Abstract:
:Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Strovas TJ,Dragavon JM,Hankins TJ,Callis JB,Burgess LW,Lidstrom MEdoi
10.1128/AEM.72.2.1692-1695.2006keywords:
subject
Has Abstractpub_date
2006-02-01 00:00:00pages
1692-5issue
2eissn
0099-2240issn
1098-5336pii
72/2/1692journal_volume
72pub_type
杂志文章abstract::The enzymatic production of 2,5-furandicarboxylic acid (FDCA) from 5-hydroxymethylfurfural (HMF) has gained interest in recent years, as FDCA is a renewable precursor of poly(ethylene-2,5-furandicarboxylate) (PEF). 5-Hydroxymethylfurfural oxidases (HMFOs) form a flavoenzyme family with genes annotated in a dozen bacte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00842-20
更新日期:2020-08-03 00:00:00
abstract::Cowpea is a legume of great agronomic importance that establishes symbiotic relationships with nitrogen-fixing bacteria. However, little is known about the genetic and symbiotic diversity of these bacteria in distinct ecosystems. Our study evaluated the genetic diversity and symbiotic efficiencies of 119 bacterial str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01303-12
更新日期:2012-09-01 00:00:00
abstract::A new bacterial pathogen of chinook salmon (oncorhynchus tshawytscha) was isolated from fish in Oregon's Rogue River. The bacteria are biochemically and serologically related to strains of Edwardsiella tarda. Initially isolated from chinook salmon, the bacteria were also pathogenic for steelhead and rainbow trout (Sal...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.6.1380-1384.1982
更新日期:1982-06-01 00:00:00
abstract::An investigation of the Hg2+ resistance mechanism of four freshwater and four coastal marine bacteria that did not hybridize with a mer operonic probe was conducted (T. Barkay, C. Liebert, and M. Gillman, Appl. Environ. Microbiol. 55:1196-1202, 1989). Hybridization with a merA probe, the gene encoding the mercuric red...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.6.1695-1701.1990
更新日期:1990-06-01 00:00:00
abstract::A thorough understanding of the microorganisms and pathogens associated with the larval stage of the tropical ornate rock lobster, Panulirus ornatus, is required to overcome disease outbreaks that currently block aquaculture attempts. This study used microscopy in addition to culture and molecularly based microbiologi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02520-06
更新日期:2007-03-01 00:00:00
abstract::Streptococcus mutans 6715-15 and Streptococcus sanguis 10558 were grown together in continuous culture with glucose as the limiting carbon source. The relationship of growth rate to substrate concentration was determined for pure cultures of each organism in continuous and batch cultures. A model based on competition ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.4.1277-1282.1983
更新日期:1983-04-01 00:00:00
abstract::o-Methoxycinnamaldehyde has been isolated and purified from powdered cinnamon. The compound inhibits the growth and toxin production of mycotoxin-producing fungi. The substance completely inhibited the growth of Aspergillus parasiticus and A. flavus at 100 microgram/ml and A. ochraceus and A. versicolor at 200 microgr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.4.577-583.1978
更新日期:1978-10-01 00:00:00
abstract::High numbers (10(7) to 10(10) cells per g [dry weight]) of heterotrophic, gram-negative, rod-shaped, non-sporeforming, aerobic, thermophilic bacteria related to the genus Thermus were isolated from thermogenic composts at temperatures between 65 and 82 degrees C. These bacteria were present in different types of waste...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.5.1723-1727.1996
更新日期:1996-05-01 00:00:00
abstract::Quantitative in situ determination of conjugative gene transfer in defined bacterial biofilms using automated confocal laser scanning microscopy followed by three-dimensional analysis of cellular biovolumes revealed conjugation rates 1,000-fold higher than those determined by classical plating techniques. Conjugation ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.8.3710-3713.1999
更新日期:1999-08-01 00:00:00
abstract::A defined medium for growth of 24 strains of Moraxella (Branhamella) catarrhalis was devised. This medium (medium B4) contains sodium lactate as a partial carbon source, proline as both a partial carbon source and a partial nitrogen source, aspartate as a partial nitrogen source, and the growth factors arginine, glyci...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.3.546-551.1986
更新日期:1986-09-01 00:00:00
abstract::Stachybotrys atra is the etiologic agent of stachybotryotoxicosis, and this fungus and its trichothecene mycotoxins were recently implicated in an outbreak of unexplained illness in homes. S. atra was grown on sterile rice, autoclaved, dried, and then aerosolized by acoustic vibration. The distribution of particles (m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.6.1370-1375.1987
更新日期:1987-06-01 00:00:00
abstract::Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.2.901-908.2003
更新日期:2003-02-01 00:00:00
abstract::Two highly active trifunctional hemicellulases were constructed by linking the catalytic portion of a xylanase with an arabinofuranosidase and a xylosidase, using either flexible peptide linkers or linkers containing a cellulose-binding domain. The multifunctional enzymes retain the parental enzyme properties and exhi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02181-08
更新日期:2009-03-01 00:00:00
abstract::The effects of UV-B radiation on the heterotrophic nanoflagellate Bodo saltans (Kinetoplastida) were examined under controlled conditions with artificial UV sources and also under natural solar radiation in an oligotrophic lake. In both types of experiments, the characteristic elongated cell morphology of this flagell...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.12.4395-4400.1996
