Liquid serial dilution is inferior to solid media for isolation of cultures representative of the phylum-level diversity of soil bacteria.

abstract：:A postgastric, fermentative breakdown of structural plant tissue was demonstrated for green turtles. About 90% cellulose was hydrolyzed. Bacterial and protozoan numbers compared with those of the rumen. ...

journal_title：Applied and environmental microbiology

authors： Fenchel TM,McRoy CP,Ogden JC,Parker P,Rainey WE

更新日期：1979-02-01 00:00:00

abstract：:Iron(III)-reducing bacteria have been demonstrated to rapidly catalyze the reduction and immobilization of uranium(VI) from contaminated subsurface sediments. Thus, these organisms may aid in the development of bioremediation strategies for uranium contamination, which is prevalent in acidic subsurface sediments at U....

journal_title：Applied and environmental microbiology

authors： Petrie L,North NN,Dollhopf SL,Balkwill DL,Kostka JE

更新日期：2003-12-01 00:00:00

abstract：:Aromatic hydroxylations are important bacterial metabolic processes but are difficult to perform using traditional chemical synthesis, so to use a biological catalyst to convert the priority pollutant benzene into industrially relevant intermediates, benzene oxidation was investigated. It was discovered that toluene 4...

journal_title：Applied and environmental microbiology

authors： Tao Y,Fishman A,Bentley WE,Wood TK

更新日期：2004-07-01 00:00:00

abstract：:Two bacteriolytic enzymes were produced when Hartmanella glebae was grown in the presence of both Enterobacter aerogenes and Alcaligenes faecalis. The identification of enzyme I as N-acetylmuramidase was reported earlier. Enzyme II was purified by gel filtration on a Bio-Gel A column. A recovery of 68.76% with 72.3-fo...

journal_title：Applied and environmental microbiology

authors： Hemelt DM,Mares B,Upadhyay JM

更新日期：1979-09-01 00:00:00

abstract：:Escherichia coli, commonly used in chemotaxis studies, is attracted mostly by amino acids, sugars, and peptides. We envisioned modifying the chemotaxis specificity of E. coli by expressing heterologous chemoreceptors from Pseudomonas putida enabling attraction either to toluene or benzoate. The mcpT gene encoding the ...

journal_title：Applied and environmental microbiology

authors： Roggo C,Clerc EE,Hadadi N,Carraro N,Stocker R,van der Meer JR

更新日期：2018-08-31 00:00:00

abstract：:The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry ...

journal_title：Applied and environmental microbiology

authors： Park JH,Szponar B,Larsson L,Gold DR,Milton DK

更新日期：2004-01-01 00:00:00

abstract：:The ability of Erwinia chrysanthemi to cope with environments of elevated osmolality is due in part to the transport and accumulation of osmoprotectants. In this study we have identified a high-affinity glycine betaine and choline transport system in E. chrysanthemi. By using a pool of Tn5-B21 ousA mutants, we isolate...

journal_title：Applied and environmental microbiology

authors： Choquet G,Jehan N,Pissavin C,Blanco C,Jebbar M

更新日期：2005-07-01 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu lato was assessed in a focus of Lyme borreliosis in southern Britain dominated by game birds. Ticks, rodents, and pheasants were analyzed for spirochete infections by PCR-targeting the 23S-5S rRNA genes, followed by genotyping by the reverse line blot method. In ques...

journal_title：Applied and environmental microbiology

authors： Kurtenbach K,Peacey M,Rijpkema SG,Hoodless AN,Nuttall PA,Randolph SE

更新日期：1998-04-01 00:00:00

abstract：:Some species of ruminal bacteria are known to produce antimicrobial peptides, but the screening procedures have mostly been based on in vitro assays using standardized methods. Recent sequencing efforts have made available the genome sequences of hundreds of ruminal microorganisms. In this work, we performed genome mi...

journal_title：Applied and environmental microbiology

authors： Azevedo AC,Bento CB,Ruiz JC,Queiroz MV,Mantovani HC

更新日期：2015-10-01 00:00:00

abstract：:A rich history of investigation documents various Drosophila-yeast mutualisms, suggesting that Drosophila suzukii similarly has an association with a specific yeast species or community. To discover candidate yeast species, yeasts were isolated from larval frass, adult midguts, and fruit hosts of D. suzukii. Terminal ...

