Degradation of the Ferric Chelate of EDTA by a Pure Culture of an Agrobacterium sp.

Abstract:

:A pure culture of an Agrobacterium sp. (deposited as ATCC 55002) that mineralizes the ferric chelate of EDTA (ferric-EDTA) was isolated by selective enrichment from a treatment facility receiving industrial waste containing ferric-EDTA. The isolate grew on ferric-EDTA as the sole carbon source at concentrations exceeding 100 mM. As the degradation proceeded, carbon dioxide, ammonia, and an unidentified metabolite(s) were produced; the pH increased, and iron was precipitated from solution. The maximum rate of degradation observed with sodium ferric-EDTA as the substrate was 24 mM/day. At a substrate concentration of 35 mM, 90% of the substrate was degraded in 3 days and 70% of the associated chemical oxygen demand was removed from solution. Less than 15% of the carbon initially present was incorporated into the cell mass. Significant growth of this strain was not observed with uncomplexed EDTA as the sole carbon source at comparable concentrations; however, the ferric chelate of propylenediaminetetraacetic acid (ferric-PDTA) did support growth.

journal_name

Appl Environ Microbiol

authors

Lauff JJ,Steele DB,Coogan LA,Breitfeller JM

doi

10.1128/AEM.56.11.3346-3353.1990

keywords:

subject

Has Abstract

pub_date

1990-11-01 00:00:00

pages

3346-53

issue

11

eissn

0099-2240

issn

1098-5336

journal_volume

56

pub_type

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