Ribotyping to differentiate Fusobacterium necrophorum subsp. necrophorum and F. necrophorum subsp. funduliforme isolated from bovine ruminal contents and liver abscesses.

Abstract:

:Differences in biological activities (hemagglutination, hemolytic, leukotoxic, and virulence) and ribotypes between the two subspecies of Fusobacterium necrophorum of bovine ruminal and liver abscess origins were investigated. Hemagglutination activity was present in all hepatic, but only some ruminal, strains of Fusobacterium necrophorum subsp. necrophorum. Ruminal F. necrophorum subsp. necrophorum had low leukotoxin titers yet was virulent in mice. Fusobacterium necrophorum subsp. funduliforme of hepatic or ruminal origin had no hemagglutination activity, had low hemolytic and leukotoxic activities, and was less virulent to mice. For ribotyping, chromosomal DNAs of 10 F. necrophorum subsp. necrophorum and 11 F. necrophorum subsp. funduliforme isolates were digested with restriction endonucleases (EcoRI, EcoRV, SalI, PstI, and HaeIII) and examined by restriction fragment length polymorphisms after hybridizing with a digoxigenin-labeled cDNA probe transcribed from a mixture of 16 and 23S rRNAs from Escherichia coli. The most discriminating restriction endonuclease enzyme for ribotyping was EcoRI. The presence or absence of two distinct bands of 2.6 and 4.3 kb differentiated the two subspecies. Regardless of the origin, only F. necrophorum subsp. necrophorum, a virulent subspecies, had a ca. 2.6-kb band, whereas F. necrophorum subsp. funduliforme, a less virulent subspecies, had a ca. 4.3-kb band. Ribotyping appears to be a useful technique to genetically differentiate the two subspecies of F. necrophorum.

journal_name

Appl Environ Microbiol

authors

Okwumabua O,Tan Z,Staats J,Oberst RD,Chengappa MM,Nagaraja TG

doi

10.1128/AEM.62.2.469-472.1996

subject

Has Abstract

pub_date

1996-02-01 00:00:00

pages

469-72

issue

2

eissn

0099-2240

issn

1098-5336

journal_volume

62

pub_type

杂志文章
  • Roles of DacB and spm proteins in clostridium perfringens spore resistance to moist heat, chemicals, and UV radiation.

    abstract::Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that alpha/beta-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00169-08

    authors: Paredes-Sabja D,Sarker N,Setlow B,Setlow P,Sarker MR

    更新日期:2008-06-01 00:00:00

  • The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway.

    abstract::Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two pa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01401-08

    authors: Kolkman MA,van der Ploeg R,Bertels M,van Dijk M,van der Laan J,van Dijl JM,Ferrari E

    更新日期:2008-12-01 00:00:00

  • Response of a soil bacterial community to grassland succession as monitored by 16S rRNA levels of the predominant ribotypes.

    abstract::The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.3998-4003.2000

    authors: Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

    更新日期:2000-09-01 00:00:00

  • Protein method for investigating mercuric reductase gene expression in aquatic environments.

    abstract::A colorimetric assay for NADPH-dependent, mercuric ion-specific oxidoreductase activity was developed to facilitate the investigation of mercuric reductase gene expression in polluted aquatic ecosystems. Protein molecules extracted directly from unseeded freshwater and samples seeded with Pseudomonas aeruginosa PU21 (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.2.695-702.1998

    authors: Ogunseitan OA

    更新日期:1998-02-01 00:00:00

  • Links between plant and rhizoplane bacterial communities in grassland soils, characterized using molecular techniques.

    abstract::Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most clo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.6784-6792.2005

    authors: Nunan N,Daniell TJ,Singh BK,Papert A,McNicol JW,Prosser JI

    更新日期:2005-11-01 00:00:00

  • Redox chemistry in laccase-catalyzed oxidation of N-hydroxy compounds.

    abstract::1-Hydroxybenzotriazole, violuric acid, and N-hydroxyacetanilide are three N-OH compounds capable of mediating a range of laccase-catalyzed biotransformations, such as paper pulp delignification and degradation of polycyclic hydrocarbons. The mechanism of their enzymatic oxidation was studied with seven fungal laccases...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.5.2052-2056.2000

    authors: Xu F,Kulys JJ,Duke K,Li K,Krikstopaitis K,Deussen HJ,Abbate E,Galinyte V,Schneider P

