Urease-encoding genes in ammonia-oxidizing bacteria.

abstract：:A novel thermostable alcohol dehydrogenase (ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH). The gene, tk0845, which encodes an aldo-keto reductase, was heterologously expressed in Escherichia coli. The enzyme was found ...

journal_title：Applied and environmental microbiology

authors： Wu X,Zhang C,Orita I,Imanaka T,Fukui T,Xing XH

更新日期：2013-04-01 00:00:00

abstract：:Fenton reactions are believed to play important roles in wood degradation by brown rot fungi. In this context, the effect of tropolone (2-hydroxycyclohepta-2,4,6-trienone), a metal chelator, on wood degradation by Poria placenta was investigated. Tropolone (50 micro M) strongly inhibits fungal growth on malt agar, but...

journal_title：Applied and environmental microbiology

authors： Diouf PN,Delbarre N,Perrin D,Gérardin P,Rapin C,Jacquot JP,Gelhaye E

更新日期：2002-09-01 00:00:00

abstract：:The anoxygenic phototrophic bacterium Rhodopseudomonas palustris produces methane (CH4) from carbon dioxide (CO2) and hydrogen (H2) from protons (H+) when it expresses a variant form of molybdenum (Mo) nitrogenase that has two amino acid substitutions near its active site. We examined the influence of light energy and...

journal_title：Applied and environmental microbiology

authors： Zheng Y,Harwood CS

更新日期：2019-04-18 00:00:00

abstract：:The cyanobacterium Microcystis can produce microcystins, a family of toxins that are of major concern in water management. In several lakes, the average microcystin content per cell gradually declines from high levels at the onset of Microcystis blooms to low levels at the height of the bloom. Such seasonal dynamics m...

journal_title：Applied and environmental microbiology

authors： Kardinaal WE,Tonk L,Janse I,Hol S,Slot P,Huisman J,Visser PM

更新日期：2007-05-01 00:00:00

abstract：:Five Pseudomonas aeruginosa strains were tested for the utilization of 47 low-molecular-weight compounds as their sole sources of carbon and energy for growth at a concentration of 2.5 g/liter. Of these compounds, 31 to 35 were consumed. Growth experiments in tap water at 15 degrees C were carried out with one particu...

journal_title：Applied and environmental microbiology

authors： van der Kooij D,Oranje JP,Hijnen WA

更新日期：1982-11-01 00:00:00

abstract：:Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

journal_title：Applied and environmental microbiology

authors： Mahne I,Tiedje JM

更新日期：1995-03-01 00:00:00

abstract：:Plants are in constant contact with a community of soil biota that contains fungi ranging from pathogenic to symbiotic. A few studies have demonstrated a critical role of chemical communication in establishing highly specialized relationships, but the general role for root exudates in structuring the soil fungal commu...

journal_title：Applied and environmental microbiology

authors： Broeckling CD,Broz AK,Bergelson J,Manter DK,Vivanco JM

更新日期：2008-02-01 00:00:00

abstract：:By targeted deletion of the polyglutamate operon (pga) in Bacillus licheniformis F11, a derivative form, F11.1 (Δpga), was obtained that, along with lacking polyglutamate (PGA) formation, displayed enhanced proteolytic activities. The phenotypic properties were maintained in a strain in which the chiBA operon was addi...

journal_title：Applied and environmental microbiology

authors： Hoffmann K,Daum G,Köster M,Kulicke WM,Meyer-Rammes H,Bisping B,Meinhardt F

更新日期：2010-12-01 00:00:00

abstract：:We devised a new method (the dorayaki method) using marine agar under in situ pressures to isolate barophilic bacteria from the intestinal contents of three deep-sea fishes (two Coryphaenoides yaquinae samples and one Ilyophis sp. sample) retrieved from depths of 4,700 to 6,100 m in the Northwest Pacific Ocean. All 10...

journal_title：Applied and environmental microbiology

authors： Nakayama A,Yano Y,Yoshida K

更新日期：1994-11-01 00:00:00

abstract：:Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of gamma-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting diff...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Warén A,Orphan VJ,Van Dover CL,Vrijenhoek RC

