Effect of solar radiation and predacious microorganisms on survival of fecal and other bacteria.

abstract：:Many fungal pathogens have short generation times, large population sizes, and mixed reproductive systems, providing high potential to adapt to heterogeneous environments of agroecosystems. Such adaptation complicates disease management and threatens food production. A better understanding of pathogen population biolo...

journal_title：Applied and environmental microbiology

authors： Kozhar O,Larsen MM,Grünwald NJ,Peever TL

更新日期：2020-04-17 00:00:00

abstract：:Two PCR primer pairs, based on the virD2 and ipt genes, detected a wide variety of pathogenic Agrobacterium strains. The endonuclease domain of VirD2 protein, which cleaves transferred DNA (T-DNA) border sequences, is highly conserved; primer oligonucleotides specific for the endonuclease portion of virD2 detected all...

journal_title：Applied and environmental microbiology

authors： Haas JH,Moore LW,Ream W,Manulis S

更新日期：1995-08-01 00:00:00

abstract：:Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

journal_title：Applied and environmental microbiology

authors： Fierer N,Jackson JA,Vilgalys R,Jackson RB

更新日期：2005-07-01 00:00:00

abstract：:Pyruvate carboxylase (PYC) is an ecologically, medically, and industrially important enzyme. It is widespread in all three domains of life, the archaea, bacteria, and eukarya. PYC catalyzes ATP-dependent carboxylation of pyruvate to oxaloacetate. Detailed structure-function studies of this enzyme have been hampered du...

journal_title：Applied and environmental microbiology

authors： Lai H,Kraszewski JL,Purwantini E,Mukhopadhyay B

更新日期：2006-12-01 00:00:00

abstract：:Biofilm-associated bacteria are less sensitive to antibiotics than free-living (planktonic) cells. Furthermore, with variations in the concentration of antibiotics throughout a biofilm, microbial cells are often exposed to levels below inhibitory concentrations and may develop resistance. This, as well as the irrespon...

journal_title：Applied and environmental microbiology

authors： Algburi A,Comito N,Kashtanov D,Dicks LMT,Chikindas ML

更新日期：2017-01-17 00:00:00

abstract：:A total of 286 isolates of Pseudomonas maltophilia was collected from sheep exhibiting brown or yellowish fleece rot and from fly-strike lesions. Enzyme activities for 10 of the isolates were examined by plate tests and with the API ZYM system and compared with the enzymatic profile of a human type strain of P. maltop...

journal_title：Applied and environmental microbiology

authors： Macdiarmid JA,Burrell DH

更新日期：1986-02-01 00:00:00

abstract：:The kinetics of denitrification and the causes of nitrite and nitrous oxide accumulation were examined in resting cell suspensions of three denitrifiers. An Alcaligenes species and a Pseudomonas fluorescens isolate characteristically accumulated nitrite when reducing nitrate; a Flavobacterium isolate did not. Nitrate ...

journal_title：Applied and environmental microbiology

authors： Betlach MR,Tiedje JM

更新日期：1981-12-01 00:00:00

abstract：:Molecular tools that can provide an estimate of the in situ growth rate of Geobacter species could improve understanding of dissimilatory metal reduction in a diversity of environments. Whole-genome microarray analyses of a subsurface isolate of Geobacter uraniireducens, grown under a variety of conditions, identified...

journal_title：Applied and environmental microbiology

authors： Holmes DE,Giloteaux L,Barlett M,Chavan MA,Smith JA,Williams KH,Wilkins M,Long P,Lovley DR

更新日期：2013-03-01 00:00:00

abstract：:The hydroaromatic acids shikimate and quinate, which may be available as carbon sources in the soil, supported production of only low levels of acidic exopolysaccharide by Bradyrhizobium japonicum. Exopolysaccharide production (micrograms per 10 cells) was 4.9 on quinate and 4.5 on shikimate; in comparison, it was 128...

