Rapid serological identification of Vibrio vulnificus by anti-H coagglutination.

abstract：:The haloalkane dehalogenase enzyme DmmA was identified by marine metagenomic screening. Determination of its crystal structure revealed an unusually large active site compared to those of previously characterized haloalkane dehalogenases. Here we present a biochemical characterization of this interesting enzyme with e...

journal_title：Applied and environmental microbiology

authors： Buryska T,Babkova P,Vavra O,Damborsky J,Prokop Z

更新日期：2018-01-02 00:00:00

abstract：:The metagenomic approach provides direct access to diverse unexplored genomes, especially from uncultivated bacteria in a given environment. This diversity can conceal many new biosynthetic pathways. Type I polyketide synthases (PKSI) are modular enzymes involved in the biosynthesis of many natural products of industr...

journal_title：Applied and environmental microbiology

authors： Ginolhac A,Jarrin C,Gillet B,Robe P,Pujic P,Tuphile K,Bertrand H,Vogel TM,Perrière G,Simonet P,Nalin R

更新日期：2004-09-01 00:00:00

abstract：:Elevated winter losses of managed honeybee colonies are a major concern, but the underlying mechanisms remain controversial. Among the suspects are the parasitic mite Varroa destructor, the microsporidian Nosema ceranae, and associated viruses. Here we hypothesize that pathogens reduce the life expectancy of winter be...

journal_title：Applied and environmental microbiology

authors： Dainat B,Evans JD,Chen YP,Gauthier L,Neumann P

更新日期：2012-02-01 00:00:00

abstract：:Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

journal_title：Applied and environmental microbiology

authors： Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

更新日期：2014-11-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Physical agents, such as low electric voltage and current, have recently gained attention for antimicrobial treatment due to their bactericidal capability. Although microampere electric current was shown to suppress the growth of bacteria, it remains unclear to what extent the microampere current damaged the bacterial...

journal_title：Applied and environmental microbiology

authors： Krishnamurthi VR,Rogers A,Peifer J,Niyonshuti II,Chen J,Wang Y

更新日期：2020-08-03 00:00:00

abstract：:[This corrects the article on p. 507 in vol. 30.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-12-01 00:00:00

abstract：:We developed a self-formed adaptor PCR (termed SEFA PCR) which can be used for chromosome walking. Most of the amplified flanking sequences were longer than 2.0 kb, and some were as long as 6.0 kb. SEFA PCR is simple and efficient and should have broad applications in the isolation of unknown sequences in complex geno...

journal_title：Applied and environmental microbiology

authors： Wang S,He J,Cui Z,Li S

更新日期：2007-08-01 00:00:00

abstract：:Overgrowth by spreading molds such as Rhizopus and Mucor species is a problem with fungal enumeration media used for foods. Thirty-one antifungal compounds were surveyed for their ability to selectively inhibit such fungi while allowing growth of mycotoxigenic molds and other species of significance in food spoilage. ...

journal_title：Applied and environmental microbiology

authors： King AD Jr,Hocking AD,Pitt JI

更新日期：1979-05-01 00:00:00

abstract：:Next-generation sequencing (NGS) opens up exciting possibilities for improving our knowledge of environmental microbial diversity, allowing rapid and cost-effective identification of both cultivated and uncultivated microorganisms. However, library preparation, sequencing, and analysis of the results can provide inacc...

journal_title：Applied and environmental microbiology

authors： Cruaud P,Vigneron A,Lucchetti-Miganeh C,Ciron PE,Godfroy A,Cambon-Bonavita MA

更新日期：2014-08-01 00:00:00

abstract：:Laboratory batch tests indicate that addition of sterile municipal sewage biosolids to clay soil from four depths increases the numbers of Escherichia coli isolates recoverable in EC-MUG broth (EC broth with 4-methylumbelliferyl-beta-glucuronide). This effect was most marked for the deeper soil layers, with increases ...