更新日期：2004-05-01 00:00:00

abstract：:Relationships between the hyphae of Agaricus bisporus (Lang) Sing and bacteria from the mushroom bed casing layer were examined with a scanning electron microscope. Hyphae growing in the casing layer differed morphologically from compost-grown hyphae. Whereas the compost contained thin single hyphae surrounded by calc...

journal_title：Applied and environmental microbiology

authors： Masaphy S,Levanon D,Tchelet R,Henis Y

更新日期：1987-05-01 00:00:00

abstract：:A total of 85 isolates of mesophilic, aerobic, heterotrophic bacteria were isolated from the gut, peristomial membrane, and coelomic fluid from specimens of the sea urchin Echinus esculentus from the Clyde Sea area of Scotland. These isolates were compared with 26 isolates from sand and seawater in the same locality. ...

journal_title：Applied and environmental microbiology

authors： Unkles SE

更新日期：1977-10-01 00:00:00

abstract：:A Bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples. The organism was isolated on a glucose-mineral salts medium with phosphate deleted. Anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite conc...

journal_title：Applied and environmental microbiology

authors： Foster TL,Winans L Jr,Helms SJ

更新日期：1978-05-01 00:00:00

abstract：:Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soi...

journal_title：Applied and environmental microbiology

authors： Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

更新日期：2003-03-01 00:00:00

abstract：:Ergosterol is a membrane component specific to fungi that can be used to estimate fungal biomass using appropriate factors of conversion. Our objectives were to determine the limits of use of ergosterol content as a measure of biomass for aquatic hyphomycetes, and to evaluate a previously established ergosterol-to-bio...

journal_title：Applied and environmental microbiology

authors： Charcosset JY,Chauvet E

更新日期：2001-05-01 00:00:00

abstract：:Sodium gradients in cyanobacteria play an important role in energy storage under photoautotrophic conditions but have not been well studied during autofermentative metabolism under the dark, anoxic conditions widely used to produce precursors to fuels. Here we demonstrate significant stress-induced acceleration of aut...

journal_title：Applied and environmental microbiology

authors： Carrieri D,Ananyev G,Lenz O,Bryant DA,Dismukes GC

更新日期：2011-10-01 00:00:00

abstract：:The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...

journal_title：Applied and environmental microbiology

authors： Abe J,Ushijima C,Hizukuri S

更新日期：1999-09-01 00:00:00

abstract：:Large numbers of bacteriophages (2 x 10(7) to 1 x 10(8)/ml) were present in ruminal fluid from sheep and cattle. Twenty-six distinct types were identified and placed in three morphological groups; several phages possessed unusual structural features. The large numbers and diversity of phages observed indicates a possi...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Bauchop T

更新日期：1988-06-01 00:00:00

abstract：:The bacteria that cause necrotizing hepatopancreatitis in Penaeus vannamei adversely affect penaeid shrimp cultured in the western hemisphere. 16S rRNA and gyrase B gene analyses determined the taxonomic position of these bacteria. The name "Candidatus Hepatobacter penaei" is proposed for these pathogenic bacteria, wh...

journal_title：Applied and environmental microbiology

authors： Nunan LM,Pantoja CR,Gomez-Jimenez S,Lightner DV

更新日期：2013-02-01 00:00:00

abstract：:A mutant of Pseudomonas sp. strain HBP1, originally isolated on 2-hydroxybiphenyl, was selected for the ability to grow on 2-propylphenol as the sole carbon and energy source. In the mutant strain, which was designated as Pseudomonas sp. strain HBP1 Prp, the cellular induction mechanism involved in the synthesis of th...

journal_title：Applied and environmental microbiology

authors： Kohler HP,van der Maarel MJ,Kohler-Staub D

更新日期：1993-03-01 00:00:00

abstract：:The Arthrobacter sp. strain SU 4-chlorobenzoate (4-CBA) dehalogenation pathway converts 4-CBA to 4-hydroxybenzoate (4-HBA). The pathway operon contains the genes fcbA, fcbB, and fcbC (A. Schmitz, K. H. Gartemann, J. Fiedler, E. Grund, and R. Eichenlaub, Appl. Environ. Microbiol. 58:4068-4071, 1992). Genes fcbA and fcb...

