Abstract:
:A simple replica method is described for the rapid identification of colonies of bacteria which adhere to polystyrene. A correlation was found between the adherence of bacterial strains to polystyrene and cell surface hydrophobicity, suggesting the use of this technique in screening for cell surface mutants and in the isolation of hydrophobic bacteria from nature.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Rosenberg Mdoi
10.1128/AEM.42.2.375-377.1981subject
Has Abstractpub_date
1981-08-01 00:00:00pages
375-7issue
2eissn
0099-2240issn
1098-5336journal_volume
42pub_type
杂志文章abstract::Bifidobacterium and Lactobacillus can beneficially affect the host by producing acetic acid and lactic acid, which lower pH and thereby inhibit the growth of pathogens or allow the probiotic bacteria to compete with pathogens for epithelial adhesion sites and nutrients. The transmural migration of enteric organisms in...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.05605-11
更新日期:2012-02-01 00:00:00
abstract::Pseudomonas aeruginosa is an opportunistic human pathogen and has been established as a model organism to study bacterial biofilm formation. At least three exopolysaccharides (alginate, Psl, and Pel) contribute to the formation of biofilms in this organism. Here mutants deficient in the production of one or more of th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00637-11
更新日期:2011-08-01 00:00:00
abstract::A simple synthetic liquid medium containing a single amino acid, glucose, salts, trace metals, and thiamine was developed for cultivation of Cercospora rosicola Passerini. Thiamine was shown to be important to growth. Culture of C. rosicola Passerini in a chemically defined medium makes possible studies of (+)-abscisi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.41.1.334-336.1981
更新日期:1981-01-01 00:00:00
abstract::To survive in various environments, from host tissue to soil, opportunistic bacterial pathogens must be metabolically flexible and able to use a variety of nutrient sources. We are interested in Pseudomonas aeruginosa's catabolism of quaternary amine compounds that are prevalent in association with eukaryotes. Carniti...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03943-12
更新日期:2013-06-01 00:00:00
abstract::This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.2.938-941.2002
更新日期:2002-02-01 00:00:00
abstract::The contribution of the biochemical pathways nitrification, denitrification, and dissimilatory NO3- reduction to NH4+ (DNRA) to the accumulation of NO2- in freshwaters is governed by the species compositions of the bacterial populations resident in the sediments, available carbon (C) and nitrogen (N) substrates, and e...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.1.61-66.1999
更新日期:1999-01-01 00:00:00
abstract::In this article, we present a new bioluminescent test system for the screening of chemical compounds with an inhibitory effect on protein synthesis. The test is based on the measurement of real-time in vivo light production by Escherichia coli strains expressing different luciferase genes. The eukaryotic lucGR gene fr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.8.2981-2989.1995
更新日期:1995-08-01 00:00:00
abstract::Inhibition of enzyme activity by high concentrations of substrate and/or cofactor is a general phenomenon demonstrated in many enzymes, including aldehyde dehydrogenases. Here we show that the uncharacterized protein BetB (SA2613) from Staphylococcus aureus is a highly specific betaine aldehyde dehydrogenase, which ex...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00215-14
更新日期:2014-07-01 00:00:00
abstract::The outer membrane of microbial cells forms an effective barrier for hydrophobic compounds, potentially causing an uptake limitation for hydrophobic substrates. Low bioconversion activities (1.9 U g(cdw)(-1)) have been observed for the ω-oxyfunctionalization of dodecanoic acid methyl ester by recombinant Escherichia c...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00949-12
更新日期:2012-08-01 00:00:00
abstract::The phylogenetic diversity of the ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBisCO, E.C. 4.1.1.39) large-subunit genes of deep-sea microorganisms was analyzed. Bulk genomic DNA was isolated from seven samples, including samples from the Mid-Atlantic Ridge and various deep-sea habitats around Japan. The kinds o...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.4.1751-1765.2001
更新日期:2001-04-01 00:00:00
abstract::An optical method to quantify the fungal hyphae within decomposing leaves of deciduous trees was developed. The plant matrix was partially destroyed under hydrolytic conditions, and fungal hyphae and cellulose residues within the leaves were stained with Calcofluor M2R. Cellulose residues were subsequently depolymeriz...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.11.3910-3918.1995
更新日期:1995-11-01 00:00:00
abstract::A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.8.3233-3241.1997
更新日期:1997-08-01 00:00:00
abstract::We present a phylogenetic analysis of nine strains of symbiotic nitrogen-fixing bacteria isolated from nodules of tagasaste (Chamaecytisus proliferus) and other endemic woody legumes of the Canary Islands, Spain. These and several reference strains were characterized genotypically at different levels of taxonomic reso...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.6.2096-2104.1998
更新日期:1998-06-01 00:00:00