journal_title：Applied and environmental microbiology

authors： Unc A,Gardner J,Springthorpe S

更新日期：2006-03-01 00:00:00

abstract：:Noroviruses (NoV) annually cause millions of cases of gastrointestinal disease in the United States. NoV are associated with raw shellfish outbreaks, particularly oysters, which are thought to bioaccumulate NoV particles during the filter-feeding process. NoV outbreaks, however, have also been known to occur from othe...

journal_title：Applied and environmental microbiology

authors： Gandhi KM,Mandrell RE,Tian P

更新日期：2010-12-01 00:00:00

abstract：:We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuat...

journal_title：Applied and environmental microbiology

authors： Willyerd KL,Kemp AM,Dawe AL

更新日期：2009-08-01 00:00:00

abstract：:Diploscapter, a thermotolerant, free-living soil bacterial-feeding nematode commonly found in compost, sewage, and agricultural soil in the United States, was studied to determine its potential role as a vehicle of Salmonella enterica serotype Poona, enterohemorrhagic Escherichia coli O157:H7, and Listeria monocytogen...

journal_title：Applied and environmental microbiology

authors： Gibbs DS,Anderson GL,Beuchat LR,Carta LK,Williams PL

更新日期：2005-05-01 00:00:00

abstract：:Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

journal_title：Applied and environmental microbiology

authors： Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

更新日期：2019-01-09 00:00:00

abstract：:Hadal environments sustain diverse microorganisms. A few studies have investigated hadal microbial communities consisting of free-living or particle-associated bacteria and archaea. However, animal-associated microbial communities in hadal environments remain largely unexplored, and comparative analyses of animal gut ...

journal_title：Applied and environmental microbiology

authors： Zhang W,Watanabe HK,Ding W,Lan Y,Tian RM,Sun J,Chen C,Cai L,Li Y,Oguri K,Toyofuku T,Kitazato H,Drazen JC,Bartlett D,Qian PY

更新日期：2018-12-13 00:00:00

abstract：:The effect of yeast hulls (yeast ghosts) on sluggish or stuck white wine fermentations was studied. The enhancing effect on yeast growth and fermentation rate displayed by the hulls was shown to be similar to the effect provided by lipid extract from the same hulls. Unsaturated fatty acids and sterols were incorporate...

journal_title：Applied and environmental microbiology

authors： Munoz E,Ingledew WM

更新日期：1989-06-01 00:00:00

abstract：:A novel, semicontinuous solid-phase fermentation system was used to produce fuel ethanol from sweet sorghum. The process was at an intermediate scale. In the process, dried and shredded sweet sorghum was rehydrated to 70% moisture, acidified to pH 2.0 to 3.0, and either pasteurized (12 h at 70 to 80 degrees C) or not ...

journal_title：Applied and environmental microbiology

authors： Gibbons WR,Westby CA,Dobbs TL

更新日期：1986-01-01 00:00:00

abstract：:The subsurface of a tidal-flat sediment was analyzed down to 360 cm in depth by molecular and geochemical methods. A community structure analysis of all three domains of life was performed using domain-specific PCR followed by denaturing gradient gel electrophoresis analysis and sequencing of characteristic bands. The...

journal_title：Applied and environmental microbiology

authors： Wilms R,Sass H,Köpke B,Köster J,Cypionka H,Engelen B

更新日期：2006-04-01 00:00:00

abstract：:Bacteriovorous protozoa harboring symbiotic algae are abundant in aquatic ecosystems, yet despite a recent interest in protozoan bacterivory, the influence of light on their ingestion rates has not been investigated. In this study, Paramecium bursaria containing endosymbiotic Chlorella was tested for the effect of lig...

journal_title：Applied and environmental microbiology

authors： Berk SG,Parks LH,Ting RS

更新日期：1991-08-01 00:00:00

abstract：:Heterotrophic bacteria in the SAR11 and Roseobacter lineages shape the marine carbon, nitrogen, phosphorous, and sulfur cycles, yet they do so having adopted divergent ecological strategies. Currently, it is unknown whether these globally significant groups partition into specific niches with respect to micronutrients...

