Measurement of respiration rates of Methylobacterium extorquens AM1 cultures by use of a phosphorescence-based sensor.

Abstract:

:Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This method was tested with the facultative methylotroph Methylobacterium extorquens AM1 to demonstrate subtle mutant phenotypes.

journal_name

Appl Environ Microbiol

authors

Strovas TJ,Dragavon JM,Hankins TJ,Callis JB,Burgess LW,Lidstrom ME

doi

10.1128/AEM.72.2.1692-1695.2006

keywords:

subject

Has Abstract

pub_date

2006-02-01 00:00:00

pages

1692-5

issue

2

eissn

0099-2240

issn

1098-5336

pii

72/2/1692

journal_volume

72

pub_type

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