Mendota phytoplankton dominated by blue-green algae (cyanobacteria) was monitored by using oxygen uptake and disappearance of chlorophyll, algal volume (fluorescence microscopy), particulate protein, particulate organic carbon, and photosynthetic ability (CO(2) up-take). In...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Brock TD

更新日期：1979-05-01 00:00:00

abstract：:Biochemical and proteomic tools have been utilized for investigating the mechanism of action of a new Stenotrophomonas maltophilia strain (SeITE02), a gammaproteobacterium capable of resistance to high concentrations of selenite [SeO(3)(2-), Se(IV)], reducing it to nontoxic elemental selenium under aerobic conditions;...

journal_title：Applied and environmental microbiology

authors： Antonioli P,Lampis S,Chesini I,Vallini G,Rinalducci S,Zolla L,Righetti PG

更新日期：2007-11-01 00:00:00

abstract：:The use of the food-grade bacterium Lactococcus lactis as a DNA delivery vehicle at the mucosal level is an attractive DNA vaccination strategy. Previous experiments showed that recombinant L. lactis expressing the Listeria monocytogenes inlA gene can deliver a functional gene into mammalian cells. Here, we explored t...

journal_title：Applied and environmental microbiology

authors： Guimarães VD,Innocentin S,Lefèvre F,Azevedo V,Wal JM,Langella P,Chatel JM

更新日期：2006-11-01 00:00:00

abstract：:We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of ap...

journal_title：Applied and environmental microbiology

authors： Stepkowski T,Moulin L,Krzyzańska A,McInnes A,Law IJ,Howieson J

更新日期：2005-11-01 00:00:00

abstract：:A stable bacterial community absorbed and transformed the herbicide metolachlor [2-chloro-N-(2-ethyl- 6-methylphenyl)-N-(2-methoxy-1-methylethyl)-acetamide] from a liquid medium. About 80% of the added ring-[U-C]metolachlor (50 mug/ml) disappeared from the medium and accumulated inside the cells. The ratio of cellular...

journal_title：Applied and environmental microbiology

authors： Liu SY,Zheng Z,Zhang R,Bollag JM

更新日期：1989-03-01 00:00:00

abstract：:The relationship between the production of reduced oxygen species, hydrogen peroxide (H(2)O(2)), superoxide (O(2)), and hydroxyl radical (.OH), and the oxidation of synthetic lignin to CO(2) was studied in whole cultures of the white-rot fungus Phanerochaete chrysosporium Burds. The kinetics of the synthesis of H(2)O(...

journal_title：Applied and environmental microbiology

authors： Faison BD,Kirk TK

更新日期：1983-11-01 00:00:00

abstract：:High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...

journal_title：Applied and environmental microbiology

authors： Beffa T,Blanc M,Lyon PF,Vogt G,Marchiani M,Fischer JL,Aragno M

更新日期：1996-05-01 00:00:00

abstract：:The psychrotolerant acidophile Acidithiobacillus ferrivorans has been identified from cold environments and has been shown to use ferrous iron and inorganic sulfur compounds as its energy sources. A bioinformatic evaluation presented in this study suggested that Acidithiobacillus ferrivorans utilized a ferrous iron ox...

journal_title：Applied and environmental microbiology

authors： Liljeqvist M,Rzhepishevska OI,Dopson M

更新日期：2013-02-01 00:00:00

abstract：:Laboratory-scale soil microcosms containing different soils were permeated with CH(inf4) for up to 6 months to investigate their capacity to develop a methanotrophic community. Methane emissions were monitored continuously until steady states were established. The porous, coarse sand soil developed the greatest methan...

journal_title：Applied and environmental microbiology

authors： Kightley D,Nedwell DB,Cooper M

更新日期：1995-02-01 00:00:00