Abstract:
:Laboratory-scale soil microcosms containing different soils were permeated with CH(inf4) for up to 6 months to investigate their capacity to develop a methanotrophic community. Methane emissions were monitored continuously until steady states were established. The porous, coarse sand soil developed the greatest methanotrophic capacity (10.4 mol of CH(inf4) (middot) m(sup-2) (middot) day(sup-1)), the greatest yet reported in the literature. Vertical profiles of O(inf2), CH(inf4), and methanotrophic potential in the soils were determined at steady state. Methane oxidation potentials were greatest where the vertical profiles of O(inf2) and CH(inf4) overlapped. A significant increase in the organic matter content of the soil, presumably derived from methanotroph biomass, occurred where CH(inf4) oxidation was greatest. Methane oxidation kinetics showed that a soil community with a low methanotrophic capacity (V(infmax) of 258 nmol (middot) g of soil(sup-1) (middot) h(sup-1)) but relatively high affinity (k(infapp) of 1.6 (mu)M) remained in N(inf2)-purged control microcosms, even after 6 months without CH(inf4). We attribute this to a facultative, possibly mixotrophic, methanotrophic microbial community. When purged with CH(inf4), a different methanotrophic community developed which had a lower affinity (k(infapp) of 31.7 (mu)M) for CH(inf4) but a greater capacity (V(infmax) of 998 nmol (middot) g of soil(sup-1) (middot) h(sup-1)) for CH(inf4) oxidation, reflecting the enrichment of an active high-capacity methanotrophic community. Compared with the unamended control soil, amendment of the coarse sand with sewage sludge enhanced CH(inf4) oxidation capacity by 26%; K(inf2)HPO(inf4) amendment had no significant effect, while amendment with NH(inf4)NO(inf3) reduced the CH(inf4) oxidation capacity by 64%. In vitro experiments suggested that NH(inf4)NO(inf3) additions (10 and 71 (mu)mol (middot) g of soil(sup-1)) inhibited CH(inf4) oxidation by a nonspecific ionic effect rather than by specific inhibition by NH(inf4)(sup+).
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Kightley D,Nedwell DB,Cooper Mdoi
10.1128/AEM.61.2.592-601.1995keywords:
subject
Has Abstractpub_date
1995-02-01 00:00:00pages
592-601issue
2eissn
0099-2240issn
1098-5336journal_volume
61pub_type
杂志文章abstract::Methane production and archaeal community composition were studied in samples from an acidic peat bog incubated at different temperatures and pH values. H(2)-dependent methanogenesis increased strongly at the lowest pH, 3.8, and Methanobacteriaceae became important except for Methanomicrobiaceae and Methanosarcinaceae...
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.67.5.2051-2055.2001
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abstract::Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially puri...
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doi:10.1128/AEM.52.5.1026-1030.1986
更新日期:1986-11-01 00:00:00
abstract::Micro-Raman spectroscopy is a fast and sensitive tool for the detection, classification, and identification of biological organisms. The vibrational spectrum inherently serves as a fingerprint of the biochemical composition of each bacterium and thus makes identification at the species level, or even the subspecies le...
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更新日期:1985-10-01 00:00:00
abstract::Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypotheti...
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更新日期:1981-07-01 00:00:00
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doi:10.1128/AEM.54.3.753-759.1988
更新日期:1988-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/aem.69.12.6987-6993.2003
更新日期:2003-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.3.1190-1197.2001
更新日期:2001-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.11.3346-3353.1990
更新日期:1990-11-01 00:00:00
abstract::[This corrects the article on p. 507 in vol. 30.]. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:
更新日期:1983-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2020-03-02 00:00:00
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doi:10.1128/AEM.72.2.1459-1466.2006
更新日期:2006-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.67.5.2388-2392.2001
更新日期:2001-05-01 00:00:00
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更新日期:1997-02-01 00:00:00
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更新日期:1986-12-01 00:00:00
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doi:10.1128/AEM.55.11.2856-2860.1989
更新日期:1989-11-01 00:00:00
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更新日期:2009-02-01 00:00:00
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更新日期:2000-01-01 00:00:00
abstract::The alphaproteobacterium Hyphomonas neptunium proliferates by a unique budding mechanism in which daughter cells emerge from the end of a stalk-like extension emanating from the mother cell body. Studies of this species so far have been hampered by the lack of a genetic system and of molecular tools allowing the regul...
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更新日期:2015-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1985-07-01 00:00:00