New method for large-scale growth; and concentration of the Epstein-Barr viruses.

abstract：:A betaproteobacterium within the family Rhodocyclaceae previously identified as a pyrene degrader via stable-isotope probing (SIP) of contaminated soil (designated pyrene group 1 or PG1) was cultivated as the dominant member of a mixed bacterial culture. A metagenomic library was constructed, and the largest contigs w...

journal_title：Applied and environmental microbiology

authors： Singleton DR,Hu J,Aitken MD

更新日期：2012-05-01 00:00:00

abstract：:A biologically active metabolite was found in crude extracts of Curvularia lunata (Wakker) Boedijn (ATCC 34690) isolated from decayed tissues of litchi fruit (Litchi chinensis Sonn.). The fungus was grown on a shredded wheat-yeast extract-sucrose medium, and cultures were extracted with chloroform after 3 weeks of gro...

journal_title：Applied and environmental microbiology

authors： Wells JM,Cole RJ,Cutler HC,Spalding DH

更新日期：1981-04-01 00:00:00

abstract：:Similar populations of hypochlorite-treated spores were enumerated from two crops of Clostridium botulinum 12885A produced by the same procedure; however, germination required different L-alanine concentrations. Lactate permitted the germination of spores from both crops with suboptimal L-alanine concentrations. The d...

journal_title：Applied and environmental microbiology

authors： Foegeding PM,Busta FF

更新日期：1983-04-01 00:00:00

abstract：:A database was probed with artificial neural network (ANN) and multivariate logistic regression (MLR) models to investigate the efficacy of predicting PCR-identified human adenovirus (ADV), Norwalk-like virus (NLV), and enterovirus (EV) presence or absence in shellfish harvested from diverse countries in Europe (Spain...

journal_title：Applied and environmental microbiology

authors： Brion G,Viswanathan C,Neelakantan TR,Lingireddy S,Girones R,Lees D,Allard A,Vantarakis A

更新日期：2005-09-01 00:00:00

abstract：:The initiation of methanogenesis in refuse occurs under high volatile fatty acid (VFA) concentration and low pH (5.5 to 6.25), which generally are reported to inhibit methanogenic Archaea. One hypothesized mechanism for the initiation of methanogenesis in refuse decomposition is the presence of pH-neutral niches withi...

journal_title：Applied and environmental microbiology

authors： Staley BF,de Los Reyes FL 3rd,Barlaz MA

更新日期：2011-04-01 00:00:00

abstract：:1,2-Dichloroethane (1,2-DCA) and 1,2-dibromoethane (ethylene dibromide [EDB]) contaminate groundwater at many hazardous waste sites. The objectives of this study were to measure yields, maximum specific growth rates (μ), and half-saturation coefficients (K(S)) in enrichment cultures that use 1,2-DCA and EDB as termina...

journal_title：Applied and environmental microbiology

authors： Yu R,Peethambaram HS,Falta RW,Verce MF,Henderson JK,Bagwell CE,Brigmon RL,Freedman DL

更新日期：2013-02-01 00:00:00

abstract：:We used global transcript analyses and mutant studies to investigate the pathways that impact H(2) production in the photosynthetic bacterium Rhodobacter sphaeroides. We found that H(2) production capacity is related to the levels of expression of the nitrogenase and hydrogenase enzymes and the enzymes of the Calvin-B...

journal_title：Applied and environmental microbiology

authors： Kontur WS,Ziegelhoffer EC,Spero MA,Imam S,Noguera DR,Donohue TJ

更新日期：2011-10-01 00:00:00

abstract：:The marine diatom Thallasiosira pseudonanna (3H) and several bacteria associated with it were isolated from batch cultures at the University of Delaware mariculture facility. The interaction between the algae and each of the bacteria was investigated. One of the isolates, T827/2B (Pseudomonas sp.), was incapable of su...

journal_title：Applied and environmental microbiology

authors： Baker KH,Herson DS

更新日期：1978-04-01 00:00:00

abstract：:We examined the harmful side effects on indigenous soil microorganisms of two organic solvents, acetone and dichloromethane, that are normally used for spiking of soil with polycyclic aromatic hydrocarbons for experimental purposes. The solvents were applied in two contamination protocols to either the whole soil samp...

journal_title：Applied and environmental microbiology

authors： Brinch UC,Ekelund F,Jacobsen CS

更新日期：2002-04-01 00:00:00

abstract：:Staphylococcus aureus Cowan 1 cells were armed with anti-flagellar (anti-H) antibody produced in rabbits immunized with flagellar core protein prepared from Vibrio vulnificus. This reagent was assessed by coagglutination for its capacity to agglutinate and identify V. vulnificus. A species-specific H antigen is expres...

