Characterization of aquatic bacteria and cloning of genes specifying partial degradation of 2,4-dichlorophenoxyacetic acid.

abstract：:We developed a system to monitor the transfer of heterologous DNA from a genetically manipulated strain of Saccharomyces cerevisiae to Escherichia coli. This system is based on a yeast strain that carries multiple integrated copies of a pUC-derived plasmid. The bacterial sequences are maintained in the yeast genome by...

journal_title：Applied and environmental microbiology

authors： Adam AC,González-Blasco G,Rubio-Texeira M,Polaina J

更新日期：1999-12-01 00:00:00

abstract：:Ten phenols were selected as natural laccase mediators after screening 44 different compounds with a recalcitrant dye (Reactive Black 5) as a substrate. Their performances were evaluated at different mediator/dye ratios and incubation times (up to 6 h) by the use of Pycnoporus cinnabarinus and Trametes villosa laccase...

journal_title：Applied and environmental microbiology

authors： Camarero S,Ibarra D,Martínez MJ,Martínez AT

更新日期：2005-04-01 00:00:00

abstract：:In Europe, Borrelia burgdorferi genospecies causing Lyme borreliosis are mainly transmitted by the tick Ixodes ricinus. Since its discovery, B. burgdorferi has been the subject of many epidemiological studies to determine its prevalence and the distribution of the different genospecies in ticks. In the current study w...

journal_title：Applied and environmental microbiology

authors： Rauter C,Hartung T

更新日期：2005-11-01 00:00:00

abstract：:In two intertidal marshes, the vertical distribution in the sediment and inhibition by ammonium of methane oxidation were investigated by slurry incubation experiments. The two sites differ in their dominant vegetation type, i.e., reed and bulrush, and in their heights above sea level. The reed site was elevated with ...

journal_title：Applied and environmental microbiology

authors： Van Der Nat F,De Brouwer J,Middelburg JJ,Laanbroek HJ

更新日期：1997-12-01 00:00:00

abstract：:Recreational and potable water supplies polluted with human wastewater can pose a direct health risk to humans. Therefore, sensitive detection of human fecal pollution in environmental waters is very important to water quality authorities around the globe. Microbial source tracking (MST) utilizes human fecal markers (...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Sidhu JPS,Smith K,Beale DJ,Gyawali P,Toze S

更新日期：2015-12-18 00:00:00

abstract：:Comparisons of animals bearing and lacking microorganisms can offer valuable insight into the interactions between animal hosts and their resident microbiota. Most hosts are naturally infected, and therefore, these comparisons require specific procedures (e.g., antibiotic treatment or physical exclusion of microorgani...

journal_title：Applied and environmental microbiology

authors： Ridley EV,Wong AC,Douglas AE

更新日期：2013-05-01 00:00:00

abstract：:Bactericidal actions of the silver ion on Escherichia coli as a model microorganism were studied using energy-filtering transmission electron microscopy (EFTEM), two-dimensional electrophoresis (2-DE), and matrix-assisted laser desorption ionization-time-of-flight mass spectrometry (MALDI-TOF MS). EFTEM observations d...

journal_title：Applied and environmental microbiology

authors： Yamanaka M,Hara K,Kudo J

更新日期：2005-11-01 00:00:00

abstract：:Confocal scanning laser microscopy (CSLM) was used to demonstrate the attachment of Escherichia coli O157:H7 transformed with a plasmid encoding for green fluorescent protein (GFP) to the surface and within the internal structures of nonwaxed Red Delicious cv. apples. Apples at 2 or 25 degrees C were inoculated with a...

journal_title：Applied and environmental microbiology

authors： Burnett SL,Chen J,Beuchat LR

更新日期：2000-11-01 00:00:00

abstract：:The geochemical cycling of cobalt (Co) has often been considered to be controlled by the scavenging and oxidation of Co(II) on the surface of manganese [Mn(III,IV)] oxides or manganates. Because Mn(II) oxidation in the environment is often catalyzed by bacteria, we have investigated the ability of Mn(II)-oxidizing bac...

