Glycosidases of the rumen anaerobic fungus Neocallimastix frontalis grown on cellulosic substrates.

Abstract:

:The rumen anaerobic fungus Neocallimastix frontalis was grown on cellulosic substrates, and the cellular distribution and types of glycosidases produced by the organism were studied. Fungal cultures were fractionated into extracellular, insoluble (membrane), and intracellular fractions and assayed for glycosidase activity by using Avicel, carboxymethylcellulose, xylan, starch, polygalacturonic acid, and the p-nitrophenyl derivatives of galactose, glucose, and xylose as substrates. Enzymic activity was highest in the extracellular fraction; however, the membrane fraction also displayed appreciable activity. The intracellular fraction was inactive towards all substrates. Polygalacturonic acid was the only substrate not hydrolyzed by the active fractions, indicating that pectinase was absent. The results show that N. frontalis, a common rumen anaerobic fungus, produces enzymes for degrading cellulose and hemicellulose, key components of plant fiber.

journal_name

Appl Environ Microbiol

authors

Pearce PD,Bauchop T

doi

10.1128/AEM.49.5.1265-1269.1985

subject

Has Abstract

pub_date

1985-05-01 00:00:00

pages

1265-9

issue

5

eissn

0099-2240

issn

1098-5336

journal_volume

49

pub_type

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