Abstract:
:The effects of water activity (aw, 0.955 to 0.970), pH (4.75 to 5.75), and storage time (up to 60 days) on toxin production by Clostridium botulinum in cooked, vacuum-packed potatoes were studied by using factorial design experiments and most-probable-number methodology. Samples were inoculated with 10(3), 10(4), or 10(5) spores of a mixture of five type A and five proteolytic type B strains, incubated at 25 degrees C, and analyzed for toxin production. Toxin was produced at pH levels of greater than or equal to 4.75 when the aw was greater than or equal to 0.970, pH greater than 5.25 when the aw was 0.965, and pH greater than or equal to 5.75 at an aw of 0.960. No toxin was detected when the aw was 0.955. The probability of toxigenesis was significantly affected (P less than 0.0001) by storage time, aw, pH, and the interactions aw.pH and aw.storage time. The response to a decrease in pH was linear, while the response to a decrease in aw was curvilinear. Using multiple linear regression, equations were derived which could predict the length of time until toxin production and the probability of toxigenesis by a single spore under defined conditions.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Dodds KLdoi
10.1128/AEM.55.3.656-660.1989subject
Has Abstractpub_date
1989-03-01 00:00:00pages
656-60issue
3eissn
0099-2240issn
1098-5336journal_volume
55pub_type
杂志文章abstract::Many cyanobacterial strains are able to grow at a pH range from neutral to pH 10 or 11. Such alkaline conditions favor cyanobacterial growth (e.g., bloom formation), and cyanobacteria must have developed strategies to adjust to changes in CO2 concentration and ion availability. Synechocystis sp. strain PCC 6803 exhibi...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.55.1.254-255.1989
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.60.2.697-704.1994
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更新日期:1986-01-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1993-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1977-06-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2015-05-01 00:00:00
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pub_type: 杂志文章
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更新日期:1978-12-01 00:00:00