journal_title：Applied and environmental microbiology

authors： Simonson J,Siebeling RJ

更新日期：1986-12-01 00:00:00

abstract：:Anaerobic degradation of polycyclic aromatic hydrocarbons has been investigated mostly with naphthalene as a model compound. Naphthalene degradation by sulfate-reducing bacteria proceeds via carboxylation to 2-naphthoic acid, formation of a coenzyme A thioester, and subsequent reduction to 5,6,7,8-tetrahydro-2-naphtho...

journal_title：Applied and environmental microbiology

authors： Weyrauch P,Heker I,Zaytsev AV,von Hagen CA,Arnold ME,Golding BT,Meckenstock RU

更新日期：2020-07-20 00:00:00

abstract：:Multicellular communities produced by Bacillus subtilis can adopt sliding or swarming to translocate over surfaces. While sliding is a flagellum-independent motility produced by the expansive forces in a growing colony, swarming requires flagellar functionality and is characterized by the appearance of hyperflagellate...

journal_title：Applied and environmental microbiology

authors： Ghelardi E,Salvetti S,Ceragioli M,Gueye SA,Celandroni F,Senesi S

更新日期：2012-09-01 00:00:00

abstract：:Resistance to bile is a prerequisite property of the gastrointestinal bacterial flora. Bile acids are powerful detergents, and resistance to sodium dodecyl sulfate (SDS) has therefore often been considered relevant to studies of bile resistance. We have studied the effects of bovine bile (BB) and SDS on Enterococcus f...

journal_title：Applied and environmental microbiology

authors： Solheim M,Aakra A,Vebø H,Snipen L,Nes IF

更新日期：2007-09-01 00:00:00

abstract：:Laying hens were individually caged at 20 weeks of age and tested for fecal excretion of Campylobacter jejuni (minimum level of detection was 100 cfu/g) during a 42-week period. Peak rates of C. jejuni isolation (approximately 25% of hens positive) occurred at two different times, in October and in late April to early...

journal_title：Applied and environmental microbiology

authors： Doyle MP

更新日期：1984-03-01 00:00:00

abstract：:Given the interest in Bacteroides species as microbial source tracking (MST) markers, and the limited knowledge of the survival of Bacteroides species in the environment, here we examine the survival of Bacteroides fragilis, B. thetaiotaomicron, and environmental species of Bacteroides by use of culture techniques and...

journal_title：Applied and environmental microbiology

authors： Ballesté E,Blanch AR

更新日期：2010-11-01 00:00:00

abstract：:Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typi...

journal_title：Applied and environmental microbiology

authors： Varshney AK,Mediavilla JR,Robiou N,Guh A,Wang X,Gialanella P,Levi MH,Kreiswirth BN,Fries BC

更新日期：2009-11-01 00:00:00

abstract：:Survival studies were conducted on Legionella pneumophila cells that had been grown intracellularly in Acanthamoeba polyphaga and then exposed to polyhexamethylene biguanide (PHMB), benzisothiazolone (BIT), and 5-chloro-N-methylisothiazolone (CMIT). Susceptibilities were also determined for L. pneumophila grown under ...

journal_title：Applied and environmental microbiology

authors： Barker J,Brown MR,Collier PJ,Farrell I,Gilbert P

更新日期：1992-08-01 00:00:00

abstract：:Bdellovibrios capable of axenic growth grow in a cell-free medium at a rate considerably lower than that attainable in a two-membered culture with Escherichia coli. The axenic growth rate may be improved either by adjustment of the osmosity of the medium or by the addition of low concentrations of spermine. ...

journal_title：Applied and environmental microbiology

authors： Varon M,Seijffers J

更新日期：1977-05-01 00:00:00

abstract：:The nucleotide sequence of a new plasmid pCNB1 from Comamonas sp. strain CNB-1 that degrades 4-chloronitrobenzene (4CNB) was determined. pCNB1 belongs to the IncP-1beta group and is 91,181 bp in length. A total of 95 open reading frames appear to be involved in (i) the replication, maintenance, and transfer of pCNB1; ...

journal_title：Applied and environmental microbiology

authors： Ma YF,Wu JF,Wang SY,Jiang CY,Zhang Y,Qi SW,Liu L,Zhao GP,Liu SJ

更新日期：2007-07-01 00:00:00

abstract：:The different serotyping systems, based on thermostable O antigens, reported for Vibrio anguillarum and V. ordalii were compared by quantitative agglutination, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and subsequent silver staining or Western blotting (immunoblotting) of purified lipopolysaccharide (...

