Abstract:
:The initial microbial colonization of tooth surfaces is a repeatable and selective process, with certain bacterial species predominating in the nascent biofilm. Characterization of the initial microflora is the first step in understanding interactions among community members that shape ensuing biofilm development. Using molecular methods and a retrievable enamel chip model, we characterized the microbial diversity of early dental biofilms in three subjects. A total of 531 16S rRNA gene sequences were analyzed, and 97 distinct phylotypes were identified. Microbial community composition was shown to be statistically different among subjects. In all subjects, however, 4-h and 8-h communities were dominated by Streptococcus spp. belonging to the Streptococcus oralis/Streptococcus mitis group. Other frequently observed genera (comprising at least 5% of clone sequences in at least one of the six clone libraries) were Actinomyces, Gemella, Granulicatella, Neisseria, Prevotella, Rothia, and Veillonella. Fluorescence in situ hybridization (FISH) confirmed that the proportion of Streptococcus sp. sequences in the clone libraries coincided with the proportion of streptococcus probe-positive organisms on the chip. FISH also revealed that, in the undisturbed plaque, not only Streptococcus spp. but also the rarer Prevotella spp. were usually seen in small multigeneric clusters of cells. This study shows that the initial dental plaque community of each subject is unique in terms of diversity and composition. Repetitive and distinctive community composition within subjects suggests that the spatiotemporal interactions and ecological shifts that accompany biofilm maturation also occur in a subject-dependent manner.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Diaz PI,Chalmers NI,Rickard AH,Kong C,Milburn CL,Palmer RJ Jr,Kolenbrander PEdoi
10.1128/AEM.72.4.2837-2848.2006subject
Has Abstractpub_date
2006-04-01 00:00:00pages
2837-48issue
4eissn
0099-2240issn
1098-5336pii
72/4/2837journal_volume
72pub_type
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pub_type: 杂志文章
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pub_type: 杂志文章
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更新日期:2004-05-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1983-03-01 00:00:00
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journal_title:Applied and environmental microbiology
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