Characterization of a novel angular dioxygenase from fluorene-degrading Sphingomonas sp. strain LB126.

abstract：:We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA trans...

journal_title：Applied and environmental microbiology

authors： Muscariello L,Marasco R,De Felice M,Sacco M

更新日期：2001-07-01 00:00:00

abstract：:A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

journal_title：Applied and environmental microbiology

authors： Kennedy MJ,Lawless JG

更新日期：1985-01-01 00:00:00

abstract：:Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that inf...

journal_title：Applied and environmental microbiology

authors： Whistler CA,Stockwell VO,Loper JE

更新日期：2000-07-01 00:00:00

abstract：:Salinity effects on microbial community structure and on potential rates of arsenate reduction, arsenite oxidation, sulfate reduction, denitrification, and methanogenesis were examined in sediment slurries from two California soda lakes. We conducted experiments with Mono Lake and Searles Lake sediments over a wide ra...

journal_title：Applied and environmental microbiology

authors： Kulp TR,Han S,Saltikov CW,Lanoil BD,Zargar K,Oremland RS

更新日期：2007-08-01 00:00:00

abstract：:Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 1...

journal_title：Applied and environmental microbiology

authors： Humrighouse BW,Adcock NJ,Rice EW

更新日期：2011-09-01 00:00:00

abstract：:The diversity and dynamics of a bacterial community extracted from an exploited oil field with high natural soil salinity near Comodoro Rivadavia in Patagonia (Argentina) were investigated. Community shifts during long-term incubation with diesel fuel at four salinities between 0 and 20% NaCl were monitored by single-...

journal_title：Applied and environmental microbiology

authors： Kleinsteuber S,Riis V,Fetzer I,Harms H,Müller S

更新日期：2006-05-01 00:00:00

abstract：:Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

journal_title：Applied and environmental microbiology

authors： Goodwin PH,Kirkpatrick BC,Duniway JM

更新日期：1990-03-01 00:00:00

abstract：:We describe here an improved method for isolating, purifying, and cloning DNA from diverse soil microbiota. Soil microorganisms were extracted from soils and embedded and lysed within an agarose plug. Nucleases that copurified with the metagenomic DNA were removed by incubating plugs with a high-salt and -formamide so...

journal_title：Applied and environmental microbiology

authors： Liles MR,Williamson LL,Rodbumrer J,Torsvik V,Goodman RM,Handelsman J

更新日期：2008-05-01 00:00:00

abstract：:A six-step biochemical key is presented for the identification of all recognized Enterococcus spp. The key consists of 12 tests, but no more than 6 are needed for the most complicated identification. The reliability of the key has been evaluated with collection type strains and clinical and environmental isolates. Thi...

journal_title：Applied and environmental microbiology

authors： Manero A,Blanch AR

更新日期：1999-10-01 00:00:00

abstract：:[This corrects the article on p. 483 in vol. 37.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1979-09-01 00:00:00

abstract：:One fluorescent compound previously named TDP-2 was isolated and purified from a rice culture of Fusarium equiseti (Alaska 2-2). Mass spectral and nuclear magnetic resonance data indicated that it is a C-3'-N-acetyl derivative of fusarochromanone, a newly discovered mycotoxin. Time course studies of synthesis of these...

journal_title：Applied and environmental microbiology

authors： Xie WP,Mirocha CJ,Pawlosky RJ,Wen YC,Xu XG

更新日期：1989-04-01 00:00:00

abstract：:A gene coding for an aminopeptidase (PepC) from Lactococcus lactis subsp. cremoris AM2 was cloned by complementation of an Escherichia coli mutant lacking aminopeptidase activity. The nucleotide sequence was determined. A portion of the predicted amino acid sequence of PepC (436 amino acids) showed strong homology to ...

journal_title：Applied and environmental microbiology

authors： Chapot-Chartier MP,Nardi M,Chopin MC,Chopin A,Gripon JC

更新日期：1993-01-01 00:00:00

abstract：:Vinyl chloride reductases (VC-RDase) are the key enzymes for complete microbial reductive dehalogenation of chloroethenes, including the groundwater pollutants tetrachloroethene and trichloroethene. Analysis of the codon usage of the VC-RDase genes vcrA and bvcA showed that these genes are highly unusual and are chara...

journal_title：Applied and environmental microbiology

authors： McMurdie PJ,Behrens SF,Holmes S,Spormann AM

更新日期：2007-04-01 00:00:00

abstract：:A membrane filter procedure has been developed for the rapid quantitation of C. perfringens in the aquatic environment. Background growth is inhibited by the use of D-cycloserine, polymyxin B sulfate, and incubation at 45 degrees C. Differential characteristics include the fermentation of sucrose, production of acid p...

journal_title：Applied and environmental microbiology

authors： Bisson JW,Cabelli VJ

更新日期：1979-01-01 00:00:00

abstract：:Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxi...

journal_title：Applied and environmental microbiology

authors： Quelas JI,Mesa S,Mongiardini EJ,Jendrossek D,Lodeiro AR

更新日期：2016-06-30 00:00:00

abstract：:Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were ...

