Ex vivo protocol for testing virus survival on human skin: experiments with herpesvirus 2.

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:Nutritional and physical factors which influence the germination of akinetes of Nodularia spumigena (Cyanobacteriaceae) were examined. Low concentrations of phosphorus (<0.9 muM) were required for germination. Nitrate had no effect, but ammonia, at concentrations of >45 muM, inhibited germination. Salinities of >20 pe...

journal_title：Applied and environmental microbiology

authors： Huber AL

更新日期：1985-01-01 00:00:00

abstract：:The contributions of various components of soil microflora and microfauna to rhizosphere phosphatase activity were determined with hydroponic cultures. Three treatments were employed: (i) plants alone (Bouteloua gracilis (H.B.K.) Lag. ex Steud.) (ii) plants plus bacteria (Pseudomonas sp.), and (iii) plants plus bacter...

journal_title：Applied and environmental microbiology

authors： Gould WD,Coleman DC,Rubink AJ

更新日期：1979-05-01 00:00:00

abstract：:The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the major ribotypes. DGGE profiles of the...

journal_title：Applied and environmental microbiology

authors： Favier CF,Vaughan EE,De Vos WM,Akkermans AD

更新日期：2002-01-01 00:00:00

abstract：:In two previous studies dealing with lactic acid bacteria (LAB) from modified-atmosphere-packaged (MAP) broiler products and a broiler processing plant, several isolates remained unidentified. According to 16S rRNA gene sequence analysis, 36 isolates were assigned to the genus Enterococcus. Numerical analysis of combi...

journal_title：Applied and environmental microbiology

authors： Rahkila R,Johansson P,Säde E,Björkroth J

更新日期：2011-02-01 00:00:00

abstract：:Current agricultural practices involve inclusion of antimicrobials in animal feed and result in manure containing antimicrobials and antimicrobial-resistant microorganisms. This work evaluated the effects of land application of swine manure on the levels of tetracycline, macrolide, and lincosamide antimicrobials and o...

journal_title：Applied and environmental microbiology

authors： Zhou Z,Raskin L,Zilles JL

更新日期：2010-04-01 00:00:00

abstract：:In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran d...

journal_title：Applied and environmental microbiology

authors： Schuler L,Ní Chadhain SM,Jouanneau Y,Meyer C,Zylstra GJ,Hols P,Agathos SN

更新日期：2008-02-01 00:00:00

abstract：:We previously reported the isolation of novel methanogens by using a new cultivation method, referred to as the coculture method. Here, we extended our coculture method to various anaerobic environmental samples. As a result, we successfully cultivated some uncharacterized methanogens in coculture enrichments and even...

journal_title：Applied and environmental microbiology

authors： Sakai S,Imachi H,Sekiguchi Y,Tseng IC,Ohashi A,Harada H,Kamagata Y

更新日期：2009-07-01 00:00:00

abstract：:Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subg...

journal_title：Applied and environmental microbiology

authors： Morris RM,Rappé MS,Urbach E,Connon SA,Giovannoni SJ

更新日期：2004-05-01 00:00:00

abstract：:The inhibitory effects of sterigmatocystin, O-methylsterigmatocystin, and 5,6-dimethoxysterigmatocystin on the ATP synthesis system in mitochondria were compared with that of aflatoxin B1, which disturbs the respiratory chain in mitochondria. Sterigmatocystin and 5,6-dimethoxysterigmatocystin were found to uncouple th...

journal_title：Applied and environmental microbiology

authors： Kawai K,Nakamaru T,Nozawa Y,Maebayashi Y,Yamazaki M,Natori S

更新日期：1984-11-01 00:00:00

abstract：:A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

journal_title：Applied and environmental microbiology

authors： Chen H,Ponniah G,Salonen N,Blum P

更新日期：2004-08-01 00:00:00

abstract：:Coumarin concentrations that inhibited growth of Pythium sp. rapidly decreased the rate of incorporation of [14C]glucose into the mycelium. Coumarin also reduced drastically the carbohydrate and protein content of the cytoplasm and, to a lesser extent, the amino acid content and cell wall fraction. Enzymes related to ...

