Dead or alive: deformed wing virus and Varroa destructor reduce the life span of winter honeybees.

abstract：:Three predominant ruminal cellulolytic bacteria (Fibrobacter succinogenes S85, Ruminococcus flavefaciens FD-1, and Ruminococcus albus 7) were grown in different binary combinations to determine the outcome of competition in either cellulose-excess batch culture or in cellulose-limited continuous culture. Relative popu...

journal_title：Applied and environmental microbiology

authors： Shi Y,Odt CL,Weimer PJ

更新日期：1997-02-01 00:00:00

abstract：:The goal of this study was to determine how enteric viruses persist within shellfish tissues. Several lines of novel evidence show that phagocytic blood cells (hemocytes) of Eastern oysters (Crassostrea virginica) play an important role in the retention of virus particles. Our results demonstrated an association of vi...

journal_title：Applied and environmental microbiology

authors： Provost K,Dancho BA,Ozbay G,Anderson RS,Richards GP,Kingsley DH

更新日期：2011-12-01 00:00:00

abstract：:A defined substrate method was developed to simultaneously enumerate total coliforms and Escherichia coli from drinking waters without the need for confirmatory or completed tests. It is a new method based on technology that uses a hydrolyzable substrate as a specific indicator-nutrient for the target microbes. No equ...

journal_title：Applied and environmental microbiology

authors： Edberg SC,Allen MJ,Smith DB

更新日期：1988-06-01 00:00:00

abstract：:A dot blot hybridization procedure with 32P-labeled whole chromosomal DNA of the type strains as probes was developed as a rapid and simple method for identification of intestinal Bacteroides species. Bacterial cells were fixed onto membrane filters by slight suction, treated with 0.5 N NaOH, and hybridized with these...

journal_title：Applied and environmental microbiology

authors： Morotomi M,Ohno T,Mutai M

更新日期：1988-05-01 00:00:00

abstract：:Fermentation of the pentose sugar xylose to ethanol in lignocellulosic biomass would make bioethanol production economically more competitive. Saccharomyces cerevisiae, an efficient ethanol producer, can utilize xylose only when expressing the heterologous genes XYL1 (xylose reductase) and XYL2 (xylitol dehydrogenase)...

journal_title：Applied and environmental microbiology

authors： Johansson B,Christensson C,Hobley T,Hahn-Hägerdal B

更新日期：2001-09-01 00:00:00

abstract：:Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. ...

journal_title：Applied and environmental microbiology

authors： Corbett LL,Parker DL

更新日期：1976-12-01 00:00:00

abstract：:Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...

journal_title：Applied and environmental microbiology

authors： Petersen JM,Schriefer ME,Gage KL,Montenieri JA,Carter LG,Stanley M,Chu MC

更新日期：2004-06-01 00:00:00

abstract：:The harvesting of rainwater is gaining acceptance among many governmental authorities in countries such as Australia, Germany, and South Africa, among others. However, conflicting reports on the microbial quality of harvested rainwater have been published. To monitor the presence of potential pathogenic bacteria durin...

journal_title：Applied and environmental microbiology

authors： Dobrowsky PH,De Kwaadsteniet M,Cloete TE,Khan W

更新日期：2014-04-01 00:00:00

abstract：:The existence of airborne mycotoxins in mold-contaminated buildings has long been hypothesized to be a potential occupant health risk. However, little work has been done to demonstrate the presence of these compounds in such environments. The presence of airborne macrocyclic trichothecene mycotoxins in indoor environm...

journal_title：Applied and environmental microbiology

authors： Brasel TL,Martin JM,Carriker CG,Wilson SC,Straus DC

更新日期：2005-11-01 00:00:00

abstract：:Strain selection and improvement in the baker's yeast industry have aimed to increase the speed of maltose fermentation in order to increase the leavening activity of industrial baking yeast. We identified two groups of baker's strains of Saccharomyces cerevisiae that can be distinguished by the mode of regulation of ...

