Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.

abstract：:A tremorgenic mycotoxin was isolated from Penicillium paraherquei Abe ex G. Smith and identified as verruculogen. It was produced at the rate of approximately 1 mg/g of the dried fungal mycelium cultured on peptone-enriched Czapek-Dox medium at 28 degrees C. ...

journal_title：Applied and environmental microbiology

authors： Yoshizawa T,Morooka N,Sawada Y,Udagawa SI

更新日期：1976-09-01 00:00:00

abstract：:An assembly consisting of Styrofoam cups with membranes of varying porosities was developed to study microorganism-root interactions. The assembly permitted uniform distribution of a bacterium in soil and was simple, easy to use, and disposable. In tests with the bacterium Pseudomonas solanacearum, little difference i...

journal_title：Applied and environmental microbiology

authors： Hartel PG,Billingsley JW,Williamson JW

更新日期：1989-05-01 00:00:00

abstract：:An in-depth study of the continuous cell line designated BGM is described herein, and recommendations are made for standardizing cell culture and viral assay procedures. Based on data gathered from a survey of 58 laboratories using this cell line, a research plan was developed that included the study of growth media, ...

journal_title：Applied and environmental microbiology

authors： Dahling DR,Wright BA

更新日期：1986-04-01 00:00:00

abstract：:Vibrio parahaemolyticus and Vibrio vulnificus are naturally occurring estuarine bacteria and are the leading causes of seafood-associated infections and mortality in the United States. Though multiple-antibiotic-resistant V. parahaemolyticus and V. vulnificus strains have been reported, resistance patterns in vibrios ...

journal_title：Applied and environmental microbiology

authors： Elmahdi S,Parveen S,Ossai S,DaSilva LV,Jahncke M,Bowers J,Jacobs J

更新日期：2018-01-17 00:00:00

abstract：:To produce recombinant hemoglobin in Escherichia coli, sufficient intracellular heme must be present, or the protein folds improperly and is degraded. In this study, coexpression of human hemoglobin genes and Plesiomonas shigelloides heme transport genes enhanced recombinant hemoglobin production in E. coli BL21(DE3) ...

journal_title：Applied and environmental microbiology

authors： Villarreal DM,Phillips CL,Kelley AM,Villarreal S,Villaloboz A,Hernandez P,Olson JS,Henderson DP

更新日期：2008-09-01 00:00:00

abstract：:Expression of genes encoding polyamine transporters from plasmids and polyamine supplements increased furfural tolerance (growth and ethanol production) in ethanologenic Escherichia coli LY180 (in AM1 mineral salts medium containing xylose). This represents a new approach to increase furfural tolerance and may be usef...

journal_title：Applied and environmental microbiology

authors： Geddes RD,Wang X,Yomano LP,Miller EN,Zheng H,Shanmugam KT,Ingram LO

更新日期：2014-10-01 00:00:00

abstract：:Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), ...

journal_title：Applied and environmental microbiology

authors： Seyer K,Lessard M,Piette G,Lacroix M,Saucier L

更新日期：2003-06-01 00:00:00

abstract：:Phomenoic acid and phomenolactone, previously isolated from the fungus Phoma lingam (Tode) Desm., have shown moderate antifungal and antibacterial properties in vitro. To rationalize the production of phomenoic acid, a kinetic study of its biosynthesis in the mycelium was performed. Phomenoic acid and phomenolactone a...

journal_title：Applied and environmental microbiology

authors： Topgi RS,Devys M,Bousquet JF,Kollmann A,Barbier M

更新日期：1987-05-01 00:00:00

abstract：UNLABELLED:The mycosporine-like amino acids (MAAs) are a group of small molecules with a diverse ecological distribution among microorganisms. MAAs have a range of physiological functions, including protection against UV radiation, making them important from a biotechnological perspective. In the present study, we iden...

