Solutions to problems in enumerating sediment bacteria by direct counts.

abstract：:Nested PCR with three highly degenerate primers was used for amplification and identification of DNA polymerase (pol) genes from viruses which infect three genera of microalgae. Group-specific primers (AVS1 and AVS2) were designed on the basis of inferred amino acid sequences unique to the DNA pol genes of viruses (PB...

journal_title：Applied and environmental microbiology

authors： Chen F,Suttle CA

更新日期：1995-04-01 00:00:00

abstract：:Four Staphylococcus aureus-Escherichia coli shuttle vectors were constructed for gene expression and production of tagged fusion proteins. Vectors pBUS1-HC and pTSSCm have no promoter upstream of the multiple cloning site (MCS), and this allows study of genes under the control of their native promoters, and pBUS1-Pcap...

journal_title：Applied and environmental microbiology

authors： Schwendener S,Perreten V

更新日期：2015-05-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

journal_title：Applied and environmental microbiology

authors： Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

更新日期：2007-10-01 00:00:00

abstract：:We used global transcript analyses and mutant studies to investigate the pathways that impact H(2) production in the photosynthetic bacterium Rhodobacter sphaeroides. We found that H(2) production capacity is related to the levels of expression of the nitrogenase and hydrogenase enzymes and the enzymes of the Calvin-B...

journal_title：Applied and environmental microbiology

authors： Kontur WS,Ziegelhoffer EC,Spero MA,Imam S,Noguera DR,Donohue TJ

更新日期：2011-10-01 00:00:00

abstract：:A fragment of about 500 base pairs of the beta-hemolysin gene from Listeria monocytogenes was used to screen different bacterial strains by DNA colony hybridization. The cells in the colonies were lysed by microwaves in the presence of sodium hydroxide. Of 52 different strains of Listeria species screened, only the DN...

journal_title：Applied and environmental microbiology

authors： Datta AR,Wentz BA,Hill WE

更新日期：1987-09-01 00:00:00

abstract：:The inactivation of catechol 2,3-dioxygenase from Pseudomonas putida mt-2 by 3-chloro- and 3-fluorocatechol and the iron-chelating agent Tiron (catechol-3,5-disulfonate) was studied. Whereas inactivation by Tiron is an oxygen-independent and mostly reversible process, inactivation by the 3-halocatechols was only obser...

journal_title：Applied and environmental microbiology

authors： Bartels I,Knackmuss HJ,Reineke W

更新日期：1984-03-01 00:00:00

abstract：:The production of pectolytic enzymes (pectate lyase [Pel] and polygalacturonase [Peh]), cellulase (Cel), and protease (Prt) is activated in the soft rot bacterium Erwinia carotovora subsp. carotovora by aepA (activator of extracellular protein production) and celery extract (Y. Liu, H. Murata, A. Chatterjee, and A. K....

journal_title：Applied and environmental microbiology

authors： Murata H,Chatterjee A,Liu Y,Chatterjee AK

更新日期：1994-09-01 00:00:00

abstract：:In the present work, we aimed at determining the relationship between the hepatitis A virus (HAV) numbers in imported frozen coquina clams involved in two hepatitis outbreaks, as well as the risk for human health. Due to HAV unculturability, a standardized TaqMan real-time reverse transcription-PCR controlling the vir...

journal_title：Applied and environmental microbiology

authors： Pintó RM,Costafreda MI,Bosch A

更新日期：2009-12-01 00:00:00

abstract：:We developed an improved method for cultivation-independent sorting of bacterial cells. The technique is based on labeling the target cells by in situ hybridization with polynucleotide transcript probes. Due to the probes' length, part of the probe remains outside the cell and can subsequently be used to capture the c...

journal_title：Applied and environmental microbiology

authors： Zwirglmaier K,Ludwig W,Schleifer KH

更新日期：2004-01-01 00:00:00

abstract：:The effects of aflatoxin on egg production, fertility, hatchability, and progeny performance were investigated by feeding dietary aflatoxin at dose levels of 0,5, and 10 mug/g to mature broiler breeder hens for 4 weeks. Sixteen hens were used for each dietary dose level. Egg production decreased significantly during w...