journal_title：Applied and environmental microbiology

authors： Lee Y,Tebo BM

更新日期：1994-08-01 00:00:00

abstract：:Hydraulic flush waste removal systems coupled to solid/liquid separators and circulated treatment lagoons are commonly utilized to manage the large amounts of animal waste produced on high-intensity dairy farms. Although these systems are common, little is known about the microbial populations that inhabit them or how...

journal_title：Applied and environmental microbiology

authors： McGarvey JA,Miller WG,Sanchez S,Stanker L

更新日期：2004-07-01 00:00:00

abstract：:A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

journal_title：Applied and environmental microbiology

authors： Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

更新日期：1998-12-01 00:00:00

abstract：:Algal biofuels represent one of the most promising means of sustainably replacing liquid fuels. However, significant challenges remain before alga-based fuels become competitive with fossil fuels. One of the largest challenges is the ability to harvest the algae in an economical and low-energy manner. In this article,...

journal_title：Applied and environmental microbiology

authors： Powell RJ,Hill RT

更新日期：2013-10-01 00:00:00

abstract：:Efficient production of heterologous proteins with yeasts and other eukaryotic hosts is often hampered by inefficient secretion of the product. Limitation of protein secretion has been attributed to a low folding rate, and a rational solution is the overexpression of proteins supporting folding, like protein disulfide...

journal_title：Applied and environmental microbiology

authors： Gasser B,Sauer M,Maurer M,Stadlmayr G,Mattanovich D

更新日期：2007-10-01 00:00:00

abstract：:There is currently little information on nonphosphorylated sugar epimerases, which are of potential interest for producing rare sugars. We found a gene (the TM0416 gene) encoding a putative d-tagatose-3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima We overexpressed the TM0416 gene ...

journal_title：Applied and environmental microbiology

authors： Shin SM,Cao TP,Choi JM,Kim SB,Lee SJ,Lee SH,Lee DW

更新日期：2017-05-01 00:00:00

abstract：:Analysis of food samples for E. coli O157:H7 using the standard U.S. Food and Drug Administration procedure is frequently complicated by overgrowth of nontarget microorganisms. A new procedure was developed for enrichment of enterohemorrhagic E. coli (EHEC) which utilizes exposure to pH 2.00 for 2 h. This procedure yi...

journal_title：Applied and environmental microbiology

authors： Grant MA

更新日期：2004-02-01 00:00:00

abstract：:The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

journal_title：Applied and environmental microbiology

authors： Kaneko T,Holder-Franklin M,Franklin M

更新日期：1977-04-01 00:00:00

abstract：:The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industr...

journal_title：Applied and environmental microbiology

authors： Ginolhac A,Jarrin C,Gillet B,Robe P,Pujic P,Tuphile K,Bertrand H,Vogel TM,Perrière G,Simonet P,Nalin R

更新日期：2004-09-01 00:00:00

abstract：:We examined the competitive relationship between two isolates of denitrifying bacteria, both of which grow well under aerobic conditions but differ in their ability to grow under denitrifying conditions. The growth and persistence of the two isolates, added to sterile soil or added to soil previously colonized by the ...

journal_title：Applied and environmental microbiology

authors： Murray RE,Parsons LL,Smith MS

更新日期：1992-12-01 00:00:00

abstract：:Deletion of the pcaHG genes, encoding protocatechuate 3,4-dioxygenase in Rhodococcus jostii RHA1, gives a gene deletion strain still able to grow on protocatechuic acid as the sole carbon source, indicating a second degradation pathway for protocatechuic acid. Metabolite analysis of wild-type R. jostii RHA1 grown on m...

journal_title：Applied and environmental microbiology

authors： Spence EM,Scott HT,Dumond L,Calvo-Bado L,di Monaco S,Williamson JJ,Persinoti GF,Squina FM,Bugg TDH

更新日期：2020-09-17 00:00:00

abstract：:The thermal inactivation kinetics of Salmonella enteritidis PT4 between 49 and 60 degrees C were investigated. Using procedures designed to eliminate methodological artifacts, we found that the death kinetics deviated from the accepted model of first-order inactivation. When we used high-density stationary-phase popul...