更新日期:1996-12-01 00:00:00
abstract::It has been suggested that the human gut microbiota can be divided into enterotypes based on the abundance of specific bacterial groups; however, the biological significance and stability of these enterotypes remain unresolved. Here, we demonstrated that subjects (n = 62) 18 to 65 years old with central obesity and co...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章,随机对照试验
doi:10.1128/AEM.03549-13
更新日期:2014-02-01 00:00:00
abstract::Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.12.2924-2932.1988
更新日期:1988-12-01 00:00:00
abstract::The presence of enteroviruses in oysters and oyster-harvesting waters of the Texas Gulf coast was monitored over a period of 10 months. Viruses were detected in water and oyster samples obtained from areas both open and closed to shellfish harvesting. Viruses were detected periodically in waters that met current bacte...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.37.3.572-581.1979
更新日期:1979-03-01 00:00:00
abstract::The beta-subgroup of the Proteobacteria has been shown to be important in aquatic habitats and was investigated in depth here by molecular 16S rRNA techniques in biofilms of the Elbe River and its polluted tributary, the Spittelwasser River. The bacterial 16S rRNA genes were cloned from each site, screened for beta-pr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.8.4463-4473.2003
更新日期:2003-08-01 00:00:00
abstract::Colonization of apple leaves by the yeastlike fungus Aureobasidium pullulans was followed quantitatively and spatially at a microscale level throughout two growing seasons. Ten field leaves were sampled on 11 dates in 2003 and 15 dates in 2004. Using an A. pullulans-specific fluorescence in situ hybridization probe an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00744-06
更新日期:2006-09-01 00:00:00
abstract::To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02279-13
更新日期:2014-01-01 00:00:00
abstract::The scarcity of fresh water in the Mediterranean region necessitates the search for halotolerant agents of biological control of plant diseases that can be applied in arid-zone agriculture irrigated with saline water. Among 29 Trichoderma strains previously isolated from Mediterranean Psammocinia sp. sponges, the grea...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00541-11
更新日期:2011-08-01 00:00:00
abstract::Two constructs derived from the alpha-amylase gene (amyA) of Lactobacillus amylovorus were expressed in Lactobacillus plantarum, and their expression products were purified, characterized, and compared. These products correspond to the complete (AmyA) and truncated (AmyADelta) forms of alpha-amylase; AmyADelta lacks t...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.8.3350-3356.2000
更新日期:2000-08-01 00:00:00
abstract::Aerobic and anaerobic microorganisms were enumerated in tubercles collected from sections of the water distribution pipeline in the Columbus, Ohio, metropolitan area. Coliform bacteria were not detected in the tubercles examined. Sulfate-reducing bacteria were detected in 80% of the samples. Nitrate-reducing heterotro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.44.3.761-764.1982
更新日期:1982-09-01 00:00:00
abstract::We report here a biochemical study of resistance to azole antifungal agents in a field isolate (S-27) of a fungal phytopathogen. Isolates of Septoria tritici were compared in vitro, and their responses reflected that observed in the field, with S-27 exhibiting resistance relative to RL2. In untreated cultures, both RL...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.1.184-190.1996
更新日期:1996-01-01 00:00:00
abstract::In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran d...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01627-07
更新日期:2008-02-01 00:00:00
abstract::Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.6.1374-1377.1981
更新日期:1981-06-01 00:00:00
abstract::Carbazole 1,9a-dioxygenase (CARDO), a Rieske nonheme iron oxygenase (RO), is a three-component system composed of a terminal oxygenase (Oxy), ferredoxin, and a ferredoxin reductase. Oxy has angular dioxygenation activity against carbazole. Previously, site-directed mutagenesis of the Oxy-encoding gene from Janthinobac...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.04000-13
更新日期:2014-05-01 00:00:00
abstract::Lantibiotics are antimicrobial peptides belonging to the family of ribosomally synthesized and posttranslationally modified peptides (RiPPs) and feature thioether linkages in their structures. In this study, we identified the biosynthetic gene cluster of a cinnamycin analog, named mathermycin, from Marinactinospora th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00926-17
更新日期:2017-07-17 00:00:00
abstract::The Mediterranean fruit fly (Ceratitis capitata) is a cosmopolitan pest of hundreds of species of commercial and wild fruits. It is considered a major economic pest of commercial fruits in the world. Adult Mediterranean fruit flies feed on all sorts of protein sources, including animal excreta, in order to develop egg...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.7.4052-4056.2005
更新日期:2005-07-01 00:00:00
abstract::The methanotrophic bacterium Methylosinus trichosporium OB3b is unusually active in degrading recalcitrant haloalkanes such as trichloroethylene (TCE). The first and rate-limiting step in the degradation of TCE is catalyzed by a soluble methane monooxygenase (sMMO). This enzyme is not expressed when the cells are grow...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.11.3701-3708.1992
更新日期:1992-11-01 00:00:00