journal_title：Applied and environmental microbiology

authors： Hamby KA,Hernández A,Boundy-Mills K,Zalom FG

更新日期：2012-07-01 00:00:00

abstract：:The steady-state effect of 2,5,2',5'-tetrachlorobiphenyl (TCBP) on the green alga Selenastrum capricornutum was investigated in a P-limited two-stage chemostat system. The partition coefficient of this polychlorinated biphenyl congener was 5.9 x 10 in steady-state cultures. At a cellular TCBP concentration of 12.2 x 1...

journal_title：Applied and environmental microbiology

authors： Rhee GY,Shane L,Denucci A

更新日期：1988-06-01 00:00:00

abstract：:Helicobacter pylori is considered one of the major risk factors underlying the development of gastritis and gastric and duodenal ulcers. Moreover, 50% of the population carries this bacterium, and consequently, when it is detected, eradication of H. pylori is strongly recommended. Regarding the use of probiotics as fu...

journal_title：Applied and environmental microbiology

authors： Chenoll E,Casinos B,Bataller E,Astals P,Echevarría J,Iglesias JR,Balbarie P,Ramón D,Genovés S

更新日期：2011-02-01 00:00:00

abstract：:Five different bacterial extracellular polymer extraction methods and a combination of two of these methods were compared on cultures of activated sludge, synthetic activated sludge, and Klebsiella aerogenes. High-speed centrifugation was the most effective extraction method for the K. aerogenes culture, based on the ...

journal_title：Applied and environmental microbiology

authors： Brown MJ,Lester JN

更新日期：1980-08-01 00:00:00

abstract：:Pervasive environmental stressors on coral reefs are attributed with shifting the competitive balance in favor of alternative dominants, such as macroalgae. Previous studies have demonstrated that macroalgae compete with corals via a number of mechanisms, including the production of potent primary and secondary metabo...

journal_title：Applied and environmental microbiology

authors： Morrow KM,Bromhall K,Motti CA,Munn CB,Bourne DG

更新日期：2016-12-15 00:00:00

abstract：:The effects of limiting concentrations of ammonium on the metabolic activity of Nitrosomonas europaea, an obligate ammonia-oxidizing soil bacterium, were investigated. Cells were harvested during late logarithmic growth and were incubated for 24 h in growth medium containing 0, 15, or 50 mM ammonium. The changes in ni...

journal_title：Applied and environmental microbiology

authors： Stein LY,Arp DJ

更新日期：1998-04-01 00:00:00

abstract：:This study examined the inactivation of human norovirus (HuNoV) GI.1 and GII.4 by chlorine under conditions mimicking sewage treatment. Using a porcine gastric mucin-magnetic bead (PGM-MB) assay, no statistically significant loss in HuNoV binding (inactivation) was observed for secondary effluent treatments of ≤25 ppm...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Fay JP,Calci K,Pouillot R,Woods J,Chen H,Niemira BA,Van Doren JM

更新日期：2017-11-16 00:00:00

abstract：:The microaerophilic bacterium Campylobacter jejuni is the most common cause of bacterial food-borne infections in the developed world. Tolerance to environmental stress relies on proteases and chaperones in the cell envelope, such as HtrA and SurA. HtrA displays both chaperone and protease activities, but little is kn...

journal_title：Applied and environmental microbiology

authors： Baek KT,Vegge CS,Skórko-Glonek J,Brøndsted L

更新日期：2011-01-01 00:00:00

abstract：:Biogenic amines may reach concentrations of public health concern in some cheeses. To minimize biogenic amine buildup in raw milk cheese, high-pressure treatments of 400 or 600 MPa for 5 min were applied on days 21 and 35 of ripening. On day 60, counts of lactic acid bacteria, enterococci, and lactobacilli were 1 to 2...

journal_title：Applied and environmental microbiology

authors： Calzada J,del Olmo A,Picón A,Gaya P,Nuñez M

更新日期：2013-02-01 00:00:00

abstract：:A rare strain of Clostridium botulinum subtype Ab was isolated from a canned macrobiotic food suspected of being linked to a fatal case of food-borne botulism. The strain was recovered and identified by conventional methods modified by the inclusion of a PCR assay (G. Franciosa, J.L. Ferreira, and C.L. Hatheway, J. Cl...

journal_title：Applied and environmental microbiology

authors： Franciosa G,Fenicia L,Pourshaban M,Aureli P

更新日期：1997-03-01 00:00:00

abstract：:Genome-wide analysis has revealed abundant FabG (beta-ketoacyl-ACP reductase) paralogs, with uncharacterized biological functions, in several halophilic archaea. In this study, we identified for the first time that the fabG1 gene, but not the other five fabG paralogs, encodes the polyhydroxyalkanoate (PHA)-specific ac...