journal_title：Applied and environmental microbiology

authors： Neto FC,Dietrich SM

更新日期：1977-09-01 00:00:00

abstract：:Cereulide and valinomycin are highly similar cyclic dodecadepsipeptides with potassium ionophoric properties. Cereulide, produced by members of the Bacillus cereus group, is known mostly as emetic toxin, and no ecological function has been assigned. A comparative analysis of the antimicrobial activity of valinomycin p...

journal_title：Applied and environmental microbiology

authors： Tempelaars MH,Rodrigues S,Abee T

更新日期：2011-04-01 00:00:00

abstract：:Few studies, if any, have addressed the adhesion of enterococci to the intestinal mucosa and their interference with the adhesion of pathogens, although more than 60% of probiotic preparations in the market contain strains of enterococci. The objective of this study was to investigate if Enterococcus faecium 18C23 has...

journal_title：Applied and environmental microbiology

authors： Jin LZ,Marquardt RR,Zhao X

更新日期：2000-10-01 00:00:00

abstract：:Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This meth...

journal_title：Applied and environmental microbiology

authors： Strovas TJ,Dragavon JM,Hankins TJ,Callis JB,Burgess LW,Lidstrom ME

更新日期：2006-02-01 00:00:00

abstract：:Microbial transformation of the antimelanoma agent betulinic acid was studied. The main objective of this study was to utilize microorganisms as in vitro models to predict and prepare potential mammalian metabolites of this compound. Preparative-scale biotransformation with resting-cell suspensions of Bacillus megater...

journal_title：Applied and environmental microbiology

authors： Chatterjee P,Kouzi SA,Pezzuto JM,Hamann MT

更新日期：2000-09-01 00:00:00

abstract：:In Corynebacterium glutamicum, the ArgR protein, a transcriptional repressor, affects the expression level of the argB gene through binding to its promoter region. The argB promoter region (positions -77 to -25) has been found by in vitro electrophoretic mobility shift assay (EMSA) results and in silico analysis to be...

journal_title：Applied and environmental microbiology

authors： Lee SY,Park JM,Lee JH,Chang ST,Park JS,Kim YH,Min J

更新日期：2011-02-01 00:00:00

abstract：:A Bacillus sp. capable of utilizing phosphite and hypophosphite under anaerobic conditions was isolated from Cape Canerval soil samples. The organism was isolated on a glucose-mineral salts medium with phosphate deleted. Anaerobic cultivation of this isolate resulted in decreases in the hypophosphite or phosphite conc...

journal_title：Applied and environmental microbiology

authors： Foster TL,Winans L Jr,Helms SJ

更新日期：1978-05-01 00:00:00

abstract：:Sterol demethylation inhibitor (DMI) fungicides are widely used to control fungi pathogenic to humans and plants. Resistance to DMIs is mediated either through alterations in the structure of the target enzyme CYP51 (encoding 14alpha-demethylase), through increased expression of the CYP51 gene, or through increased ex...

journal_title：Applied and environmental microbiology

authors： Ma Z,Proffer TJ,Jacobs JL,Sundin GW

更新日期：2006-04-01 00:00:00

abstract：:Shewanella oneidensis MR-1 is a gram-negative facultative anaerobe capable of utilizing a broad range of electron acceptors, including several solid substrates. S. oneidensis MR-1 can reduce Mn(IV) and Fe(III) oxides and can produce current in microbial fuel cells. The mechanisms that are employed by S. oneidensis MR-...

journal_title：Applied and environmental microbiology

authors： Bretschger O,Obraztsova A,Sturm CA,Chang IS,Gorby YA,Reed SB,Culley DE,Reardon CL,Barua S,Romine MF,Zhou J,Beliaev AS,Bouhenni R,Saffarini D,Mansfeld F,Kim BH,Fredrickson JK,Nealson KH

更新日期：2007-11-01 00:00:00

abstract：:Cell extracts of Clostridium thermohydrosulfuricum, an anaerobic bacterium which ferments starch into ethanol at 65 degrees C, contained both pullulanase and glucoamylase activities. The general physiochemical and catalytic properties of these enzyme activities were compared. Pullulanase and glucoamylase activities we...

