Activity and composition of the denitrifying bacterial community respond differently to long-term fertilization.

abstract：:"Dehalococcoides ethenogenes" 195 can reductively dechlorinate tetrachloroethene (PCE) completely to ethene (ETH). When PCE-grown strain 195 was transferred (2% [vol/vol] inoculum) into growth medium amended with trichloroethene (TCE), cis-dichloroethene (DCE), 1,1-DCE, or 1,2-dichloroethane (DCA) as an electron accep...

journal_title：Applied and environmental microbiology

authors： Maymó-Gatell X,Anguish T,Zinder SH

更新日期：1999-07-01 00:00:00

abstract：UNLABELLED:A structurally novel chitinase, Tc-ChiD, was identified from the hyperthermophilic archaeon Thermococcus chitonophagus, which can grow on chitin as the sole organic carbon source. The gene encoding Tc-ChiD contains regions corresponding to a signal sequence, two chitin-binding domains, and a putative catalyt...

journal_title：Applied and environmental microbiology

authors： Horiuchi A,Aslam M,Kanai T,Atomi H

更新日期：2016-05-31 00:00:00

abstract：:The soil isolate Cellvibrio mixtus UQM2294 degraded a variety of polysaccharides including microcrystalline cellulose. Among 6,000 cosmid clones carrying C. mixtus DNA, constructed in Escherichia coli with pHC79, 50 expressed the ability to degrade one or more of the following substrates: carboxymethyl cellulose, chit...

journal_title：Applied and environmental microbiology

authors： Wynne EC,Pemberton JM

更新日期：1986-12-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:The rates of mineralization of phenol, benzoate, benzylamine, p-nitrophenol, and di(2-ethylhexyl) phthalate added to lake water at concentrations ranging from a few picograms to nanograms per milliliter were directly proportional to chemical concentration. The rates were still linear at levels of <1 pg of phenol or p-...

journal_title：Applied and environmental microbiology

authors： Rubin HE,Subba-Rao RV,Alexander M

更新日期：1982-05-01 00:00:00

abstract：:A TAT peptide-delivered molecular beacon was developed and utilized to enumerate murine norovirus 1, a human norovirus (NoV) surrogate, in RAW 264.7 cells. This allowed the detection of a single infective virus within 6 h, a 12-fold improvement in time required for viral detection and quantification compared to that r...

journal_title：Applied and environmental microbiology

authors： Ganguli PS,Chen W,Yates MV

更新日期：2011-08-01 00:00:00

abstract：:High-cell-density fermentation for industrial production of chemicals can impose numerous stresses on cells due to high substrate, product, and by-product concentrations; high osmolarity; reactive oxygen species; and elevated temperatures. There is a need to develop platform strains of industrial microorganisms that a...

journal_title：Applied and environmental microbiology

authors： Lennen RM,Herrgård MJ

更新日期：2014-10-01 00:00:00

abstract：:Alteromonas is a widely distributed genus of marine Gammaproteobacteria, with representatives shown to be key players in diverse processes, including biogeochemical cycling and biofouling of marine substrata. While Alteromonas spp. are early colonizers of copper-based antifouling paints on marine vessels, their mechan...

journal_title：Applied and environmental microbiology

authors： Cusick KD,Polson SW,Duran G,Hill RT

更新日期：2020-01-21 00:00:00

abstract：:The establishment of bacterial communities in two healthy babies was examined for more than the first 10 months of life by monitoring 16S ribosomal DNA (rDNA) diversity in fecal samples by PCR and denaturing gradient gel electrophoresis (DGGE) and by analyzing the sequences of the major ribotypes. DGGE profiles of the...

journal_title：Applied and environmental microbiology

authors： Favier CF,Vaughan EE,De Vos WM,Akkermans AD

更新日期：2002-01-01 00:00:00

abstract：:Escherichia coli ATCC 12806 was exposed to increasing subinhibitory concentrations of three biocides widely used in food industry facilities: trisodium phosphate (TSP), sodium nitrite (SNI), and sodium hypochlorite (SHY). The cultures exhibited an acquired tolerance to biocides (especially to SNI and SHY) after exposu...