journal_title：Applied and environmental microbiology

authors： Han J,Lu Q,Zhou L,Liu H,Xiang H

更新日期：2009-10-01 00:00:00

abstract：:Intermediates of production of two batches of traditional mozzarella cheese were analyzed by culture-independent pyrosequencing. The quantitative distribution of taxa within the samples suggested that thermophilic lactic acid bacteria from the natural starter were mainly responsible for the fermentation, while microor...

journal_title：Applied and environmental microbiology

authors： Ercolini D,De Filippis F,La Storia A,Iacono M

更新日期：2012-11-01 00:00:00

abstract：:Many microorganisms produce surface-active substances that enhance the availability of water-insoluble substrates. Although many of these biosurfactants have interesting potential applications, very little is known about their biosynthesis. The basidiomycetous fungus Ustilago maydis secretes large amounts of mannosyle...

journal_title：Applied and environmental microbiology

authors： Hewald S,Linne U,Scherer M,Marahiel MA,Kämper J,Bölker M

更新日期：2006-08-01 00:00:00

abstract：:The stability of spheroplasts from the osmotrophic yeast Saccharomyces rouxii was studied in buffered solutions of mannitol and glucose. The plasma membranes from cells grown in high glucose concentrations were more stable to osmotic lysis than were membranes from cells grown in lower glucose concentrations. Mannitol ...

journal_title：Applied and environmental microbiology

authors： Moran JW,Witter LD

更新日期：1980-04-01 00:00:00

abstract：:A mathematical model was developed to estimate the number of logarithmic cycles (LDec) of lipopolysaccharide concentration destroyed by a dry-heat sterilization process. The LDec values calculated from the mathematical model agreed well with those obtained from the destruction of lipopolysaccharide by a dry-heat treat...

journal_title：Applied and environmental microbiology

authors： Tsuji K,Lewis AR

更新日期：1978-11-01 00:00:00

abstract：:The kinetics of concentration-dependent toluene metabolism were examined by evaluating each term in the second-order rate equation. Marine and freshwater pseudomonads were used. Uptake for Pseudomonas sp. strain T2 was characterized by a completely saturatable system with small transport constant (K(t) = 44 mug/liter)...

journal_title：Applied and environmental microbiology

authors： Robertson BR,Button DK

更新日期：1987-09-01 00:00:00

abstract：:Conditioned medium was obtained from suspension cultures of soybean (Glycine max L. Merrit) cells after incubating them for 4 to 8 days with rhizobia which were separated from the soybean cells by two dialysis bags, one within another. This conditioned medium from the plant cell side (PCM) of the two membranes was use...

journal_title：Applied and environmental microbiology

authors： Bednarski MA,Reporter M

更新日期：1978-07-01 00:00:00

abstract：:Curli are adhesive fimbriae of Enterobacteriaceae and are involved in surface attachment, cell aggregation, and biofilm formation. Here, we report that both inter- and intrastrain variations in curli production are widespread in enterohemorrhagic Escherichia coli O157:H7. The relative proportions of curli-producing va...

journal_title：Applied and environmental microbiology

authors： Carter MQ,Brandl MT,Louie JW,Kyle JL,Carychao DK,Cooley MB,Parker CT,Bates AH,Mandrell RE

更新日期：2011-06-01 00:00:00

abstract：:Controlling antibiotic-resistant (ART) bacteria in cheese fermentation is important for food safety and public health. A plant-maintained culture was found to be a potential source for ART bacterial contamination in cheese fermentation. Antibiotics had a detectable effect on the ART population from contamination in th...

journal_title：Applied and environmental microbiology

authors： Li X,Li Y,Alvarez V,Harper WJ,Wang HH

更新日期：2011-10-01 00:00:00

abstract：:RNA was degraded at 60 degrees C for 24 h by halophilic nuclease H in supernatants from broth cultures of Micrococcus varians subsp. halophilus containing 12% NaCl. Since contaminating 5'-nucleotidase exhibited almost no activity under these conditions, the 5'-GMP formed could be recovered from the reaction mixture, a...

journal_title：Applied and environmental microbiology

authors： Kamekura M,Hamakawa T,Onishi H

更新日期：1982-10-01 00:00:00

abstract：:Trihalomethanes, carbon tetrachloride, 1,1,1-trichloroethane, 1,2-dibromoethane, chlorinated benzenes, ethylbenzene, and naphthalene at concentrations commonly found in surface and groundwater were incubated under anoxic conditions to study their transformability in the presence of denitrifying bacteria. None of the a...

journal_title：Applied and environmental microbiology

authors： Bouwer EJ,McCarty PL

更新日期：1983-04-01 00:00:00