Abstract:
:Biotin-labeled DNA probes prepared from whole plasmids (5.5 and 4.8 kilobases) of two lactobacillus strains (Lactobacillus delbrueckii 21 and Lactobacillus reuteri 100-23) were used to detect the homologous bacteria in microtome-cryostat-prepared sections of murine forestomach. The forestomach sections were incubated on nylon filter membranes placed on agar plates and, after lysis of the lactobacillus cells and denaturation of their DNA, were used in hybridization experiments with the strain-specific DNA probes. Hybridization of the probes to membranes containing sections from lactobacillus-free mice did not occur. The probes detected the presence of homologous strains of lactobacilli in sections cut from the forestomach of mice harboring one or both of the strains.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Tannock GWdoi
10.1128/AEM.55.2.461-464.1989subject
Has Abstractpub_date
1989-02-01 00:00:00pages
461-4issue
2eissn
0099-2240issn
1098-5336journal_volume
55pub_type
杂志文章abstract::Aerobiological monitoring was conducted in an experimental room to aid in the development of standardized sampling protocols for airborne microorganisms in the indoor environment. The objectives of this research were to evaluate the relative efficiencies of selected sampling methods for the retrieval of airborne funga...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.59.1.219-226.1993
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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doi:10.1128/AEM.40.4.798-802.1980
更新日期:1980-10-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.1.41-46.1996
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doi:10.1128/AEM.43.3.643-647.1982
更新日期:1982-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1982-08-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.1.98-100.1986
更新日期:1986-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2006-04-01 00:00:00
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