Abstract:
:Recombinant sucrose-6-phosphate synthase (SpsA) was synthesized in Escherichia coli BL21DE3 by using the spsA gene of the cyanobacterium Synechocystis sp. strain PCC 6803. Transformants exhibited a 10,000-fold increase in survival compared to wild-type cells following either freeze-drying, air drying, or desiccation over phosphorus pentoxide. The phase transition temperatures and vibration frequencies (P==O stretch) in phospholipids suggested that sucrose maintained membrane fluidity during cell dehydration.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Billi D,Wright DJ,Helm RF,Prickett T,Potts M,Crowe JHdoi
10.1128/aem.66.4.1680-1684.2000keywords:
subject
Has Abstractpub_date
2000-04-01 00:00:00pages
1680-4issue
4eissn
0099-2240issn
1098-5336journal_volume
66pub_type
杂志文章abstract::The effects of plasterboard composition on the growth and sporulation of Stachybotrys chartarum as well as on the inflammatory potential of the spores were studied. S. chartarum was grown on 13 modified plasterboards under saturated humidity conditions. The biomass was estimated by measuring the ergosterol content of ...
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