    更新日期:2000-05-01 00:00:00

  • Cytopathology of pathogenic and nonpathogenic Naegleria species for cultured rat neuroblastoma cells.

    abstract::The cytopathology for rat neuroblastoma cells (B-103) and the pathogenicity for B6C3F1 mice of four species of Naegleria have been compared. Both live amoebae and cell-free extracts of N. australiensis, N. fowleri, N. gruberi, and N. lovaniensis added to 51Cr-labeled B-103 cells caused release of radiolabel. All four ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.51.5.1133-1137.1986

    authors: Marciano-Cabral FM,Fulford DE

    更新日期:1986-05-01 00:00:00

  • Secreted factors from Bifidobacterium animalis subsp. lactis inhibit NF-κB-mediated interleukin-8 gene expression in Caco-2 cells.

    abstract::The objective of the present study was to evaluate the anti-inflammatory effects of Bifidobacterium animalis subsp. lactis strain BB12 in stimulated Caco-2 cells and to characterize the factors responsible for these anti-inflammatory effects. Characterization and purification studies indicate that BB12's anti-inflamma...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06145-11

    authors: Wang Z,Wang J,Cheng Y,Liu X,Huang Y

    更新日期:2011-11-01 00:00:00

  • Characterization of the biosynthetic operon for the antibacterial peptide herbicolin in Pantoea vagans biocontrol strain C9-1 and incidence in Pantoea species.

    abstract::Pantoea vagans C9-1 is a biocontrol strain that produces at least two antibiotics inhibiting the growth of Erwinia amylovora, the causal agent of fire blight disease of pear and apple. One antibiotic, herbicolin I, was purified from culture filtrates of P. vagans C9-1 and determined to be 2-amino-3-(oxirane-2,3-dicarb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07351-11

    authors: Kamber T,Lansdell TA,Stockwell VO,Ishimaru CA,Smits TH,Duffy B

    更新日期:2012-06-01 00:00:00

  • Rapid and simple cryopreservation of anaerobic ammonium-oxidizing bacteria.

    abstract::A quick and simple protocol for long-term cryopreservation of anaerobic ammonium-oxidizing bacteria (anammox bacteria) was developed. After 29 weeks of preservation at -80°C, activity recovery for all tested cultures under at least one of the applied sets of preservation conditions was observed. Growth recovery was al...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07501-11

    authors: Heylen K,Ettwig K,Hu Z,Jetten M,Kartal B

    更新日期:2012-04-01 00:00:00

  • Purification and Characterization of Three Chitosanase Activities from Bacillus megaterium P1.

    abstract::Bacillus megaterium P1, a bacterial strain capable of hydrolyzing chitosan, was isolated from soil samples. Chitosan-degrading activity was induced by chitosan but not by its constituent d-glucosamine. Extracellular secretion of chitosanase reached levels corresponding to 1 U/ml under optimal conditions. Three chitosa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.844-848.1990

    authors: Pelletier A,Sygusch J

    更新日期:1990-04-01 00:00:00

  • Polyamine transporters and polyamines increase furfural tolerance during xylose fermentation with ethanologenic Escherichia coli strain LY180.

    abstract::Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). This represents a new approach to increase furfural tolerance and may be usef...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01913-14

    authors: Geddes RD,Wang X,Yomano LP,Miller EN,Zheng H,Shanmugam KT,Ingram LO

    更新日期:2014-10-01 00:00:00

  • Role of phaD in accumulation of medium-chain-length Poly(3-hydroxyalkanoates) in Pseudomonas oleovorans.

    abstract::Pseudomonas oleovorans is capable of producing poly(3-hydroxyalkanoates) (PHAs) as intracellular storage material. To analyze the possible involvement of phaD in medium-chain-length (MCL) PHA biosynthesis, we generated a phaD knockout mutant by homologous recombination. Upon disruption of the phaD gene, MCL PHA polyme...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.9.3705-3710.2000

    authors: Klinke S,de Roo G,Witholt B,Kessler B

    更新日期:2000-09-01 00:00:00

  • Modifying the Lantibiotic Mutacin 1140 for Increased Yield, Activity, and Stability.