journal_title：Applied and environmental microbiology

authors： Zhuang Z,Gartemann KH,Eichenlaub R,Dunaway-Mariano D

更新日期：2003-05-01 00:00:00

abstract：:Curli are adhesive fimbriae of Enterobacteriaceae and are involved in surface attachment, cell aggregation, and biofilm formation. Here, we report that both inter- and intrastrain variations in curli production are widespread in enterohemorrhagic Escherichia coli O157:H7. The relative proportions of curli-producing va...

journal_title：Applied and environmental microbiology

authors： Carter MQ,Brandl MT,Louie JW,Kyle JL,Carychao DK,Cooley MB,Parker CT,Bates AH,Mandrell RE

更新日期：2011-06-01 00:00:00

abstract：:In this study, a number of Listeria monocytogenes strains of different origins were evaluated for in vitro invasion capacity for various human cell types (monocytic THP-1, enterocytic Caco-2, and hepatocytic HepG2 cells) and for expression levels of specific virulence genes. For THP-1 cells, no differences between cli...

journal_title：Applied and environmental microbiology

authors： Werbrouck H,Grijspeerdt K,Botteldoorn N,Van Pamel E,Rijpens N,Van Damme J,Uyttendaele M,Herman L,Van Coillie E

更新日期：2006-06-01 00:00:00

abstract：:Cyclic lipopeptides (CLPs) are produced by many Pseudomonas species and have several biological functions, including a role in surface motility, biofilm formation, virulence, and antimicrobial activity. This study focused on the diversity and role of LuxR-type transcriptional regulators in CLP biosynthesis in Pseudomo...

journal_title：Applied and environmental microbiology

authors： de Bruijn I,Raaijmakers JM

更新日期：2009-07-01 00:00:00

abstract：:1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been consider...

journal_title：Applied and environmental microbiology

authors： Monincová M,Prokop Z,Vévodová J,Nagata Y,Damborsky J

更新日期：2007-03-01 00:00:00

abstract：:To investigate the biochemical basis of the differences in the insecticidal spectrum of Bacillus thuringiensis insecticidal crystal proteins (ICPs), we performed membrane binding and toxicity assays with three different ICPs and three lepidopteran species. The three ICPs have different toxicity patterns in the three s...

journal_title：Applied and environmental microbiology

authors： Van Rie J,Jansens S,Höfte H,Degheele D,Van Mellaert H

更新日期：1990-05-01 00:00:00

abstract：:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

journal_title：Applied and environmental microbiology

authors： Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

更新日期：2000-09-01 00:00:00

abstract：:ADP-glucose pyrophosphorylase (AGPase) and glycogen synthase (GS) catalyze the first two reactions of glycogen synthesis in cyanobacteria. Mutants defective in each of these enzymes in Synechococcus elongatus PCC 7942 were constructed and characterized. Activities of the corresponding enzymes in the selected mutants w...

journal_title：Applied and environmental microbiology

authors： Suzuki E,Ohkawa H,Moriya K,Matsubara T,Nagaike Y,Iwasaki I,Fujiwara S,Tsuzuki M,Nakamura Y

更新日期：2010-05-01 00:00:00

abstract：:Fusarium tricinctum Fn-2B was grown on a rice substrate at room temperature (22 to 26 degrees C) for 2 weeks followed by growth at a low temperature (10 to 12 degrees C) for an additional 2 weeks. A total of 1.5 g of nivalenol and 80 mg of fusarenone-X were obtained from 2 kg of rice culture. The methods of production...

journal_title：Applied and environmental microbiology

authors： Lee YW,Mirocha CJ

更新日期：1984-10-01 00:00:00

abstract：:One of the first steps in characterizing an ecosystem is to describe the organisms inhabiting it. For microbial studies, experimental limitations have hindered the ability to depict diverse communities. Here we describe oligonucleotide fingerprinting of rRNA genes (OFRG), a method that permits identification of arraye...

journal_title：Applied and environmental microbiology

authors： Valinsky L,Della Vedova G,Scupham AJ,Alvey S,Figueroa A,Yin B,Hartin RJ,Chrobak M,Crowley DE,Jiang T,Borneman J

更新日期：2002-07-01 00:00:00