Duplex PCR methods for the molecular detection of Escherichia fergusonii isolates from broiler chickens.

Abstract:

:Escherichia fergusonii is an emerging pathogen that has been isolated from a wide range of infections in animals and humans. Primers targeting specific genes, including yliE (encoding a conserved hypothetical protein of the cellulose synthase and regulator of cellulose synthase island), EFER_1569 (encoding a hypothetical protein, putative transcriptional activator for multiple antibiotic resistance), and EFER_3126 (encoding a putative triphosphoribosyl-dephospho-coenzyme A [CoA]), were designed for the detection of E. fergusonii by conventional and real-time PCR methods. Primers were screened by in silico PCR against 489 bacterial genomic sequences and by both PCR methods on 55 reference and field strains. Both methods were specific and sensitive for E. fergusonii, showing amplification only for this bacterium. Conventional PCR required a minimum bacterial concentration of approximately 10(2) CFU/ml, while real-time PCR required a minimum of 0.3 pg of DNA for consistent detection. Standard curves showed an efficiency of 98.5%, with an R(2) value of 0.99 for the real-time PCR assay. Cecal and cloacal contents from 580 chickens were sampled from broiler farms located in the Fraser Valley (British Columbia, Canada). Presumptive E. fergusonii isolates were recovered by enrichment and plating on differential and selective media. Of 301 total presumptive isolates, 140 (46.5%) were identified as E. fergusonii by biochemical profiling with the API 20E system and 268 (89.0%) using PCR methods. E. fergusonii detection directly from cecal and cloacal samples without preenrichment was achieved with both PCR methods. Hence, the PCR methods developed in this work significantly improve the detection of E. fergusonii.

journal_name

Appl Environ Microbiol

authors

Simmons K,Rempel H,Block G,Forgetta V,Vaillancourt R Jr,Malouin F,Topp E,Delaquis P,Diarra MS

doi

10.1128/AEM.04169-13

subject

Has Abstract

pub_date

2014-03-01 00:00:00

pages

1941-8

issue

6

eissn

0099-2240

issn

1098-5336

pii

AEM.04169-13

journal_volume

80

pub_type

杂志文章
  • Analysis of the genome sequence of Lactobacillus gasseri ATCC 33323 reveals the molecular basis of an autochthonous intestinal organism.

    abstract::This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. The plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. The GC content was 35.3...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00054-08

    authors: Azcarate-Peril MA,Altermann E,Goh YJ,Tallon R,Sanozky-Dawes RB,Pfeiler EA,O'Flaherty S,Buck BL,Dobson A,Duong T,Miller MJ,Barrangou R,Klaenhammer TR

    更新日期:2008-08-01 00:00:00

  • Biogeography of a Novel Ensifer meliloti Clade Associated with the Australian Legume Trigonella suavissima.

    abstract::Here, we describe a novel clade within Ensifer meliloti and consider how geographic and ecological isolation contributed to the limited distribution of this group. Members of the genus Ensifer are best known for their ability to form nitrogen-fixing symbioses with forage legumes of three related genera, Medicago L., M...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03446-16

    authors: Eardly B,Elia P,Brockwell J,Golemboski D,van Berkum P

    更新日期:2017-05-01 00:00:00

  • A new Klebsiella planticola strain (Cd-1) grows anaerobically at high cadmium concentrations and precipitates cadmium sulfide.

    abstract::Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of Na...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.7.3083-3087.2000

    authors: Sharma PK,Balkwill DL,Frenkel A,Vairavamurthy MA

    更新日期:2000-07-01 00:00:00

  • Cloning and nucleotide sequence of the gene coding for aspartokinase II from a thermophilic methylotrophic Bacillus sp.

    abstract::The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.9.2806-2814.1992

    authors: Schendel FJ,Flickinger MC

    更新日期:1992-09-01 00:00:00

  • Assessing Transmission of Antimicrobial-Resistant Escherichia coli in Wild Giraffe Contact Networks.