    abstract::Mutacin 1140 belongs to the epidermin family of type AI lantibiotics. This family has a broad spectrum of activity against Gram-positive bacteria. The binding of mutacin 1140 to lipid II leads to the inhibition of cell wall synthesis. Pharmacokinetic experiments with type AI lantibiotics are generally discouraging for...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00830-18

    authors: Geng M,Smith L

    更新日期:2018-07-17 00:00:00

  • Purification and Characterization of a Substrate-Size-Recognizing Metalloendopeptidase from Streptococcus cremoris H61.

    abstract::During the ripening of Gouda-type cheese, two kinds of endopeptidases were found to participate in the degradation of alphas1-CN(f1-23), a specific product from alphas1-casein hydrolyzed by chymosin. One of the endopeptidases, lactic acid bacteria endopeptidase (LEP-II), which can recognize the size of its substrates,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2296-2302.1987

    authors: Yan TR,Azuma N,Kaminogawa S,Yamauchi K

    更新日期:1987-10-01 00:00:00

  • Culture-independent analysis of fecal enterobacteria in environmental samples by single-cell mRNA profiling.

    abstract::A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4432-4439.2004

    authors: Chen H,Ponniah G,Salonen N,Blum P

    更新日期:2004-08-01 00:00:00

  • Electron Spin Resonance Analysis of the Effect of Butanol on the Membrane Fluidity of Intact Cells of Clostridium acetobutylicum.

    abstract::Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.10.2729-2731.1989

    authors: Baer SH,Bryant DL,Blaschek HP

    更新日期:1989-10-01 00:00:00

  • Identification of capsule-forming Bacillus anthracis spores with the PCR and a novel dual-probe hybridization format.

    abstract::Anthrax is a fatal infection of humans and livestock that is caused by the gram-positive bacterium Bacillus anthracis. The virulent strains of B. anthracis are encapsulated and toxigenic. In this paper we describe the development of a PCR technique for identifying spores of B. anthracis. Two 20-mer oligonucleotide pri...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.5.1622-1625.1994

    authors: Reif TC,Johns M,Pillai SD,Carl M

    更新日期:1994-05-01 00:00:00

  • Use of murine norovirus as a surrogate to evaluate resistance of human norovirus to disinfectants.

    abstract::Murine norovirus (MNV) was used as a surrogate to study resistance of human norovirus to disinfectants used in hospitals. MNV was sensitive to alcohol, alcohol hand rubs, bleach, and povidone iodine-based disinfectant. Real-time reverse transcription-PCR results indicated that the presence of viral RNA did not correla...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02148-07

    authors: Belliot G,Lavaux A,Souihel D,Agnello D,Pothier P

    更新日期:2008-05-01 00:00:00

  • Development of a Defined Medium for Growth of Cercospora rosicola Passerini.

    abstract::A simple synthetic liquid medium containing a single amino acid, glucose, salts, trace metals, and thiamine was developed for cultivation of Cercospora rosicola Passerini. Thiamine was shown to be important to growth. Culture of C. rosicola Passerini in a chemically defined medium makes possible studies of (+)-abscisi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.1.334-336.1981

    authors: Norman SM,Maier VP,Echols LC

    更新日期:1981-01-01 00:00:00

  • Rapid genomic fingerprinting of Lactococcus lactis strains by arbitrarily primed polymerase chain reaction with 32P and fluorescent labels.

    abstract::Arbitrarily primed polymerase chain reaction, with incorporation of either radioactive or fluorescent labels, was used as a rapid and sensitive method for obtaining genomic fingerprints of strains of Lactococcus lactis. Closely related strains produced almost identical fingerprints. Fingerprints of other strains showe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.5.1772-1775.1992

    authors: Cancilla MR,Powell IB,Hillier AJ,Davidson BE

    更新日期:1992-05-01 00:00:00

  • Fibronectin enhances transfection of Staphylococcus aureus.

    abstract::The factor in normal sera primarily responsible for the enhancement of transfection (and transformation) of Staphylococcus aureus was identified as fibronectin. Serum samples which were depleted of fibronectin by affinity chromatography showed a marked decrease in enhancing activity. Fibronectin isolated from sera of ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.5.1315-1318.1985

    authors: Thompson NE,Bergdoll MS,Pattee PA

    更新日期:1985-11-01 00:00:00

  • Temperature-driven adaptation of the bacterial community in peat measured by using thymidine and leucine incorporation.

