Differential damage in bacterial cells by microwave radiation on the basis of cell wall structure.

Abstract:

:Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reduction of cell density was observed in either cell suspension. It is believed that this is due to the fact that most of the bacterial cells inactivated by microwave radiation remained unlysed. Scanning electron microscopy of the microwave-heated cells revealed severe damage on the surface of most E. coli cells, yet there was no significant change observed in the B. subtilis cells. Microwave-injured E. coli cells were easily lysed in the presence of sodium dodecyl sulfate (SDS), yet B. subtilis cells were resistant to SDS.

journal_name

Appl Environ Microbiol

authors

Woo IS,Rhee IK,Park HD

doi

10.1128/aem.66.5.2243-2247.2000

keywords:

subject

Has Abstract

pub_date

2000-05-01 00:00:00

pages

2243-7

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

66

pub_type

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