Abstract:
:Swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. This study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions at a subtropical, recreational marine beach in south Florida impacted by non-point sources of pollution. Twelve water and eight sand samples were collected during four sampling events at high or low tide under elevated or reduced solar insolation conditions. The analyses performed included analyses of fecal indicator bacteria (FIB) (fecal coliforms, Escherichia coli, enterococci, and Clostridium perfringens), human-associated microbial source tracking (MST) markers (human polyomaviruses [HPyVs] and Enterococcus faecium esp gene), and pathogens (Vibrio vulnificus, Staphylococcus aureus, enterovirus, norovirus, hepatitis A virus, Cryptosporidium spp., and Giardia spp.). The enterococcus concentrations in water and sand determined by quantitative PCR were greater than the concentrations determined by membrane filtration measurement. The FIB concentrations in water were below the recreational water quality standards for three of the four sampling events, when pathogens and MST markers were also generally undetectable. The FIB levels exceeded regulatory guidelines during one event, and this was accompanied by detection of HPyVs and pathogens, including detection of the autochthonous bacterium V. vulnificus in sand and water, detection of the allochthonous protozoans Giardia spp. in water, and detection of Cryptosporidium spp. in sand samples. The elevated microbial levels were detected at high tide and under low-solar-insolation conditions. Additional sampling should be conducted to further explore the relationships between tidal and solar insolation conditions and between indicator microbes and pathogens in subtropical recreational marine waters impacted by non-point source pollution.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Abdelzaher AM,Wright ME,Ortega C,Solo-Gabriele HM,Miller G,Elmir S,Newman X,Shih P,Bonilla JA,Bonilla TD,Palmer CJ,Scott T,Lukasik J,Harwood VJ,McQuaig S,Sinigalliano C,Gidley M,Plano LR,Zhu X,Wang JD,Fleming LEdoi
10.1128/AEM.02127-09subject
Has Abstractpub_date
2010-02-01 00:00:00pages
724-32issue
3eissn
0099-2240issn
1098-5336pii
AEM.02127-09journal_volume
76pub_type
杂志文章abstract::Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by us...
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doi:10.1128/AEM.37.4.766-773.1979
更新日期:1979-04-01 00:00:00
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更新日期:1980-08-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.57.1.15-18.1991
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1991-11-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.42.6.1074-1084.1981
更新日期:1981-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2019-01-09 00:00:00