journal_title：Applied and environmental microbiology

authors： Tully RE

更新日期：1988-06-01 00:00:00

abstract：:Veratryl alcohol, added as a supplement to cultures of Phanerochaete chrysosporium, enhanced ligninase activity through protection of the ligninase against inactivation by hydrogen peroxide produced by this fungus in cultures. In the presence of veratryl alcohol, the loss of ligninase activity observed in non-protein-...

journal_title：Applied and environmental microbiology

authors： Tonon F,Odier E

更新日期：1988-02-01 00:00:00

abstract：:A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

journal_title：Applied and environmental microbiology

authors： Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

更新日期：1976-09-01 00:00:00

abstract：:A membrane filter technique has been developed for the enumeration of bifidobacteria in natural aquatic environments. The technique is quantitative, selective, and differential. The medium (YN-6) contains: yeast extract, 2.0 g; agar, 1.5 g; polypeptone peptone, 1.0 g; vitamin-free Casamino Acids, 0.8 g; sodium chlorid...

journal_title：Applied and environmental microbiology

authors： Resnick IG,Levin MA

更新日期：1981-09-01 00:00:00

abstract：:The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the...

journal_title：Applied and environmental microbiology

authors： Dehority BA,Tirabasso PA

更新日期：1989-12-01 00:00:00

abstract：:pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Salyers AA

更新日期：1985-10-01 00:00:00

abstract：:Lignocellulose digestion by wood-feeding termites depends on the mutualistic interaction of unusual, flagellate protists located in their hindgut. Most of the flagellates harbor numerous prokaryotic endosymbionts of so-far-unknown identity and function. Using a full-cycle molecular approach, we show here that the endo...

journal_title：Applied and environmental microbiology

authors： Stingl U,Radek R,Yang H,Brune A

更新日期：2005-03-01 00:00:00

abstract：:The sensitivity of different microorganisms, considered as typical representatives of the microflora of soil and water, was established to evaluate the influence of the nonmedical use of antimicrobial agents on bacterial ecology. Only seven strains, six chemoorganotrophs and one chemolithotroph, could be considered as...

journal_title：Applied and environmental microbiology

authors： Van Dijck P,van de Voorde H

更新日期：1976-03-01 00:00:00

abstract：:A unique, coleopteran-active protein, termed eCry3.1Ab, was generated following variable-region exchange of a Bacillus thuringiensis lepidopteran-active protein, Cry1Ab, with a Cry3A region. Our results support the hypothesis that this variable-region exchange is responsible for imparting strong bioactivity against th...

journal_title：Applied and environmental microbiology

authors： Walters FS,deFontes CM,Hart H,Warren GW,Chen JS

更新日期：2010-05-01 00:00:00

abstract：:Four soil temperature and moisture treatment regimens were imposed on Florunner peanuts 94 days after planting in experimental plots in 1980. At harvest (145 days after planting), the incidence of the Aspergillus flavus group and the aflatoxin concentration were greatest in damaged kernels. Extensive colonization of s...

journal_title：Applied and environmental microbiology

authors： Hill RA,Blankenship PD,Cole RJ,Sanders TH

更新日期：1983-02-01 00:00:00

abstract：:rRNA internal transcribed spacer phylogeny showed that Chesapeake Bay is populated with diverse Synechococcus strains, including members of the poorly studied marine cluster B. Marine cluster B prevailed in the upper bay, while marine cluster A was common in the lower bay. Interestingly, marine cluster B Synechococcus...

journal_title：Applied and environmental microbiology

authors： Chen F,Wang K,Kan J,Suzuki MT,Wommack KE

更新日期：2006-03-01 00:00:00

abstract：:The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2001-03-01 00:00:00

abstract：:Iron (Fe) is one of the most important micronutrients for most life forms on earth. While abundant in soil, Fe bioavailability in oxic soil is very low. Under environmental conditions, bacteria need to acquire sufficient Fe to sustain growth while limiting the energy cost of siderophore synthesis. Biofilm formation mi...