journal_title：Applied and environmental microbiology

authors： Donegan K,Fieland V,Fowles N,Ganio L,Seidler R

更新日期：1992-04-01 00:00:00

abstract：:The arginine deiminase system in a variety of streptococci and in Pseudomonas aeruginosa was found to be unusually acid tolerant in that arginolysis occurred at pH values well below the minima for growth and glycolysis. The acid tolerance of the system allowed bacteria to survive potentially lethal acidification throu...

journal_title：Applied and environmental microbiology

authors： Marquis RE,Bender GR,Murray DR,Wong A

更新日期：1987-01-01 00:00:00

abstract：:Stability of the plasmid pKK223-200 in Escherichia coli JM105 was studied for both free and immobilized cells during continuous culture. The relationship between plasmid copy number, xylanase activity, which was coded for by the plasmid, and growth rate and culture conditions involved complex interactions which determ...

journal_title：Applied and environmental microbiology

authors： de Taxis du Poët P,Arcand Y,Bernier R Jr,Barbotin JN,Thomas D

更新日期：1987-07-01 00:00:00

abstract：:Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early e...

journal_title：Applied and environmental microbiology

authors： Calzada J,Zamarro MT,Alcón A,Santos VE,Díaz E,García JL,Garcia-Ochoa F

更新日期：2009-02-01 00:00:00

abstract：:Brevibacterium linens M18, isolated from red smear cheese, produces a substance that inhibits the growth of Listeria spp. and several coryneform and other gram-positive bacteria. No gram-negative bacteria were inhibited. The substance is heat labile, sensitive to proteolytic enzymes, and stable between pH 3 and 12. Hi...

journal_title：Applied and environmental microbiology

authors： Valdés-Stauber N,Scherer S

更新日期：1994-10-01 00:00:00

abstract：:Direct electron microscopy of bacteriophages adsorbed to a carbon film without prior enrichment by specific host strains or concentration by physical or chemical methods was used to study the morphological diversity of natural bacteriophage assemblages in a North German lake. All samples contained a mixture of morphol...

journal_title：Applied and environmental microbiology

authors： Demuth J,Neve H,Witzel KP

更新日期：1993-10-01 00:00:00

abstract：:A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...

journal_title：Applied and environmental microbiology

authors： Paillard D,Dubois V,Duran R,Nathier F,Guittet C,Caumette P,Quentin C

更新日期：2003-11-01 00:00:00

abstract：:The objective of this study was to explore the long-term effects of different organic and inorganic fertilizers on activity and composition of the denitrifying and total bacterial communities in arable soil. Soil from the following six treatments was analyzed in an experimental field site established in 1956: cattle m...

journal_title：Applied and environmental microbiology

authors： Enwall K,Philippot L,Hallin S

更新日期：2005-12-01 00:00:00

abstract：:A device for sampling at depths of up to 6,000 m is described in which 3 liters of seawater is concentrated over a Nucleopore filter to about 13 ml and retrieved under in situ pressure and temperature. Subsamples can be withdrawn into transfer units that are equipped with individual gas accumulators for preventing los...

journal_title：Applied and environmental microbiology

authors： Jannasch HW,Wirsen CO

更新日期：1977-03-01 00:00:00

abstract：:pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Salyers AA

更新日期：1985-10-01 00:00:00

abstract：:Epimerization of sugar nucleotides is central to the structural diversification of monosaccharide building blocks for cellular biosynthesis. Epimerase applicability to carbohydrate synthesis can be limited, however, by the high degree of substrate specificity exhibited by most sugar nucleotide epimerases. Here, we dis...

journal_title：Applied and environmental microbiology

authors： Rapp C,van Overtveldt S,Beerens K,Weber H,Desmet T,Nidetzky B

更新日期：2020-12-04 00:00:00

abstract：:Bacterial strains were isolated on the basis of their ability to proliferate in a minimal medium containing one of a series of lignin-related compounds as the sole carbon and energy source. These included the aromatic monomers guaiacol, vanillic and coumaric acids, a dimer and a trimer possessing the arylglycerol-beta...

journal_title：Applied and environmental microbiology

authors： Gonzalez B,Merino A,Almeida M,Vicña R

更新日期：1986-12-01 00:00:00

abstract：:A total of 530 strains of Staphylococcus aureus were isolated from the defeathering machinery of a chicken processing plant and from neck skin samples of carcasses at different stages of processing in two visits 4 weeks apart. Eleven different plasmid profiles were detected in the isolates, eight being common to both ...

journal_title：Applied and environmental microbiology

authors： Dodd CE,Chaffey BJ,Waites WM

更新日期：1988-06-01 00:00:00

abstract：:We report an ex vivo method, which uses pieces of human skin excised during routine plastic surgery, for testing survival of hazardous pathogens. Using this procedure, we compared the survival of human herpesvirus 2 on human skin and on metal disks. At the physiological skin temperature of 32 degrees C, the half-life ...

journal_title：Applied and environmental microbiology

authors： Graham ML,Springthorpe VS,Sattar SA

更新日期：1996-11-01 00:00:00

abstract：:Glycogen debranching enzyme (GDE) has 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities in the single polypeptide chain. We analyzed the detailed action profile of GDE from Saccharomyces cerevisiae on amylose and tested whether GDE catalyzes cyclization of amylose. GDE treatment resulted in a rapid reduc...

journal_title：Applied and environmental microbiology

authors： Yanase M,Takata H,Takaha T,Kuriki T,Smith SM,Okada S

更新日期：2002-09-01 00:00:00