Viability PCR, a culture-independent method for rapid and selective quantification of viable Legionella pneumophila cells in environmental water samples.

abstract：:N-acylhomoserine lactones (AHLs) are signaling molecules in many quorum-sensing (QS) systems that regulate interactions between various pathogenic bacteria and their hosts. Quorum quenching by the enzymatic inactivation of AHLs holds great promise in preventing and treating infections, and several such enzymes have be...

journal_title：Applied and environmental microbiology

authors： Mei GY,Yan XX,Turak A,Luo ZQ,Zhang LQ

更新日期：2010-08-01 00:00:00

abstract：:The effects of various nitrogen sources and of the cultivation time on the biosynthesis of N-labelled azoverdin were studied in batch cultures of Azomonas macrocytogenes ATCC 12334. Growth for 48 h in the presence of ammonium sulfate (0.2 g/liter) resulted in 95% labelling of azoverdin with N. A growth-related synthes...

journal_title：Applied and environmental microbiology

authors： Dobisová M,Abdallah MA,Kyslík P

更新日期：1994-12-01 00:00:00

abstract：:Halophenols such as 2,4-dibromophenol (DBP) occur naturally in some marine sediments, as a consequence of various animal and algal activities. In an earlier study, DBP was observed in the burrow microenvironment of the hemichordate Saccoglossus kowalewskii. At the concentrations found in the burrow lining, aerobic res...

journal_title：Applied and environmental microbiology

authors： King GM

更新日期：1988-12-01 00:00:00

abstract：:Extracellular DNA (eDNA) is a biofilm component that contributes to the formation and structural stability of biofilms. Streptococcus mutans, a major cariogenic bacterium, induces eDNA-dependent biofilm formation under specific conditions. Since cell death can result in the release and accumulation of DNA, the dead ce...

journal_title：Applied and environmental microbiology

authors： Nagasawa R,Yamamoto T,Utada AS,Nomura N,Obana N

更新日期：2020-11-10 00:00:00

abstract：:To further study mechanisms of coping with osmotic stress-low water activity, mutants of Staphylococcus aureus with transposon Tn917-lacZ-induced NaCl sensitivity were selected for impaired ability to grow on solid defined medium containing 2 M NaCl. Southern hybridization experiments showed that NaCl-sensitive mutant...

journal_title：Applied and environmental microbiology

authors： Vijaranakul U,Nadakavukaren MJ,Bayles DO,Wilkinson BJ,Jayaswal RK

更新日期：1997-05-01 00:00:00

abstract：:Pseudomonas aureofaciens PGS12 produces three phenazine antibiotics, in addition to siderophores, hydrogen cyanide, pyrrolnitrin, and indoleacetic acid. Tn5-259.7 transposon mutagenesis was carried out to identify and clone a chromosomal locus involved in phenazine biosynthesis. Three classes of mutants were obtained:...

journal_title：Applied and environmental microbiology

authors： Georgakopoulos DG,Hendson M,Panopoulos NJ,Schroth MN

更新日期：1994-08-01 00:00:00

abstract：:This study measured the environmental variability which exists in a commercial aerobic composting process. The specific process studied is carried out in six decomposition cells which present six different phases of the process. Temperature, pH, and moisture content were determined in several randomly chosen sample si...

journal_title：Applied and environmental microbiology

authors： Atchley SH,Clark JB

更新日期：1979-12-01 00:00:00

abstract：:We examined the effect of different sediment types on the staining effectiveness of the fluorochrome DAPI (4'-6-diamidino-2-phenylindole dihydrochloride) over a wide range of concentrations and on the masking effect of sediment particles on DAPI-stained bacteria. Sediment type greatly affects the staining efficiency o...

journal_title：Applied and environmental microbiology

authors： Schallenberg M,Kalff J,Rasmussen JB

更新日期：1989-05-01 00:00:00

abstract：:To investigate the effects of land use and crop management on soybean rhizobial communities, 280 nodule isolates were trapped from 7 fields with different land use and culture histories. Besides the known Bradyrhizobium japonicum, three novel genospecies were isolated from these fields. Grassland (GL) maintained a hig...

journal_title：Applied and environmental microbiology

authors： Yan J,Han XZ,Ji ZJ,Li Y,Wang ET,Xie ZH,Chen WF

更新日期：2014-09-01 00:00:00

abstract：:The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and bio...