journal_title：Applied and environmental microbiology

authors： Rizzi A,Leroux J,Charron-Lamoureux V,Roy S,Beauregard PB,Bellenger JP

更新日期：2020-10-28 00:00:00

abstract：:Smectite clay minerals are abundant in soils and sediments worldwide and are typically rich in Fe. While recent investigations have shown that the structural Fe(III) bound in clay minerals is reduced by microorganisms, previous studies have not tested growth with clay minerals as the sole electron acceptor. Here we ha...

journal_title：Applied and environmental microbiology

authors： Kostka JE,Dalton DD,Skelton H,Dollhopf S,Stucki JW

更新日期：2002-12-01 00:00:00

abstract：:The effects of polychlorinated biphenyl (PCB) and phenanthrene stress on glucose uptake by natural microbial populations were examined by the heterotrophic potential technique. Temporal and spatial distributions in glucose uptake velocities were examined for natural samples as well as PCB- and phenanthrene-stressed sa...

journal_title：Applied and environmental microbiology

authors： Sayler GS,Lund LC,Shiaris MP,Sherrill TW,Perkins RE

更新日期：1979-05-01 00:00:00

abstract：:The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

journal_title：Applied and environmental microbiology

authors： La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

更新日期：2001-12-01 00:00:00

abstract：:We developed a system to monitor the transfer of heterologous DNA from a genetically manipulated strain of Saccharomyces cerevisiae to Escherichia coli. This system is based on a yeast strain that carries multiple integrated copies of a pUC-derived plasmid. The bacterial sequences are maintained in the yeast genome by...

journal_title：Applied and environmental microbiology

authors： Adam AC,González-Blasco G,Rubio-Texeira M,Polaina J

更新日期：1999-12-01 00:00:00

abstract：:Kidneys showing renal changes characteristic for mycotoxic porcine nephropathy were collected during the period 1 April 1982 to 31 March 1983 from 225,000 swine processed in a large slaughterhouse in the district of Poznań, Poland. Of 113 kidneys suspected of mycotoxic porcine nephropathy, 27 exhibited ochratoxin A le...

journal_title：Applied and environmental microbiology

authors： Goliński P,Hult K,Grabarkiewicz-Szczesna J,Chełkowski J,Kneblewski P,Szebiotko K

更新日期：1984-06-01 00:00:00

abstract：:The antimicrobial activities of sucrose monolaurate and a novel ester, lactose monolaurate (LML), were tested. Gram-positive bacteria were more susceptible than Gram-negative bacteria to both esters. The minimal bactericidal concentrations of LML were 5 to 9.5 mM for Listeria monocytogenes isolates and 0.2 to 2 mM for...

journal_title：Applied and environmental microbiology

authors： Wagh A,Shen S,Shen FA,Miller CD,Walsh MK

更新日期：2012-05-01 00:00:00

abstract：:A diesel fuel-contaminated aquifer was bioremediated in situ by the injection of oxidants (O2 and NO3-) and nutrients in order to stimulate microbial activity. After 3.5 years of remediation, an aquifer sample was excavated and the material was used (i) to isolate bacterial strains able to grow on selected hydrocarbon...

journal_title：Applied and environmental microbiology

authors： Hess A,Zarda B,Hahn D,Häner A,Stax D,Höhener P,Zeyer J

更新日期：1997-06-01 00:00:00

abstract：:A computer program was developed to identify anaerobic bacteria by using simultaneous pattern recognition via a Bayesian probabilistic model. The system is intended for use as a rapid, precise, and reproducible aid in the identification of unknown isolates. The program operates on a data base of 28 genera comprising 2...

journal_title：Applied and environmental microbiology

authors： Kelley RW,Kellogg ST

更新日期：1978-03-01 00:00:00

abstract：:A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...

journal_title：Applied and environmental microbiology

authors： Morotomi M,Ohno T,Mutai M

更新日期：1988-05-01 00:00:00