journal_title：Applied and environmental microbiology

authors： Capita R,Riesco-Peláez F,Alonso-Hernando A,Alonso-Calleja C

更新日期：2014-02-01 00:00:00

abstract：:In this study, we evaluated the effect of soil clay content on RNA isolation and on quantitative reverse transcription-PCR (qRT-PCR) quantification of microbial gene transcripts. The amount of clay significantly altered RNA isolation yields and qRT-PCR analyses. Recommendations are made for quantifying microbial gene ...

journal_title：Applied and environmental microbiology

authors： Novinscak A,Filion M

更新日期：2011-09-01 00:00:00

abstract：:Wood-degrading fungi use a sequence of oxidative and hydrolytic mechanisms to loosen lignocellulose and then release and metabolize embedded sugars. These temporal sequences have recently been mapped at high resolution using directional growth on wood wafers, revealing previously obscured dynamics as fungi progressive...

journal_title：Applied and environmental microbiology

authors： Presley GN,Panisko E,Purvine SO,Schilling JS

更新日期：2018-08-01 00:00:00

abstract：:Antibody production against aflatoxin B1 was compared in three rabbits and one goat. Titers obtained were 20 times higher in the rabbits than in the goat. The goat antiserum appeared to have a higher degree of cross-reactivity for other aflatoxins and related metabolites than did the rabbit antiserum. ...

journal_title：Applied and environmental microbiology

authors： Gaur PK,El-Nakib O,Chu FS

更新日期：1980-09-01 00:00:00

abstract：:A single type of bacterium, tentatively identified as a member of the genus Bacillus, was isolated from 2 of 20 midguts of Douglas fir tussock moth larvae being fed a diet of fir needles. No bacteria could be isolated from most midguts. Although spherically shaped bodies were present in the food bolus, these bodies, i...

journal_title：Applied and environmental microbiology

authors： Andrews RE,Spence KD

更新日期：1980-11-01 00:00:00

abstract：:Arctic ground squirrels (Urocitellus parryii) are active for a scant 3 to 5 months of the year. During the active season, adult squirrels compete for mates, reproduce, and fatten in preparation for hibernation, while juvenile squirrels, weaned in early July, must grow and acquire sufficient fat to survive their first ...

journal_title：Applied and environmental microbiology

authors： Stevenson TJ,Buck CL,Duddleston KN

更新日期：2014-07-01 00:00:00

abstract：:The adhesion of Pseudomonas fluorescens (ATCC 17552) to nonpolarized and negatively polarized thin films of gold was studied in situ by contrast microscopy using a thin-film electrochemical flow cell. The influence of the electrochemical potential was evaluated at two different ionic strengths (0.01 and 0.1 M NaCl; pH...

journal_title：Applied and environmental microbiology

authors： Busalmen JP,de Sánchez SR

更新日期：2001-07-01 00:00:00

abstract：:Type III protein secretion systems play a key role in the virulence of many pathogenic proteobacteria, but they also occur in nonpathogenic, plant-associated bacteria. Certain type III protein secretion genes (e.g., hrcC) have been found in Pseudomonas sp. strain SBW25 (and other biocontrol pseudomonads), but other ty...

journal_title：Applied and environmental microbiology

authors： Rezzonico F,Défago G,Moënne-Loccoz Y

更新日期：2004-09-01 00:00:00

abstract：:Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

journal_title：Applied and environmental microbiology

authors： Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

更新日期：1980-12-01 00:00:00

abstract：:Photorhabdus luminescens is a bacterium which is mutualistic with entomophagous nematodes and which secretes high-molecular-weight toxin complexes following its release into the insect hemocoel upon nematode invasion. Thus, unlike other protein toxins from Bacillus thuringiensis (delta-endotoxins and Vip's), P. lumine...

journal_title：Applied and environmental microbiology

authors： Blackburn M,Golubeva E,Bowen D,Ffrench-Constant RH

更新日期：1998-08-01 00:00:00

abstract：:Wieners and sausages were prepared which contained the most heat-tolerant representative of the Mycobacterium avium-Mycobacterium intracellulare complex we were able to obtain. They also were prepared with infected tissues obtained from tuberculous swine. Processing conditions were as varied as possible. Neither incor...

