In situ production of crenarchaeol in two california hot springs.

Abstract:

:Crenarchaeol, a membrane-spanning glycerol dialkyl glycerol tetraether (GDGT) containing a cyclohexane moiety in addition to four cyclopentane moieties, was originally hypothesized to be synthesized exclusively by the mesophilic Crenarchaeota. Recent studies reporting the occurrence of crenarchaeol in hot springs and as a membrane constituent of the recently isolated thermophilic crenarchaeote "Candidatus Nitrosocaldus yellowstonii," however, have raised questions regarding its taxonomic distribution and function. To determine whether crenarchaeol in hot springs is indeed synthesized by members of the Archaea in situ or is of allochthonous origin, we quantified crenarchaeol present in the form of both intact polar lipids (IPLs) and core lipids in sediments of two California hot springs and in nearby soils. IPL-derived crenarchaeol (IPL-crenarchaeol) was found in both hot springs and soils, suggesting in situ production of this GDGT over a wide temperature range (12 degrees C to 89 degrees C). Quantification of archaeal amoA gene abundance by quantitative PCR showed a good correspondence with IPL-crenarchaeol, suggesting that it was indeed derived from living cells and that crenarchaeol-synthesizing members of the Archaea in our samples may also be ammonia oxidizers.

journal_name

Appl Environ Microbiol

authors

Pitcher A,Schouten S,Sinninghe Damsté JS

doi

10.1128/AEM.02591-08

subject

Has Abstract

pub_date

2009-07-01 00:00:00

pages

4443-51

issue

13

eissn

0099-2240

issn

1098-5336

pii

AEM.02591-08

journal_volume

75

pub_type

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