journal_title：Applied and environmental microbiology

authors： Howarth B Jr,Wyatt RD

更新日期：1976-05-01 00:00:00

abstract：:Plasmid constructs pNW1 through pNW6 containing a controllable xylE gene (for catechol 2,3-dioxygenase) were introduced into Streptomyces lividans strains to provide a selectable marker system. xylE functions in S. lividans under the control of bacteriophage lambda promoters lambda pL and lambda pR. Thermoregulated ex...

journal_title：Applied and environmental microbiology

authors： Wipat A,Wellington EM,Saunders VA

更新日期：1991-11-01 00:00:00

abstract：:The pH and the nature an concentration of simple electrolytes influenced the interaction of poliovirus type 2 with three soils, a sand, and a clay mineral. In electrolytes above pH 9 the virus was not adsorbed extensively to the substrates, but below pH 7 almost all virus was bound. For each adsorbent there was a char...

journal_title：Applied and environmental microbiology

authors： Taylor DH,Moore RS,Sturman LS

更新日期：1981-12-01 00:00:00

abstract：:We describe useful vectors to select double-crossover events directly in site-directed marker exchange mutagenesis in gram-negative bacteria. These vectors contain the gusA marker gene, providing colorimetric screens to identify bacteria harboring those sequences. The applicability of these vectors was shown by mappin...

journal_title：Applied and environmental microbiology

authors： Katzen F,Becker A,Ielmini MV,Oddo CG,Ielpi L

更新日期：1999-01-01 00:00:00

abstract：:Rsan-ver, a strain of Pseudomonas aeruginosa isolated at this department, was used for the development of a continuous process for biosurfactant production. The active compounds were identified as rhamnolipids. A final medium for production was designed in continuous culture by means of medium shifts, since the format...

journal_title：Applied and environmental microbiology

authors： Guerra-Santos L,Käppeli O,Fiechter A

更新日期：1984-08-01 00:00:00

abstract：:Restriction fragment length polymorphism (RFLP) and vegetative compatibility analyses were undertaken to assess genetic relationships among 52 isolates of Fusarium oxysporum f. sp. vasinfectum of worldwide origin and representing race A, 3, or 4 on cotton plants. Ten distinct vegetative compatibility groups (VCGs) wer...

journal_title：Applied and environmental microbiology

authors： Fernandez D,Assigbese K,Dubois MP,Geiger JP

更新日期：1994-11-01 00:00:00

abstract：:We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...

journal_title：Applied and environmental microbiology

authors： Liles MR,Williamson LL,Rodbumrer J,Torsvik V,Goodman RM,Handelsman J

更新日期：2008-05-01 00:00:00

abstract：:The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrog...

journal_title：Applied and environmental microbiology

authors： Cotta SR,Dias AC,Marriel IE,Andreote FD,Seldin L,van Elsas JD

更新日期：2014-10-01 00:00:00

abstract：:Differences between the recombinant xylose-utilizing Saccharomyces cerevisiae strain TMB 3399 and the mutant strain TMB 3400, derived from TMB 3399 and displaying improved ability to utilize xylose, were investigated by using genome-wide expression analysis, physiological characterization, and biochemical assays. Samp...

journal_title：Applied and environmental microbiology

authors： Wahlbom CF,Cordero Otero RR,van Zyl WH,Hahn-Hägerdal B,Jönsson LJ

更新日期：2003-02-01 00:00:00

abstract：:The extensive use of antibiotics in hospitals and in the animal breeding industry has promoted antibiotic resistance in bacteria, which resulted in the emergence of a large number of antibiotic resistance genes in the intestinal tract of human and farmed animals. Genetic exchange of resistance genes between the two ec...

journal_title：Applied and environmental microbiology

authors： Wang C,Song Y,Tang N,Zhang G,Leclercq SO,Feng J

更新日期：2020-12-11 00:00:00

abstract：:Swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. This study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions...

journal_title：Applied and environmental microbiology

authors： Abdelzaher AM,Wright ME,Ortega C,Solo-Gabriele HM,Miller G,Elmir S,Newman X,Shih P,Bonilla JA,Bonilla TD,Palmer CJ,Scott T,Lukasik J,Harwood VJ,McQuaig S,Sinigalliano C,Gidley M,Plano LR,Zhu X,Wang JD,Fleming LE

更新日期：2010-02-01 00:00:00

abstract：:A clinical isolate of Pseudomonas aeruginosa was found capable of utilizing salicylate by the salicylate hydroxylase and beta-ketoadipate pathway. ...

