Abstract:
:We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL2 and S-27 contained isomers of ergosterol and ergosta-5,7-dienol, although in differing concentrations. Under azole treatment, this phytopathogen exhibited a response similar to that of other pathogenic fungi, with a reduction in desmethyl sterols and an accumulation of 14(alpha)-methyl sterols, indicative of inhibition of the P450-mediating sterol 14(alpha)-demethylase. Growth arrest was attributed to the reduction of ergosterol combined with an accumulation of nonutilizable sterols. Strain S-27 exhibited an azole-resistant phenotype which was correlated with decreased cellular content of azole.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Joseph-Horne T,Hollomon D,Manning N,Kelly SLdoi
10.1128/AEM.62.1.184-190.1996keywords:
subject
Has Abstractpub_date
1996-01-01 00:00:00pages
184-90issue
1eissn
0099-2240issn
1098-5336journal_volume
62pub_type
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