    abstract::There is growing evidence that anthropogenic sources of antibiotics and antimicrobial-resistant bacteria can spill over into natural ecosystems, raising questions about the role wild animals play in the emergence, maintenance, and dispersal of antibiotic resistance genes. In particular, we lack an understanding of how...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02136-18

    authors: Miller EA,Johnson TJ,Omondi G,Atwill ER,Isbell LA,McCowan B,VanderWaal K

    更新日期:2018-12-13 00:00:00

  • Tremorgenic mycotoxin from Penicillium paraherquei.

    abstract::A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.3.441-442.1976

    authors: Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

    更新日期:1976-09-01 00:00:00

  • Interactions of Plutonium with Pseudomonas sp. Strain EPS-1W and Its Extracellular Polymeric Substances.

    abstract::Safe and effective nuclear waste disposal, as well as accidental radionuclide releases, necessitates our understanding of the fate of radionuclides in the environment, including their interaction with microorganisms. We examined the sorption of Pu(IV) and Pu(V) to Pseudomonas sp. strain EPS-1W, an aerobic bacterium is...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02572-16

    authors: Boggs MA,Jiao Y,Dai Z,Zavarin M,Kersting AB

    更新日期:2016-11-21 00:00:00

  • Biocatalyst engineering by assembly of fatty acid transport and oxidation activities for In vivo application of cytochrome P-450BM-3 monooxygenase.

    abstract::The application of whole cells containing cytochrome P-450BM-3 monooxygenase [EC 1.14.14.1] for the bioconversion of long-chain saturated fatty acids to omega-1, omega-2, and omega-3 hydroxy fatty acids was investigated. We utilized pentadecanoic acid and studied its conversion to a mixture of 12-, 13-, and 14-hydroxy...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.3784-3790.1998

    authors: Schneider S,Wubbolts MG,Sanglard D,Witholt B

    更新日期:1998-10-01 00:00:00

  • Binding of virus-like particles of Norwalk virus to romaine lettuce veins.

    abstract::Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01566-10

    authors: Gandhi KM,Mandrell RE,Tian P

    更新日期:2010-12-01 00:00:00

  • NAH plasmid-mediated catabolism of anthracene and phenanthrene to naphthoic acids.

    abstract::Pseudomonas fluorescens 5R contains an NAH7-like plasmid (pKA1), and P. fluorescens 5R mutant 5RL contains a bioluminescent reporter plasmid (pUTK21) which was constructed by transposon mutagenesis. Polymerase chain reaction mapping confirmed the localization of lux transposon Tn4431 300 bp downstream from the start o...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.59.6.1938-1942.1993

    authors: Menn FM,Applegate BM,Sayler GS

    更新日期:1993-06-01 00:00:00

  • Heterologous expression of polycyclic aromatic hydrocarbon ring-hydroxylating dioxygenase genes from a novel pyrene-degrading betaproteobacterium.

    abstract::A betaproteobacterium within the family Rhodocyclaceae previously identified as a pyrene degrader via stable-isotope probing (SIP) of contaminated soil (designated pyrene group 1 or PG1) was cultivated as the dominant member of a mixed bacterial culture. A metagenomic library was constructed, and the largest contigs w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00173-12

    authors: Singleton DR,Hu J,Aitken MD

    更新日期:2012-05-01 00:00:00

  • Isolation of Vibrio cholerae serotype O1 from the eastern oyster, Crassostrea virginica.

    abstract::Two strains of Vibrio cholerae serotype O1 Inaba were isolated from eastern oysters, Crassostrea virginica, collected from estuarine waters in Florida during April 1980. The oyster meats and waters from which the oysters were collected had low fecal coliform counts, and the area had no prior evidence of sewage contami...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.2.559-560.1981

    authors: Hood MA,Ness GE,Rodrick GE

    更新日期:1981-02-01 00:00:00

  • Evidence from Serpula lacrymans that 2,5-dimethoxyhydroquinone Is a lignocellulolytic agent of divergent brown rot basidiomycetes.