journal_title：Applied and environmental microbiology

authors： Higgins VJ,Braidwood M,Bell P,Bissinger P,Dawes IW,Attfield PV

更新日期：1999-02-01 00:00:00

abstract：:The diversity of the predominant bacteria in the human gastrointestinal tract was studied by using 16S rRNA-based approaches. PCR amplicons of the V6 to V8 regions of fecal 16S rRNA and ribosomal DNA (rDNA) were analyzed by temperature gradient gel electrophoresis (TGGE). TGGE of fecal 16S rDNA amplicons from 16 indiv...

journal_title：Applied and environmental microbiology

authors： Zoetendal EG,Akkermans AD,De Vos WM

更新日期：1998-10-01 00:00:00

abstract：:The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

journal_title：Applied and environmental microbiology

authors： Gagnevin L,Leach JE,Pruvost O

更新日期：1997-01-01 00:00:00

abstract：:A lambda recombinant phage expressing beta-mannanase activity in Escherichia coli has been isolated from a genomic library of the extremely thermophilic anaerobe "Caldocellum saccharolyticum." The gene was cloned into pBR322 on a 5-kb BamHI fragment, and its location was obtained by deletion analysis. The sequence of ...

journal_title：Applied and environmental microbiology

authors： Lüthi E,Jasmat NB,Grayling RA,Love DR,Bergquist PL

更新日期：1991-03-01 00:00:00

abstract：:Most Shiga toxin-producing Escherichia coli (STEC) strains associated with severe disease, such as hemolytic-uremic syndrome (HUS), carry large enterohemolysin-encoding (ehxA) plasmids, e.g., pO157 and pO103, that contribute to STEC clinical manifestations. Six ehxA subtypes (A through F) exist that phylogenetically c...

journal_title：Applied and environmental microbiology

authors： Lorenz SC,Monday SR,Hoffmann M,Fischer M,Kase JA

更新日期：2016-10-14 00:00:00

abstract：:Halophenols such as 2,4-dibromophenol (DBP) occur naturally in some marine sediments, as a consequence of various animal and algal activities. In an earlier study, DBP was observed in the burrow microenvironment of the hemichordate Saccoglossus kowalewskii. At the concentrations found in the burrow lining, aerobic res...

journal_title：Applied and environmental microbiology

authors： King GM

更新日期：1988-12-01 00:00:00

abstract：:The sediment-water interface in Halifax Harbor supports a microbial population of 6.95 x 10 cells per g (dry weight). As determined by the standard technique of suspending subsamples in filtered seawater, the uptake of added glutamic acid by this population is 113.5 ng g (dry weight) h. An alternate technique was deve...

journal_title：Applied and environmental microbiology

authors： Novitsky JA

更新日期：1983-06-01 00:00:00

abstract：:Two genetically engineered microorganisms (GEMs), Pseudomonas sp. strain B13 FR1(pFRC20P) (FR120) and Pseudomonas putida KT2440(pWWO-EB62) (EB62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. FR120 cont...

journal_title：Applied and environmental microbiology

authors： Nüsslein K,Maris D,Timmis K,Dwyer DF

更新日期：1992-10-01 00:00:00

abstract：:The ability of Escherichia coli MV1184 to accumulate inorganic phosphate (Pi) was enhanced by manipulating the genes involved in the transport and metabolism of Pi. The high-level Pi accumulation was achieved by modifying the genetic regulation and increasing the dosage of the E. coli genes encoding polyphosphate kina...

journal_title：Applied and environmental microbiology

authors： Kato J,Yamada K,Muramatsu A,Hardoyo,Ohtake H

更新日期：1993-11-01 00:00:00

abstract：:The acid-insoluble metal sulfides FeS2, MoS2, and WS2 are chemically attacked by iron(III) hexahydrate ions, generating thiosulfate, which is oxidized to sulfuric acid. Other metal sulfides are attacked by iron(III) ions and by protons, resulting in the formation of elemental sulfur via intermediary polysulfides. Sulf...

journal_title：Applied and environmental microbiology

authors： Schippers A,Sand W

更新日期：1999-01-01 00:00:00

abstract：:Ergosterol is a membrane component specific to fungi that can be used to estimate fungal biomass using appropriate factors of conversion. Our objectives were to determine the limits of use of ergosterol content as a measure of biomass for aquatic hyphomycetes, and to evaluate a previously established ergosterol-to-bio...

