Engineering desiccation tolerance in Escherichia coli.

Abstract:

:Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P==O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.

journal_name

Appl Environ Microbiol

authors

Billi D,Wright DJ,Helm RF,Prickett T,Potts M,Crowe JH

doi

10.1128/aem.66.4.1680-1684.2000

keywords:

subject

Has Abstract

pub_date

2000-04-01 00:00:00

pages

1680-4

issue

4

eissn

0099-2240

issn

1098-5336

journal_volume

66

pub_type

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