    abstract::Basidiomycetes that cause brown rot of wood are essential biomass recyclers in coniferous forest ecosystems and a major cause of failure in wooden structures. Recent work indicates that distinct lineages of brown rot fungi have arisen independently from ligninolytic white rot ancestors via loss of lignocellulolytic en...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03880-12

    authors: Korripally P,Timokhin VI,Houtman CJ,Mozuch MD,Hammel KE

    更新日期:2013-04-01 00:00:00

  • Purification of Thermotoga maritima enzymes for the degradation of cellulosic materials.

    abstract::A separation procedure for the analysis of the enzyme components of the hyperthermophilic bacterium Thermotoga maritima involved in cellulose and xylan degradation was developed. Resolution of the enzymes was achieved by a combination of fast protein liquid chromatography anion exchange and hydrophobic interaction chr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.4.1399-1407.1995

    authors: Bronnenmeier K,Kern A,Liebl W,Staudenbauer WL

    更新日期:1995-04-01 00:00:00

  • Plasmid-Associated Bacteriocin Production and Sucrose Fermentation in Pediococcus acidilactici.

    abstract::Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subs...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2534-2538.1987

    authors: Gonzalez CF,Kunka BS

    更新日期:1987-10-01 00:00:00

  • Respiration and growth of Shewanella decolorationis S12 with an Azo compound as the sole electron acceptor.

    abstract::The ability of Shewanella decolorationis S12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. This microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or H(2) as the electron donor. Furthermore, strain S12 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01415-06

    authors: Hong Y,Xu M,Guo J,Xu Z,Chen X,Sun G

    更新日期:2007-01-01 00:00:00

  • Competitive exclusion of Salmonella enterica serovar Enteritidis by Lactobacillus crispatus and Clostridium lactatifermentans in a sequencing fed-batch culture.

    abstract::Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.2.555-559.2002

    authors: van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

    更新日期:2002-02-01 00:00:00

  • Bile salt hydrolase of Bifidobacterium longum-biochemical and genetic characterization.

    abstract::A bile salt hydrolase (BSH) was isolated from Bifidobacterium longum SBT2928, purified, and characterized. Furthermore, we describe for the first time cloning and analysis of the gene encoding BSH (bsh) in a member of the genus Bifidobacterium. The enzyme has a native molecular weight of 125,000 to 130,000 and a subun...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.6.2502-2512.2000

    authors: Tanaka H,Hashiba H,Kok J,Mierau I

    更新日期:2000-06-01 00:00:00

  • Effects of acid adaptation, product pH, and heating on survival of Escherichia coli O157:H7 in pepperoni.

    abstract::The thermotolerance of E. coli O157:H7 cells (strain 380-94) heated in pepperoni is reported. Information on the pattern of thermal inactivation of E. coli O157:H7 in pepperoni was applied in the development of heating processes designed to reduce E. coli O157:H7 numbers therein by 5 log(10) units. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.4.1726-1729.2000

    authors: Riordan DC,Duffy G,Sheridan JJ,Whiting RC,Blair IS,McDowell DA

    更新日期:2000-04-01 00:00:00

  • Impact of Classical Strain Improvement of Penicillium rubens on Amino Acid Metabolism during β-Lactam Production.

    abstract::To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01561-19

    authors: Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

    更新日期:2020-01-21 00:00:00

  • Isolation and characterization of a fluoranthene-utilizing strain of Pseudomonas paucimobilis.

    abstract::A soil bacterium capable of utilizing fluoranthene as the sole source of carbon and energy for growth was purified from a seven-member bacterial community previously isolated from a creosote waste site for its ability to degrade polycyclic aromatic hydrocarbons. By standard bacteriological methods, this bacterium was ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.4.1079-1086.1990

    authors: Mueller JG,Chapman PJ,Blattmann BO,Pritchard PH

    更新日期:1990-04-01 00:00:00

  • Detection of microbial growth on polycyclic aromatic hydrocarbons in microtiter plates by using the respiration indicator WST-1.

    abstract::We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.6.2683-2689.2002

    authors: Johnsen AR,Bendixen K,Karlson U

    更新日期:2002-06-01 00:00:00

  • The motility symbiont of the termite gut flagellate Caduceia versatilis is a member of the "Synergistes" group.