journal_title：Applied and environmental microbiology

authors： D'Agostino PM,Javalkote VS,Mazmouz R,Pickford R,Puranik PR,Neilan BA

更新日期：2016-09-16 00:00:00

abstract：:Laboratory studies of methane formation in peat samples from an acid subarctic mire in Sweden indicated the presence of a low-temperature-adapted methanogenic flora. Enrichment culture studies with ethanol, acetate, hydrogen, or a combination of these as substrate for methane formation provided evidence for the existe...

journal_title：Applied and environmental microbiology

authors： Svensson BH

更新日期：1984-08-01 00:00:00

abstract：:Three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. Four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. In situ microbial community structures were monitored by phosph...

journal_title：Applied and environmental microbiology

authors： MacNaughton SJ,Stephen JR,Venosa AD,Davis GA,Chang YJ,White DC

更新日期：1999-08-01 00:00:00

abstract：:Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

journal_title：Applied and environmental microbiology

authors： Baer SH,Bryant DL,Blaschek HP

更新日期：1989-10-01 00:00:00

abstract：:A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiologi...

journal_title：Applied and environmental microbiology

authors： Payne MS,Hall MR,Sly L,Bourne DG

更新日期：2007-03-01 00:00:00

abstract：:Patulin production by Penicillium griseofulvum was monitored with Sep-Pak cartridges and high-pressure liquid chromatography. Determination and quantification of this metabolite proved to be very simple, and our method saved time and a large amount of organic solvents. ...

journal_title：Applied and environmental microbiology

authors： Torres M,Sanchis V,Riba M,Canela R

更新日期：1986-01-01 00:00:00

abstract：:To determine rates of microbial transformation of polycyclic aromatic hydrocarbons (PAH) in freshwater sediments, C-labeled PAH were incubated with samples from both pristine and petroleum-contaminated streams. Evolved CO(2) was trapped in KOH, unaltered PAH and polar metabolic intermediate fractions were quantitated ...

journal_title：Applied and environmental microbiology

authors： Herbes SE,Schwall LR

更新日期：1978-02-01 00:00:00

abstract：:A simple, efficient procedure for removing lactic acid and for reducing nonprotein nitrogen and ash in lactic acid whey has been developed. The procedure consists of culturing Candida ingens on the whey. This organism could assimilate >98% of the lactic acid and approximately 40% of the nonprotein nitrogen. Ash reduct...

journal_title：Applied and environmental microbiology

authors： Ruiz LP,Gurnsey JC,Short JL

更新日期：1978-04-01 00:00:00

abstract：:A gram-negative bacterium which hydrolyzed aryl N-methylcarbamate insecticides was isolated from an agricultural soil which quickly degraded these pesticides. This organism, designated strain ER2, grew on carbofuran as a sole source of carbon and nitrogen with a doubling time of 3 h in a mineral salts medium. The arom...

journal_title：Applied and environmental microbiology

authors： Topp E,Hanson RS,Ringelberg DB,White DC,Wheatcroft R

更新日期：1993-10-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:Fifteen Bacillus thuringiensis strains representing 13 serotypes were screened with five oligodeoxyribonucleotide probes specific for certain regions of two published sequences and one unpublished sequence of B. thuringiensis delta-endotoxin genes. Of the 15 cultures, 14 hybridized with at least one probe; the B. thur...

journal_title：Applied and environmental microbiology

authors： Prefontaine G,Fast P,Lau PC,Hefford MA,Hanna Z,Brousseau R

更新日期：1987-12-01 00:00:00

abstract：:The mode of action of purified aminopeptidase N from Lactococcus lactis subsp. cremoris Wg2 on a complex peptide mixture of a tryptic digest from bovine beta-casein was analyzed. The oligopeptides produced in the tryptic digest before and after aminopeptidase N treatment were identified by analysis of the N- and C-ter...

journal_title：Applied and environmental microbiology

authors： Tan PS,van Kessel TA,van de Veerdonk FL,Zuurendonk PF,Bruins AP,Konings WN