journal_title：Applied and environmental microbiology

authors： Ohta S,Matsumoto H,Terawaki Y

更新日期：1981-01-01 00:00:00

abstract：:The adherent-invasive Escherichia coli (AIEC) pathotype, which has been associated with Crohn's disease, shows similar traits to human and animal extraintestinal pathogenic E. coli (ExPEC) with respect to their phylogenetic origin and virulence gene profiles. Here, we demonstrate that animal ExPEC strains generally do...

journal_title：Applied and environmental microbiology

authors： Martinez-Medina M,Garcia-Gil J,Barnich N,Wieler LH,Ewers C

更新日期：2011-08-15 00:00:00

abstract：:Macroalgae (or seaweeds) are considered potential biomass feedstocks for the production of renewable fuels and chemicals. Their sugar composition is different from that of lignocellulosic biomasses, and in green species, including Ulva lactuca, the major sugars are l-rhamnose and d-glucose. C. beijerinckii DSM 6423 ut...

journal_title：Applied and environmental microbiology

authors： Diallo M,Simons AD,van der Wal H,Collas F,Houweling-Tan B,Kengen SWM,López-Contreras AM

更新日期：2019-02-20 00:00:00

abstract：:A novel thermostable alcohol dehydrogenase (ADH) showing activity toward aromatic secondary alcohols was identified from the hyperthermophilic archaeon Thermococcus kodakarensis KOD1 (TkADH). The gene, tk0845, which encodes an aldo-keto reductase, was heterologously expressed in Escherichia coli. The enzyme was found ...

journal_title：Applied and environmental microbiology

authors： Wu X,Zhang C,Orita I,Imanaka T,Fukui T,Xing XH

更新日期：2013-04-01 00:00:00

abstract：:DNA microarray analysis showed that yfiD, yggB, and yggE genes were up-regulated when superoxide dismutase (SOD)-deficient Escherichia coli IM303 (I4) was cultivated under the oxidative stress generated by photoexcited TiO(2), and pYFD, pYGB, and pYGE were constructed by inserting the respective genes into a pUC 19 ve...

journal_title：Applied and environmental microbiology

authors： Kim SY,Nishioka M,Hayashi S,Honda H,Kobayashi T,Taya M

更新日期：2005-05-01 00:00:00

abstract：:NAD and its reduced form NADH function as essential redox cofactors and have major roles in determining cellular metabolic features. NAD can be synthesized through the deamidated and amidated pathways, for which the key reaction involves adenylylation of nicotinic acid mononucleotide (NaMN) and nicotinamide mononucleo...

journal_title：Applied and environmental microbiology

authors： Wang X,Zhou YJ,Wang L,Liu W,Liu Y,Peng C,Zhao ZK

更新日期：2017-06-16 00:00:00

abstract：UNLABELLED:Clostridium perfringens type A is a common source of foodborne illness (FBI) in humans. Vegetative cells sporulate in the small intestinal tract and produce the major pathogenic factor C. perfringens enterotoxin. Although sporulation plays a critical role in the pathogenesis of FBI, the mechanisms inducing s...

journal_title：Applied and environmental microbiology

authors： Yasugi M,Okuzaki D,Kuwana R,Takamatsu H,Fujita M,Sarker MR,Miyake M

更新日期：2016-05-02 00:00:00

abstract：:Leaf-cutter ants use plant matter to culture the obligate mutualistic basidiomycete Leucoagaricus gongylophorus. This fungus mediates ant nutrition on plant resources. Furthermore, other microbes living in the fungus garden might also contribute to plant digestion. The fungus garden comprises a young sector with recen...

journal_title：Applied and environmental microbiology

authors： Somera AF,Lima AM,Dos Santos-Neto ÁJ,Lanças FM,Bacci M Jr

更新日期：2015-07-01 00:00:00

abstract：:2-Methyl-6-ethylaniline (MEA) is the main microbial degradation intermediate of the chloroacetanilide herbicides acetochlor and metolachlor. Sphingobium sp. strain MEA3-1 can utilize MEA and various alkyl-substituted aniline and phenol compounds as sole carbon and energy sources for growth. We isolated the mutant stra...

journal_title：Applied and environmental microbiology

authors： Dong W,Chen Q,Hou Y,Li S,Zhuang K,Huang F,Zhou J,Li Z,Wang J,Fu L,Zhang Z,Huang Y,Wang F,Cui Z

更新日期：2015-12-01 00:00:00