journal_title：Applied and environmental microbiology

authors： Hogle SL,Thrash JC,Dupont CL,Barbeau KA

更新日期：2016-01-04 00:00:00

abstract：:The anaerobic bacterium Eubacterium lentum, a common constituent of the intestinal microflora, inactivates digoxin by reducing the unsaturated lactone ring. Reduction of the cardiac glycoside by growing cultures of E. lentum ATCC 25559 proceeded in a stereospecific manner, with the 20R-dihydrodigoxin constituting more...

journal_title：Applied and environmental microbiology

authors： Robertson LW,Chandrasekaran A,Reuning RH,Hui J,Rawal BD

更新日期：1986-06-01 00:00:00

abstract：UNLABELLED:Stress-induced abdominal dysfunction is an attractive target for probiotics. To investigate the effects of the probiotic Lactobacillus casei strain Shirota on abdominal dysfunction, a double-blind, placebo-controlled trial was conducted with healthy medical students undertaking an authorized nationwide exami...

journal_title：Applied and environmental microbiology

authors： Kato-Kataoka A,Nishida K,Takada M,Kawai M,Kikuchi-Hayakawa H,Suda K,Ishikawa H,Gondo Y,Shimizu K,Matsuki T,Kushiro A,Hoshi R,Watanabe O,Igarashi T,Miyazaki K,Kuwano Y,Rokutan K

更新日期：2016-05-31 00:00:00

abstract：:Growth, survival, and pathogenicity of Klebsiella growing in and on environmental foci were examined. Total coliforms present in raw wastes from pulp mills were in excess of 10(5)/ml, and 60 to 80% were Klebsiella. Fecal coliform counts ranged from 10(1) to 10(5)/ml. Klebsiella isolates from industrial effluents and a...

journal_title：Applied and environmental microbiology

authors： Knittel MD,Seidler RJ,Eby C,Cabe LM

更新日期：1977-11-01 00:00:00

abstract：:Rhodococcus equi is a leading cause of severe pneumonia in foals. Standard treatment is dual antimicrobial therapy with a macrolide and rifampin, but the emergence of macrolide- and rifampin-resistant R. equi isolates is an increasing problem. The objective of this study was to determine the effect of macrolide and/or...

journal_title：Applied and environmental microbiology

authors： Willingham-Lane JM,Berghaus LJ,Berghaus RD,Hart KA,Giguère S

更新日期：2019-03-22 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:Two different endoxylanases (1,4-beta-D-xylan xylanohydrolases, EC 3.2.1.8), designated 1 and 2, have been purified by column chromatography to apparent homogeneity from the nonsedimentable extracellular culture fluid of the strictly anaerobic, ruminal bacterium Fibrobacter succinogenes S85 grown on crystalline cellul...

journal_title：Applied and environmental microbiology

authors： Matte A,Forsberg CW

更新日期：1992-01-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:Several formulations of brilliant green agar with an added H2S indicator were evaluated. Results were optimum with variations of a basic formula consisting of 40 g of tryptic soy agar (Difco), 8 g of lactose, 8 g of sucrose, 80 mg of phenol red, 1 g of sulfanilamide, 1.5 g of ferric ammonium citrate, 5 g of sodium thi...

journal_title：Applied and environmental microbiology

authors： Moats WA,Kinner JA

更新日期：1976-03-01 00:00:00

abstract：:A multilocus enzyme electrophoresis analysis of 96 strains of the salmonellae distinguished 80 electrophoretic types (ETs) and placed them in eight groups, seven of which correspond precisely to the seven taxonomic groups (I, II, IIIa, IIIb, IV, V, and VI) previously defined on the basis of biotype and genomic DNA hyb...

journal_title：Applied and environmental microbiology

authors： Boyd EF,Wang FS,Whittam TS,Selander RK

更新日期：1996-03-01 00:00:00