journal_title：Applied and environmental microbiology

authors： Grisez L,Ollevier F

更新日期：1995-12-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

journal_title：Applied and environmental microbiology

authors： Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

更新日期：2007-10-01 00:00:00

abstract：:Oil field bacteria were characterized by cloning and sequencing of PCR-amplified 16S rRNA genes. A variety of gram-negative, sulfate-reducing bacteria was detected (16 members of the family Desulfovibrionaceae and 8 members of the family Desulfobacteriaceae). In contrast, a much more limited number of anaerobic, ferme...

journal_title：Applied and environmental microbiology

authors： Voordouw G,Armstrong SM,Reimer MF,Fouts B,Telang AJ,Shen Y,Gevertz D

更新日期：1996-05-01 00:00:00

abstract：:During microaerophilic growth of magnetic spirillum MS-1 on tartrate and nitrate, a maximal cell density was obtained at an initial oxygen partial pressure of 17 Pa. A transient accumulation of nitrous oxide and a 1:2 (mol/mol) stoichiometry between tartrate oxidation and nitrate reduction were observed, indicating th...

journal_title：Applied and environmental microbiology

authors： Escalante-Semerena JC,Blakemore RP,Wolfe RS

更新日期：1980-08-01 00:00:00

abstract：:Two different DNA fragments, one of 2.9 kilobases and the other of 5.1 kilobases, were cloned from Phytophthora citrophthora and showed no homology with DNA from plants and other related fungi. These DNA probes hybridized with DNA from 12 different P. citrophthora isolates obtained from a variety of hosts but did not ...

journal_title：Applied and environmental microbiology

authors： Goodwin PH,Kirkpatrick BC,Duniway JM

更新日期：1990-03-01 00:00:00

abstract：:Integrated air sampling and PCR-based methods for detecting airborne fungal spores, using Penicillium roqueforti as a model fungus, are described. P. roqueforti spores were collected directly into Eppendorf tubes using a miniature cyclone-type air sampler. They were then suspended in 0.1% Nonidet P-40, and counted usi...

journal_title：Applied and environmental microbiology

authors： Williams RH,Ward E,McCartney HA

更新日期：2001-06-01 00:00:00

abstract：:Clostridium perfringens food poisoning is caused mainly by enterotoxigenic type A isolates that typically possess high spore heat resistance. Previous studies have shown that alpha/beta-type small, acid-soluble proteins (SASP) play a major role in the resistance of Bacillus subtilis and C. perfringens spores to moist ...

journal_title：Applied and environmental microbiology

authors： Paredes-Sabja D,Sarker N,Setlow B,Setlow P,Sarker MR

更新日期：2008-06-01 00:00:00

abstract：:We developed a novel quantitative real-time PCR (Q-PCR) method for the soil actinomycete Rhodococcus equi, an important horse pathogen and emerging human pathogen. Species-specific quantification was achieved by targeting the chromosomal monocopy gene choE, universally conserved in R. equi. The choE Q-PCR included an ...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Lewis DA,Ocampo-Sosa AA,Fogarty U,Makrai L,Navas J,Scortti M,Hernández M,Vázquez-Boland JA

更新日期：2006-06-01 00:00:00

abstract：:In vivo hydrolysis of inulin and sucrose was examined in selected yeasts of the genus Kluyveromyces. Cells, grown in sucrose-limited chemostat cultures, were subjected to treatments for the removal of inulinase, the enzyme responsible for the hydrolysis of both inulin and sucrose. The effects of these treatments were ...

journal_title：Applied and environmental microbiology

authors： Rouwenhorst RJ,Ritmeester WS,Scheffers WA,Van Dijken JP

更新日期：1990-11-01 00:00:00

abstract：:In this study, we developed a new mariner-based transposition system for Listeria monocytogenes. The mariner-based system has a high rate of transposition and a low rate of plasmid retention, and transposition is very random, making it an ideal tool for high-throughput transposon mutagenesis in L. monocytogenes. ...

journal_title：Applied and environmental microbiology

authors： Cao M,Bitar AP,Marquis H

更新日期：2007-04-01 00:00:00

abstract：:The bioconversion of sugars present in wood hemicellulose to 2,3-butanediol by Klebsiella pneumoniae grown on high sugar concentrations was investigated. When K. pneumoniae was grown under finite air conditions in the presence of added acetic acid, 50 g of D-glucose and D-xylose per liter could be converted to 25 and ...

journal_title：Applied and environmental microbiology

authors： Yu EK,Saddler JN

更新日期：1983-09-01 00:00:00