journal_title：Applied and environmental microbiology

authors： Hyun HH,Zeikus JG

更新日期：1985-05-01 00:00:00

abstract：:In the current study, we adapted and optimized the lambda Red recombineering strategy to genetically manipulate the fastidious insect endosymbiont Sodalis glossinidius. This work greatly facilitates the application of genetics to the study of insect symbionts and should also prove useful in the context of long-awaited...

journal_title：Applied and environmental microbiology

authors： Pontes MH,Dale C

更新日期：2011-03-01 00:00:00

abstract：:A highly selective liquid membrane nitrite microsensor based on the hydrophobic ion-carrier aquocyanocobalt(III)-hepta(2-phenylethyl)-cobrynate is described. The sensor has a tip diameter of 10 to 15 (mu)m. The response is log-linear in freshwater down to 1 (mu)M NO(inf2)(sup-) and in seawater to 10 (mu)M NO(inf2)(sup...

journal_title：Applied and environmental microbiology

authors： De Beer D,Schramm A,Santegoeds CM,Kuhl M

更新日期：1997-03-01 00:00:00

abstract：:This study presents the complete genome sequence of Lactobacillus gasseri ATCC 33323, a neotype strain of human origin and a native species found commonly in the gastrointestinal tracts of neonates and adults. The plasmid-free genome was 1,894,360 bp in size and predicted to encode 1,810 genes. The GC content was 35.3...

journal_title：Applied and environmental microbiology

authors： Azcarate-Peril MA,Altermann E,Goh YJ,Tallon R,Sanozky-Dawes RB,Pfeiler EA,O'Flaherty S,Buck BL,Dobson A,Duong T,Miller MJ,Barrangou R,Klaenhammer TR

更新日期：2008-08-01 00:00:00

abstract：:Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nucle...

journal_title：Applied and environmental microbiology

authors： Quirasco M,López-Munguía A,Remaud-Simeon M,Monsan P,Farrés A

更新日期：1999-12-01 00:00:00

abstract：:The alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When transferred into Lactococcus lactis and Pediococcus acidilactici strains, aga modified the sugar fermentation profile of...

journal_title：Applied and environmental microbiology

authors： Boucher I,Parrot M,Gaudreau H,Champagne CP,Vadeboncoeur C,Moineau S

更新日期：2002-12-01 00:00:00

abstract：:The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutar...

journal_title：Applied and environmental microbiology

authors： Djurdjevic I,Zelder O,Buckel W

更新日期：2011-01-01 00:00:00

abstract：:The esterases and lipases from the α/β hydrolase superfamily exhibit an enormous sequence diversity, fold plasticity, and activities. Here, we present the comprehensive sequence and biochemical analyses of seven distinct esterases and lipases from the metagenome of Lake Arreo, an evaporite karstic lake in Spain (42°46...

journal_title：Applied and environmental microbiology

authors： Martínez-Martínez M,Alcaide M,Tchigvintsev A,Reva O,Polaina J,Bargiela R,Guazzaroni ME,Chicote A,Canet A,Valero F,Rico Eguizabal E,Guerrero Mdel C,Yakunin AF,Ferrer M

更新日期：2013-06-01 00:00:00

abstract：:Various species of Absidia, Aspergillus, Cunninghamella, Trichothecium, Penicillium, and Phanerochaete were found to transform rotenone to one or more metabolites. Two biotransformation products were isolated from a preparative-scale incubation of rotenone with Cunninghamella blakesleeana and identified as 1',2'-dihyd...

journal_title：Applied and environmental microbiology

authors： Sariaslani FS,Rosazza JP

更新日期：1983-02-01 00:00:00

abstract：:During fermentative growth in natural and industrial environments, Saccharomyces cerevisiae must redistribute the available nitrogen from multiple exogenous sources to amino acids in order to suitably fulfill anabolic requirements. To exhaustively explore the management of this complex resource, we developed an advanc...

journal_title：Applied and environmental microbiology

authors： Crépin L,Truong NM,Bloem A,Sanchez I,Dequin S,Camarasa C

更新日期：2017-02-15 00:00:00