abstract::Heterocystous filamentous cyanobacterium Anabaena cylindrica B629 and nonheterocystous filamentous cyanobacterium Oscillatoria sp. strain Miami BG7 were cultured in media with N(2) as the sole nitrogen source; and activities of oxygen-dependent hydrogen uptake, photohydrogen production, photooxygen evolution, and resp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.2.287-291.1985
更新日期:1985-08-01 00:00:00
abstract::A strain of the aerobic anoxygenic photosynthetic bacteria was isolated from a deep-ocean hydrothermal vent plume environment. The in vivo absorption spectra of cells indicate the presence of bacteriochlorophyll a incorporated into light-harvesting complex I and a reaction center. The general morphological and physiol...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.1.337-341.1998
更新日期:1998-01-01 00:00:00
abstract::Previously, we demonstrated conjugal transfer of a specially constructed shuttle vector, pRDB5, from the human colonic anaerobe Bacteroides uniformis to the ruminal anaerobe Prevotella (Bacteroides) ruminicola B(1)4. We have now shown that naturally occurring gene transfer elements in Bacteroides species and Prevotell...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.4.1313-1320.1992
更新日期:1992-04-01 00:00:00
abstract::The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.9.4163-4170.1999
更新日期:1999-09-01 00:00:00
abstract::Streptococcus oralis is the predominant aciduric nonmutans streptococcus isolated from the human dentition, but the role of this organism in the initiation and progression of dental caries has yet to be established. To identify proteins that are differentially expressed by S. oralis growing under conditions of low pH,...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.8.3396-3405.2001
更新日期:2001-08-01 00:00:00
abstract::A total of 896 samples of retail fresh meats and poultry was assayed for Escherichia coli serogroup O157:H7 by a hydrophobic grid membrane filter-immunoblot procedure developed specifically to isolate the organism from foods. The procedure involves several steps, including selective enrichment, filtration of enrichmen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.10.2394-2396.1987
更新日期:1987-10-01 00:00:00
abstract::Across evolution, N-glycosylation involves oligosaccharyltransferases that transfer lipid-linked glycans to selected Asn residues of target proteins. While these enzymes catalyze similar reactions in each domain, differences exist in terms of the chemical composition, length and degree of phosphorylation of the lipid ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03191-13
更新日期:2014-01-01 00:00:00
abstract::Rapid population growth and urban development along waterways and coastal areas have led to decreasing water quality. To examine the effects of upstream anthropogenic activities on microbiological water quality, methods for source-specific testing are required. In this study, molecular assays targeting human enterovir...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.4.2070-2078.2005
更新日期:2005-04-01 00:00:00
abstract::Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages pr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00067-13
更新日期:2013-05-01 00:00:00
abstract::Heavy metal resistance by bacteria is a topic of much importance to the bioremediation of contaminated soils and sediments. We report here the isolation of a highly cadmium-resistant Klebsiella planticola strain, Cd-1, from reducing salt marsh sediments. The strain grows in up to 15 mM CdCl(2) under a wide range of Na...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.3083-3087.2000
更新日期:2000-07-01 00:00:00
abstract::Lysostaphin represents a promising therapeutic agent for the treatment of staphylococcal infections, in particular those of methicillin-resistant Staphylococcus aureus (MRSA). However, conventional expression systems for the enzyme suffer from various limitations, and there remains a need for an efficient and cost-eff...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03914-13
更新日期:2014-05-01 00:00:00
abstract::We have assembled a collection of 13 psychrophilic ligA alleles that can serve as genetic elements for engineering mesophiles to a temperature-sensitive (TS) phenotype. When these ligA alleles were substituted into Francisella novicida, they conferred a TS phenotype with restrictive temperatures between 33 and 39°C. W...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03890-15
更新日期:2016-01-15 00:00:00
abstract::Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02178-16
更新日期:2016-11-21 00:00:00
abstract::Traditional three-domain fungal and bacterial laccases have been extensively studied for their significance in various biotechnological applications. Growing molecular evidence points to a wide occurrence of more recently recognized two-domain laccase-like multicopper oxidase (LMCO) genes in Streptomyces spp. However,...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00223-14
更新日期:2014-06-01 00:00:00
abstract::Since 2006, Geomyces destructans, the causative agent of white nose syndrome (WNS), has killed over 5.7 million bats in North America. The current hypothesis suggests that this novel fungus is an invasive species from Europe, but little is known about the diversity within the genus Geomyces and its distribution on bat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01429-13
更新日期:2013-09-01 00:00:00
abstract::The leaf colonization strategies of two bacterial strains were investigated. The foliar pathogen Pseudomonas syringae pv. syringae strain B728a and the nonpathogen Pantoea agglomerans strain BRT98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.2.1220-1228.2003
更新日期:2003-02-01 00:00:00
abstract::Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02555-20
更新日期:2021-01-29 00:00:00