    abstract::The flagellate Caduceia versatilis in the gut of the termite Cryptotermes cavifrons reportedly propels itself not by its own flagella but solely by the flagella of ectosymbiotic bacteria. Previous microscopic observations have revealed that the motility symbionts are flagellated rods partially embedded in the host cel...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00750-07

    authors: Hongoh Y,Sato T,Dolan MF,Noda S,Ui S,Kudo T,Ohkuma M

    更新日期:2007-10-01 00:00:00

  • Bioconversion of 7-Aminocephalosporanic Acid by Intact Rhodotorula glutinis Cells.

    abstract::When 7-aminocephalosporanic acid (7-ACA) was used as a single carbon source in the enrichment culture medium for screening 7-ACA-degrading microorganisms, pink yeast colonies appeared frequently, and these were identified as Rhodotorula glutinis. These intact R. glutinis cells converted (i) 7-ACA to deacetyl-7-ACA (7-...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.7.2669-2672.1996

    authors: Sakai Y,Abe T,Ohbayashi Y,Isaka K,Yamamoto K,Tani Y,Kato N

    更新日期:1996-07-01 00:00:00

  • Fusion of self-assembling amphipathic oligopeptides with cyclodextrin glycosyltransferase improves 2-O-D-glucopyranosyl-L-ascorbic acid synthesis with soluble starch as the glycosyl donor.

    abstract::In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors. The resul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01249-14

    authors: Han R,Li J,Shin HD,Chen RR,Liu L,Du G,Chen J

    更新日期:2014-08-01 00:00:00

  • Degradation of mono-, di-, and trihalogenated benzoic acids by Pseudomonas aeruginosa JB2.

    abstract::Pseudomonas aeruginosa JB2 was isolated from a polychlorinated biphenyl-contaminated soil by enrichment culture containing 2-chlorobenzoate as the sole carbon source. Strain JB2 was subsequently found also to grow on 3-chlorobenzoate, 2,3- and 2,5-dichlorobenzoates, 2,3,5-trichlorobenzoate, and a wide range of other m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.12.3842-3850.1990

    authors: Hickey WJ,Focht DD

    更新日期:1990-12-01 00:00:00

  • Development of a modified gentamicin resistance cassette for genetic manipulation of the oral spirochete Treponema denticola.

    abstract::Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07461-11

    authors: Bian J,Fenno JC,Li C

    更新日期:2012-03-01 00:00:00

  • Development of a Defined Medium for Growth of Cercospora rosicola Passerini.

    abstract::A simple synthetic liquid medium containing a single amino acid, glucose, salts, trace metals, and thiamine was developed for cultivation of Cercospora rosicola Passerini. Thiamine was shown to be important to growth. Culture of C. rosicola Passerini in a chemically defined medium makes possible studies of (+)-abscisi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.41.1.334-336.1981

    authors: Norman SM,Maier VP,Echols LC

    更新日期:1981-01-01 00:00:00

  • Bacillus amyloliquefaciens ALB65 Inhibits the Growth of Listeria monocytogenes on Cantaloupe Melons.

    abstract::Listeria monocytogenes is a foodborne pathogen that causes high rates of hospitalization and mortality in people infected. Contamination of fresh, ready to eat produce by this pathogen is especially troubling because of the ability of this bacterium to grow on produce under refrigeration temperatures. In this study, w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01926-20

    authors: Tran TD,Del Cid C,Hnasko R,Gorski L,McGarvey JA

    更新日期:2020-12-17 00:00:00

  • Production of nitrous oxide by ammonia-oxidizing chemoautotrophic microorganisms in soil.

    abstract::Gas chromatographic studies showed that nitrous oxide was produced in each instance when sterilized (autoclaved) soil was incubated after treatment with ammonium sulfate and inoculation with pure cultures of ammonia-oxidizing chemoautotrophic microorganisms (strains of Nitrosomonas, Nitrosospira, and Nitrosolobus). Pr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.6.1060-1066.1980

    authors: Blackmer AM,Bremner JM,Schmidt EL

    更新日期:1980-12-01 00:00:00