更新日期：1993-05-01 00:00:00

abstract：:Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

journal_title：Applied and environmental microbiology

authors： Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

更新日期：2002-02-01 00:00:00

abstract：:The pigmented strain Serratia marcescens ATCC 274 had a higher buffering capacity and a higher membrane H conductance than S. marcescens GP, a spontaneous nonpigmented mutant of ATCC 274. The data suggest that mutations which apparently affect only the synthesis of a secondary metabolite can modify buffering capacity ...

journal_title：Applied and environmental microbiology

authors： Rius N,Solé M,Francia A,Lorén JG

更新日期：1994-06-01 00:00:00

abstract：:Chitin is massively produced by freshwater plankton species as a structural element of their exoskeleton or cell wall. At the same time, chitin does not accumulate in the predominantly anoxic sediments, underlining its importance as carbon and nitrogen sources for sedimentary microorganisms. We studied chitin degradat...

journal_title：Applied and environmental microbiology

authors： Wörner S,Pester M

更新日期：2019-08-29 00:00:00

abstract：:A Vibrio parahaemolyticus strain isolated from the rhizosphere of the ecosystem dominant estuarine grass, Spartina alterniflora, was characterized and shown to carry nifH, the gene encoding the nitrogenase iron protein, and to fix N(2). Nitrogen fixation may contribute substantially to the adaptability, niche breadth,...

journal_title：Applied and environmental microbiology

authors： Criminger JD,Hazen TH,Sobecky PA,Lovell CR

更新日期：2007-09-01 00:00:00

abstract：:To identify genes that are important for class IIa bacteriocin interaction and resistance in Listeria species, transposon Tn917 knockout libraries were constructed for Listeria innocua strain Lin11 and screened for mutants that are resistant to pediocin AcH. A highly resistant mutant (G7) (MIC > 20 microg/ml; 1,000-fo...

journal_title：Applied and environmental microbiology

authors： Xue J,Hunter I,Steinmetz T,Peters A,Ray B,Miller KW

更新日期：2005-03-01 00:00:00

abstract：:We have developed a microtiter plate method for screening a large number of bacterial isolates for the ability to grow on different crystalline polycyclic aromatic hydrocarbons (PAHs). Growth on PAHs cannot easily be determined with standard growth assays because of the very low aqueous solubility and bioavailability ...

journal_title：Applied and environmental microbiology

authors： Johnsen AR,Bendixen K,Karlson U

更新日期：2002-06-01 00:00:00

abstract：:A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

journal_title：Applied and environmental microbiology

authors： Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

更新日期：1987-08-01 00:00:00

abstract：:Few studies, if any, have addressed the adhesion of enterococci to the intestinal mucosa and their interference with the adhesion of pathogens, although more than 60% of probiotic preparations in the market contain strains of enterococci. The objective of this study was to investigate if Enterococcus faecium 18C23 has...

journal_title：Applied and environmental microbiology

authors： Jin LZ,Marquardt RR,Zhao X

更新日期：2000-10-01 00:00:00

abstract：:A defined solid and liquid minimal medium, HTM, which contained methionine and cysteine as the sole amino acids, was developed for Listeria monocytogenes. Complex broth-grown L. monocytogenes had to adapt to HTM by inducing amino acid biosyntheis. HTM is the simplest minimal medium available for growth of L. monocytog...

journal_title：Applied and environmental microbiology

authors： Tsai HN,Hodgson DA

更新日期：2003-11-01 00:00:00

abstract：:Shuttle vectors that replicate stably and express selectable phenotypes in both Thermococcus kodakaraensis and Escherichia coli have been constructed. Plasmid pTN1 from Thermococcus nautilis was ligated to the commercial vector pCR2.1-TOPO, and selectable markers were added so that T. kodakaraensis transformants could...

journal_title：Applied and environmental microbiology

authors： Santangelo TJ,Cubonová L,Reeve JN

更新日期：2008-05-01 00:00:00