Investigation of the Sterol Composition and Azole Resistance in Field Isolates of Septoria tritici.

Abstract:

:We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL2 and S-27 contained isomers of ergosterol and ergosta-5,7-dienol, although in differing concentrations. Under azole treatment, this phytopathogen exhibited a response similar to that of other pathogenic fungi, with a reduction in desmethyl sterols and an accumulation of 14(alpha)-methyl sterols, indicative of inhibition of the P450-mediating sterol 14(alpha)-demethylase. Growth arrest was attributed to the reduction of ergosterol combined with an accumulation of nonutilizable sterols. Strain S-27 exhibited an azole-resistant phenotype which was correlated with decreased cellular content of azole.

journal_name

Appl Environ Microbiol

authors

Joseph-Horne T,Hollomon D,Manning N,Kelly SL

doi

10.1128/AEM.62.1.184-190.1996

keywords:

subject

Has Abstract

pub_date

1996-01-01 00:00:00

pages

184-90

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

62

pub_type

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