journal_title：Applied and environmental microbiology

authors： Dejonghe W,Berteloot E,Goris J,Boon N,Crul K,Maertens S,Höfte M,De Vos P,Verstraete W,Top EM

更新日期：2003-03-01 00:00:00

abstract：:Wastewater disinfection is used in many countries for reducing fecal coliform levels in effluents. Disinfection is therefore frequently used to improve recreational bathing waters which do not comply with microbiological standards. It is unknown whether human enteric viruses (which are responsible for waterborne disea...

journal_title：Applied and environmental microbiology

authors： Tree JA,Adams MR,Lees DN

更新日期：2003-04-01 00:00:00

abstract：:Fermentation strategies for the production of poly(3-hydroxybutyrate) (PHB) from whey by recombinant Escherichia coli strain CGSC 4401 harboring the Alcaligenes latus polyhydroxyalkanoate (PHA) biosynthesis genes were developed. The pH-stat fed-batch cultures of E. coli CGSC 4401 harboring pJC4, a stable plasmid conta...

journal_title：Applied and environmental microbiology

authors： Ahn WS,Park SJ,Lee SY

更新日期：2000-08-01 00:00:00

abstract：:A separation procedure for the analysis of the enzyme components of the hyperthermophilic bacterium Thermotoga maritima involved in cellulose and xylan degradation was developed. Resolution of the enzymes was achieved by a combination of fast protein liquid chromatography anion exchange and hydrophobic interaction chr...

journal_title：Applied and environmental microbiology

authors： Bronnenmeier K,Kern A,Liebl W,Staudenbauer WL

更新日期：1995-04-01 00:00:00

abstract：:Cyanobacterial hepatotoxins, microcystins, are specific inhibitors of serine/threonine protein phosphatases and potent tumor promoters. They have caused several poisonings of animals and also pose a health hazard for humans through the use of water for drinking and recreation. Different strains of the same cyanobacter...

journal_title：Applied and environmental microbiology

authors： Rapala J,Sivonen K,Lyra C,Niemelä SI

更新日期：1997-06-01 00:00:00

abstract：:Wolbachia, a common bacterial endosymbiont of insects, has been shown to protect its hosts against a wide range of pathogens. However, not all strains exert a protective effect on their host. Here we assess the effects of two divergent Wolbachia strains, wAlbB from Aedes albopictus and wMelPop from Drosophila melanoga...

journal_title：Applied and environmental microbiology

authors： Hughes GL,Vega-Rodriguez J,Xue P,Rasgon JL

更新日期：2012-03-01 00:00:00

abstract：:White rot fungi can oxidize high-molecular-weight polycyclic aromatic hydrocarbons (PAH) rapidly to polar metabolites, but only limited mineralization takes place. The objectives of this study were to determine if the polar metabolites can be readily mineralized by indigenous microflora from several inoculum sources, ...

journal_title：Applied and environmental microbiology

authors： Kotterman MJ,Vis EH,Field JA

更新日期：1998-08-01 00:00:00

abstract：:A compound required for the luxuriant growth of Lactobacillus sanfrancisco in laboratory media was isolated from a freshly prepared yeast extract, purified, and characterized. The stimulant was identified as a small peptide with a molecular weight of approximately 1,065, containing aspartic acid, cysteine, glutamic ac...

journal_title：Applied and environmental microbiology

authors： Berg RW,Sandine WE,Anderson AW

更新日期：1981-11-01 00:00:00

abstract：:This study presents population analyses of microbial communities inhabiting a site of extreme acid mine drainage (AMD) production. The site is the inactive underground Richmond mine at Iron Mountain, Calif., where the weathering of a massive sulfide ore body (mostly pyrite) produces solutions with pHs of approximately...

journal_title：Applied and environmental microbiology

authors： Bond PL,Druschel GK,Banfield JF

更新日期：2000-11-01 00:00:00

abstract：:Juvenile oyster disease (JOD) causes significant annual mortalities of hatchery-produced Eastern oysters, Crassostrea virginica, cultured in the Northeast. We have reported that a novel species of the alpha-proteobacteria Roseobacter group (designated CVSP) was numerically dominant in JOD-affected animals sampled duri...