journal_title：Applied and environmental microbiology

authors： Humpheson L,Adams MR,Anderson WA,Cole MB

更新日期：1998-02-01 00:00:00

abstract：:Bdellovibrio bacteriovorus and Micavibrio aeruginosavorus are highly motile Gram-negative predatory bacteria with the potential of being used as biocontrol agents or living antibiotics. It was suggested previously that sugar-binding proteins play a role in M. aeruginosavorus and B. bacteriovorus host specificity and p...

journal_title：Applied and environmental microbiology

authors： Dashiff A,Keeling TG,Kadouri DE

更新日期：2011-04-01 00:00:00

abstract：:A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...

journal_title：Applied and environmental microbiology

authors： Visser R,Holzapfel WH,Bezuidenhout JJ,Kotzé JM

更新日期：1986-09-01 00:00:00

abstract：:Taxonomic and phylogenetic fingerprinting based on sequence analysis of gene fragments from the large-subunit rRNA (LSU) gene or the internal transcribed spacer (ITS) region is becoming an integral part of fungal classification. The lack of an accurate and robust classification tool trained by a validated sequence dat...

journal_title：Applied and environmental microbiology

authors： Liu KL,Porras-Alfaro A,Kuske CR,Eichorst SA,Xie G

更新日期：2012-03-01 00:00:00

abstract：:The bioconversion of waste paper to single-cell protein at pH <1 by Scytalidium acidophilum is described. Waste paper pretreated with 72% H(2)SO(4) at 4 degrees C was diluted with water to a pH of <0.1 and hydrolyzed. This yielded an adequate sugar-containing substrate for the growth of the fungus. A total of 97% of t...

journal_title：Applied and environmental microbiology

authors： Ivarson KC,Morita H

更新日期：1982-03-01 00:00:00

abstract：:Processes by which fecal bacteria enter overland flow and their transportation state to surface waters are poorly understood, making the effectiveness of measures designed to intercept this pathway, such as vegetated buffer strips, difficult to predict. Freshly made and aged (up to 30 days) cowpats were exposed to sim...

journal_title：Applied and environmental microbiology

authors： Muirhead RW,Collins RP,Bremer PJ

更新日期：2005-06-01 00:00:00

abstract：:Nine immunoglobulin G and nine immunoglobulin M murine monoclonal antibody-producing hybridomas reactive with live Salmonella bacteria were obtained from several fusions of immune spleen cells and Sp2/0 myeloma cells. The antibodies were selected by the magnetic immunoluminescence assay. The monoclonal antibodies were...

journal_title：Applied and environmental microbiology

authors： Torensma R,Visser MJ,Aarsman CJ,Poppelier MJ,van Beurden R,Fluit AC,Verhoef J

更新日期：1992-12-01 00:00:00

abstract：:The deep-sea hydrothermal vent shrimp Rimicaris exoculata largely depends on a dense epibiotic chemoautotrophic bacterial community within its enlarged cephalothoracic chamber. However, our understanding of shrimp-bacterium interactions is limited. In this report, we focused on the deltaproteobacterial epibiont of R. ...

journal_title：Applied and environmental microbiology

authors： Jiang L,Liu X,Dong C,Huang Z,Cambon-Bonavita MA,Alain K,Gu L,Wang S,Shao Z

更新日期：2020-04-01 00:00:00

abstract：:The stability of spheroplasts from the osmotrophic yeast Saccharomyces rouxii was studied in buffered solutions of mannitol and glucose. The plasma membranes from cells grown in high glucose concentrations were more stable to osmotic lysis than were membranes from cells grown in lower glucose concentrations. Mannitol ...

journal_title：Applied and environmental microbiology

authors： Moran JW,Witter LD

更新日期：1980-04-01 00:00:00

abstract：:A genetic transformation system with similarities to those reported for gram-negative bacteria was found to be associated with membrane vesicles of the ruminal cellulolytic genus Ruminococcus. Double-stranded DNA was recovered from the subcellular particulate fraction of all the cellulolytic ruminococci examined. Elec...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Yokoyama MT,Forster RJ,Ouwerkerk D,Bain PA,Mawhinney EL

更新日期：2005-08-01 00:00:00

abstract：:The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

journal_title：Applied and environmental microbiology

authors： Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

更新日期：2005-09-01 00:00:00