journal_title：Applied and environmental microbiology

authors： Merkal RS,Crawford JA,Whipple DL

更新日期：1979-11-01 00:00:00

abstract：:A defined medium for Aquaspirillum serpens VHL allows the replacement of the complex media now in use. It was developed by batch culture methods but supports growth in continuous culture. A basal salts medium supplemented with L-aspartic acid, L-alanine, and L-glutamic acid provided the best growth (turbidity), as lon...

journal_title：Applied and environmental microbiology

authors： Whitby GE,Murray RG

更新日期：1980-01-01 00:00:00

abstract：:Aflatoxin B1 in a 5-micrograms/ml water solution was sensitive to ionizing radiation. Inactivation was assayed by the Ames microsome mutagenicity test and confirmed by thin-layer chromatography. Destruction of aflatoxin B1 had already begun at 2.5 kilograms (kGy; 1 kGy = 0.1 Mrad), but a dose exceeding 10 kGy was nece...

journal_title：Applied and environmental microbiology

authors： Van Dyck PJ,Tobback P,Feyes M,van de Voorde H

更新日期：1982-06-01 00:00:00

abstract：:Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

journal_title：Applied and environmental microbiology

authors： Hyman MR,Murton IB,Arp DJ

更新日期：1988-12-01 00:00:00

abstract：:Escherichia coli is one of the most widely used host microorganisms for recombinant protein expression and metabolic engineering, but it cannot efficiently secrete recombinant proteins to extracellular space. Here, extracellular protein secretion was enhanced in E. coli by deleting two d,d-carboxypeptidase genes (dacA...

journal_title：Applied and environmental microbiology

authors： Yang H,Lu X,Hu J,Chen Y,Shen W,Liu L

更新日期：2018-10-01 00:00:00

abstract：:Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

journal_title：Applied and environmental microbiology

authors： Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

更新日期：2003-02-01 00:00:00

abstract：:Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infec...

journal_title：Applied and environmental microbiology

authors： Miya S,Takahashi H,Ishikawa T,Fujii T,Kimura B

更新日期：2010-05-01 00:00:00

abstract：:Chlamydiae are important pathogens of humans and animals but diagnosis of chlamydial infections is still hampered by inadequate detection methods. Fluorescence in situ hybridization (FISH) using rRNA-targeted oligonucleotide probes is widely used for the investigation of uncultured bacteria in complex microbial commun...

journal_title：Applied and environmental microbiology

authors： Poppert S,Essig A,Marre R,Wagner M,Horn M

更新日期：2002-08-01 00:00:00

abstract：:We report that two species of basidiomycete fungi (Polyporus versicolor and Poria monticola) grow in minimal liquid or solid medium when supplemented with crushed lignite coal. The fungi also grow directly on crushed lignite coal. The growth of both fungi was observed qualitatively as the production and extension of h...

journal_title：Applied and environmental microbiology

authors： Cohen MS,Gabriele PD

更新日期：1982-07-01 00:00:00

abstract：:The widespread use and relative persistence of s-triazine compounds such as atrazine and simazine have led to increasing concern about environmental contamination by these compounds. Few microbial isolates capable of transforming substituted s-triazines have been identified. Rhodococcus corallinus NRRL B-15444 has pre...

journal_title：Applied and environmental microbiology

authors： Mulbry WW

更新日期：1994-02-01 00:00:00

abstract：:Chemolithotrophic ammonium-oxidizing and nitrite-oxidizing bacteria including Nitrosomonas europaea, Nitrosococcus oceanus, Nitrobacter sp., Nitiospina gracilis, and Nitrococcus mobilis were examined as to their ability to oxidize methane in the absence of ammonium or nitrite. All ammonium oxidizers tested had the abi...

journal_title：Applied and environmental microbiology

authors： Jones RD,Morita RY

更新日期：1983-02-01 00:00:00