journal_title：Applied and environmental microbiology

authors： Charcosset JY,Chauvet E

更新日期：2001-05-01 00:00:00

abstract：:The polymerase chain reaction (PCR) and a radiolabeled oligonucleotide probe were used to specifically detect proteolytic and nonproteolytic Clostridium botulinum type B. Two synthetic primers deduced from the amino acid sequence data of type B neurotoxin were used to amplify a 1.5-kbp fragment corresponding to the li...

journal_title：Applied and environmental microbiology

authors： Szabo EA,Pemberton JM,Desmarchelier PM

更新日期：1992-01-01 00:00:00

abstract：:Decontamination treatments of burning and biocide application, alone and in combination with tillage, were evaluated for their ability to reduce populations of bacteria applied to the leaves of plants in field plots. In addition, the effects of these control methods on indigenous leaf and soil bacteria and fungi were ...

journal_title：Applied and environmental microbiology

authors： Donegan K,Fieland V,Fowles N,Ganio L,Seidler R

更新日期：1992-04-01 00:00:00

abstract：:The bacterium Rhodopseudomonas palustris grows with the aromatic acid benzoate and the alicyclic acid cyclohexanecarboxylate (CHC) as sole carbon sources. The enzymatic steps in an oxygen-independent pathway for CHC degradation have been elucidated, but it was unknown how the CHC operon (badHI aliAB badK) encoding the...

journal_title：Applied and environmental microbiology

authors： Hirakawa H,Hirakawa Y,Greenberg EP,Harwood CS

更新日期：2015-07-01 00:00:00

abstract：:Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...

journal_title：Applied and environmental microbiology

authors： Cleveland TE,Lax AR,Lee LS,Bhatnagar D

更新日期：1987-07-01 00:00:00

abstract：:A simple replica method is described for the rapid identification of colonies of bacteria which adhere to polystyrene. A correlation was found between the adherence of bacterial strains to polystyrene and cell surface hydrophobicity, suggesting the use of this technique in screening for cell surface mutants and in the...

journal_title：Applied and environmental microbiology

authors： Rosenberg M

更新日期：1981-08-01 00:00:00

abstract：:The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...

journal_title：Applied and environmental microbiology

authors： Dorella FA,Estevam EM,Pacheco LG,Guimarães CT,Lana UG,Gomes EA,Barsante MM,Oliveira SC,Meyer R,Miyoshi A,Azevedo V

更新日期：2006-11-01 00:00:00

abstract：:Saccharomyces cerevisiae killer strains secrete a protein toxin active on nonkiller strains of the same (or other) yeast species. Different killer toxins, K1, K2, K28, and Klus, have been described. Each toxin is encoded by a medium-size (1.5- to 2.3-kb) M double-stranded RNA (dsRNA) located in the cytoplasm. M dsRNAs...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Cousiño N,Esteban R

更新日期：2017-02-01 00:00:00

abstract：:Genes of Salmonella enterica serovar Typhimurium LT2 expected to be specifically present in Salmonella were selected using the Basic Local Alignment Search Tool (BLAST) program. The 152 selected genes were compared with 11 genomic sequences of Salmonella serovars, including Salmonella enterica subsp. I and IIIb and Sa...

journal_title：Applied and environmental microbiology

authors： Kim HJ,Park SH,Kim HY

更新日期：2006-09-01 00:00:00

abstract：:We investigated the effect of preincubation of environmental waters amended with inorganic nutrients (nitrogen, phosphorus, and traces of iron and magnesium) on the kinetics of the microbial transformation of phenol, propanil, propyl ester of (2,4-dichlorophenoxy)acetic acid, methyl parathion, Ronnel, and methoxychlor...

journal_title：Applied and environmental microbiology

authors： Paris DF,Rogers JE

更新日期：1986-02-01 00:00:00

abstract：:The single glucosyltransferase (GTF) of Streptococcus gordonii Challis CH1 makes alpha 1,3- and alpha 1,6-linked glucans from sucrose. The GTF carboxyl-terminal region has six direct repeats thought to be involved in glucan binding. Strains with defined mutations in this region have been described recently (M. M. Vick...

journal_title：Applied and environmental microbiology

authors： Vickerman MM,Clewell DB

更新日期：1997-05-01 00:00:00