Abstract:
:Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reduction of cell density was observed in either cell suspension. It is believed that this is due to the fact that most of the bacterial cells inactivated by microwave radiation remained unlysed. Scanning electron microscopy of the microwave-heated cells revealed severe damage on the surface of most E. coli cells, yet there was no significant change observed in the B. subtilis cells. Microwave-injured E. coli cells were easily lysed in the presence of sodium dodecyl sulfate (SDS), yet B. subtilis cells were resistant to SDS.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Woo IS,Rhee IK,Park HDdoi
10.1128/aem.66.5.2243-2247.2000keywords:
subject
Has Abstractpub_date
2000-05-01 00:00:00pages
2243-7issue
5eissn
0099-2240issn
1098-5336journal_volume
66pub_type
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