journal_title：Applied and environmental microbiology

authors： Boettcher KJ,Barber BJ,Singer JT

更新日期：2000-09-01 00:00:00

abstract：:Laboratory studies of methane formation in peat samples from an acid subarctic mire in Sweden indicated the presence of a low-temperature-adapted methanogenic flora. Enrichment culture studies with ethanol, acetate, hydrogen, or a combination of these as substrate for methane formation provided evidence for the existe...

journal_title：Applied and environmental microbiology

authors： Svensson BH

更新日期：1984-08-01 00:00:00

abstract：:We determined the effects of grain size and nutritional conditions on the penetration rate and metabolic activity of Escherichia coli strains in anaerobic, nutrient-saturated chambers packed with different sizes of glass beads (diameters, 116 to 767 mum) under static conditions. The chambers had nearly equal porositie...

journal_title：Applied and environmental microbiology

authors： Sharma PK,McInerney MJ

更新日期：1994-05-01 00:00:00

abstract：:A total of four pathways are known for the catabolism by microorganisms of gentisate (2,5-dihydroxybenzoate) and homogentisate (2,5-dihydroxyphenylacetate). Both of these dihydric phenols can be degraded by either a glutathione-dependent or a glutathione-independent reaction sequence. We found that it is not always po...

journal_title：Applied and environmental microbiology

authors： Crawford RL,Frick TD

更新日期：1977-08-01 00:00:00

abstract：:Flavobacterium psychrophilum, a member of the Cytophaga-Flavobacterium-Bacteroides group, is an important pathogen of salmonid fish. Previous attempts to develop genetic techniques for this fastidious, psychrotrophic bacterium have met with failure. Here we describe the development of techniques for the genetic manipu...

journal_title：Applied and environmental microbiology

authors： Alvarez B,Secades P,McBride MJ,Guijarro JA

更新日期：2004-01-01 00:00:00

abstract：:Lactic acid bacteria have a good potential as agents for the delivery of heterologous proteins to the gastrointestinal mucosa and thus for the reequilibration of inappropriate immune responses to food antigens. Bovine beta-lactoglobulin (BLG) is considered a major allergen in cow's milk allergy. We have designed recom...

journal_title：Applied and environmental microbiology

authors： Bernasconi E,Germond JE,Delley M,Fritsché R,Corthésy B

更新日期：2002-06-01 00:00:00

abstract：:Growth of Zymomonas mobilis ATCC 29191 and CP4 in a continuous stirred tank fermentor resulted in the selection of stable flocculating variants. Factors responsible for enhancing the system pressures selective for the morphological variants were identified. By incorporating some modifications into the design of the fe...

journal_title：Applied and environmental microbiology

authors： Fein JE,Zawadzki BC,Lawford HG,Lawford GR

更新日期：1983-06-01 00:00:00

abstract：:To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene ...

journal_title：Applied and environmental microbiology

authors： Zhang C,Rausch D,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:The cellulosomes produced by Clostridium cellulovorans are organized by the specific interactions between the cohesins in the scaffolding proteins and the dockerins of the catalytic components. Using a cohesin biomarker, we identified a cellulosomal enzyme which belongs to the glycosyl hydrolase family 5 and has a dom...

journal_title：Applied and environmental microbiology

authors： Jeon SD,Lee JE,Kim SJ,Park SH,Choi GW,Han SO

更新日期：2013-10-01 00:00:00

abstract：:We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background bu...

journal_title：Applied and environmental microbiology

authors： Charles TC,Doty SL,Nester EW

更新日期：1994-11-01 00:00:00

abstract：:We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL...

journal_title：Applied and environmental microbiology

authors： Joseph-Horne T,Hollomon D,Manning N,Kelly SL

更新日期：1996-01-01 00:00:00

abstract：:Transpositional mutagenesis of Thiobacillus novellus by Tn501 was achieved by means of the incompatibility of IncP plasmids. Tn501 insertion caused three types of mutant phenotypes: isoleucine auxotrophy, lysine auxotrophy, and a reduced ability to oxidize reduced sulfur compounds and to fix CO(2). Oxidation rates for...

journal_title：Applied and environmental microbiology

authors： Davidson MS,Roy P,Summers AO

更新日期：1985-06-01 00:00:00