    abstract::The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.3.1116-1122.2001

    authors: Ranneklev SB,Bååth E

    更新日期:2001-03-01 00:00:00

  • Ultrastructural examination of Aeromonas cultured in the presence of organic lead.

    abstract::Aeromonas sp. will methylate trimethyl lead acetate (Me3PbOAc) to volatile tetramethyl lead (Me4Pb). Examination of cultures grown in the presence of Me3PbOAc revealed no major irregularities between cells of the treated and untreated cultures. Some cells, however, showed evidence that intracytoplasmic materials had b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.5.723-725.1976

    authors: Silverberg BA,Wong PT,Chau YK

    更新日期:1976-11-01 00:00:00

  • Isolation of tellurite- and selenite-resistant bacteria from hydrothermal vents of the Juan de Fuca Ridge in the Pacific Ocean.

    abstract::Deep-ocean hydrothermal-vent environments are rich in heavy metals and metalloids and present excellent sites for the isolation of metal-resistant microorganisms. Both metalloid-oxide-resistant and metalloid-oxide-reducing bacteria were found. Tellurite- and selenite-reducing strains were isolated in high numbers from...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.9.4613-4622.2002

    authors: Rathgeber C,Yurkova N,Stackebrandt E,Beatty JT,Yurkov V

    更新日期:2002-09-01 00:00:00

  • Involvement of acyl coenzyme A oxidase isozymes in biotransformation of methyl ricinoleate into gamma-decalactone by Yarrowia lipolytica.

    abstract::We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lacto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.3.1233-1236.2000

    authors: Waché Y,Laroche C,Bergmark K,Møller-Andersen C,Aguedo M,Le Dall MT,Wang H,Nicaud JM,Belin JM

    更新日期:2000-03-01 00:00:00

  • Population Genetics and Characterization of Campylobacter jejuni Isolates from Western Jackdaws and Game Birds in Finland.

    abstract::Poultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n = 91, 43%), mallard ducks (n = 82, 76%), and phea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02365-18

    authors: Kovanen S,Rossi M,Pohja-Mykrä M,Nieminen T,Raunio-Saarnisto M,Sauvala M,Fredriksson-Ahomaa M,Hänninen ML,Kivistö R

    更新日期:2019-02-06 00:00:00

  • Postgenomic analysis of streptococcus thermophilus cocultivated in milk with Lactobacillus delbrueckii subsp. bulgaricus: involvement of nitrogen, purine, and iron metabolism.

    abstract::Streptococcus thermophilus is one of the most widely used lactic acid bacteria in the dairy industry, in particular in yoghurt manufacture, where it is associated with Lactobacillus delbrueckii subsp. bulgaricus. This bacterial association, known as a proto-cooperation, is poorly documented at the molecular and regula...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01984-08

    authors: Herve-Jimenez L,Guillouard I,Guedon E,Boudebbouze S,Hols P,Monnet V,Maguin E,Rul F

    更新日期:2009-04-01 00:00:00

  • Urease-encoding genes in ammonia-oxidizing bacteria.

    abstract::Many but not all ammonia-oxidizing bacteria (AOB) produce urease (urea amidohydrolase, EC 3.5.1.5) and are capable of using urea for chemolithotrophic growth. We sequenced the urease operons from two AOB, the beta-proteobacterium Nitrosospira sp. strain NpAV and the gamma-proteobacterium Nitrosococcus oceani. In both ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.4.2342-2348.2004

    authors: Koper TE,El-Sheikh AF,Norton JM,Klotz MG

    更新日期:2004-04-01 00:00:00

  • Integrative conjugative elements are widespread in field isolates of Mycoplasma species pathogenic for ruminants.

    abstract::Comparative genomics have revealed massive horizontal gene transfer (HGT) between Mycoplasma species sharing common ruminant hosts. Further results pointed toward an integrative conjugative element (ICE) as an important contributor of HGT in the small-ruminant-pathogen Mycoplasma agalactiae. To estimate the prevalence...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03723-14

    authors: Tardy F,Mick V,Dordet-Frisoni E,Marenda MS,Sirand-Pugnet P,Blanchard A,Citti C

    更新日期:2015-03-01 00:00:00