Outer membrane protein AlkL boosts biocatalytic oxyfunctionalization of hydrophobic substrates in Escherichia coli.

abstract：:Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Covert S,Cullen D

更新日期：1993-10-01 00:00:00

abstract：:Of 34 strains of marine bacteria isolated on a general seawater medium, 5 were selected for detailed studies of their population dynamics in the plankton. The isolates were characterized as Aeromonas sp., Chromobacterium cf. lividum, Vibrio sp., and two Pseudomonas spp. Specific antibodies were produced by immunizatio...

journal_title：Applied and environmental microbiology

authors： Dahle AB,Laake M

更新日期：1982-01-01 00:00:00

abstract：:Downstream from the ptsHI operon of Lactobacillus sakei, the genes atkY and atkB, organized in an operon, were observed. The two putative proteins, AtkB and AtkY, show sequence similarity to the Enterococcus hirae copper P-type ATPase, responsible for copper efflux, and its negative regulator. Characterization of AtkB...

journal_title：Applied and environmental microbiology

authors： Stentz R,Loizel C,Malleret C,Zagorec M

更新日期：2000-10-01 00:00:00

abstract：:Fusarium fungal contaminants and related mycotoxins were investigated in eight maize feed samples submitted to the Iowa State University Veterinary Diagnostic Laboratory. Fusarium moniliforme, F. proliferatum, and F. subglutinans were isolated from seven, eight, and five samples, respectively. These strains belonged t...

journal_title：Applied and environmental microbiology

authors： Munkvold G,Stahr HM,Logrieco A,Moretti A,Ritieni A

更新日期：1998-10-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00

abstract：:The bacterial succession on the skin of broiler chicken carcasses stored at 2 degrees C was traced, and the ability of representative isolates to produce off-odors was determined by using sterile leg and breast muscle sections. Off-odors were identified by olfactory and chemical means. The inability of peptone-iron ag...

journal_title：Applied and environmental microbiology

authors： Daud HB,McMeekin TA,Thomas CJ

更新日期：1979-03-01 00:00:00

abstract：:The diversity of fungi in permanently frozen soil from northeastern Siberia was studied by culture-independent PCR amplification of diverse environmental 18S rRNA genes. Elaborate protocols to avoid contamination during drilling, sampling, and amplification were used. A broad diversity of eukaryotic DNA sequences that...

journal_title：Applied and environmental microbiology

authors： Lydolph MC,Jacobsen J,Arctander P,Gilbert MT,Gilichinsky DA,Hansen AJ,Willerslev E,Lange L

更新日期：2005-02-01 00:00:00

abstract：:Erwinia carotovora subsp. atroseptica, E. carotovora subsp. betavasculorum, and E. carotovora subsp. carotovora produce high levels of extracellular enzymes, such as pectate lyase (Pel), polygalacturonase (Peh), cellulase (Cel), and protease (Prt), and the quorum-sensing signal N-acyl-homoserine lactone (AHL) at 28 de...

journal_title：Applied and environmental microbiology

authors： Hasegawa H,Chatterjee A,Cui Y,Chatterjee AK

更新日期：2005-08-01 00:00:00

abstract：:Purified manganese peroxidase (MnP) from the white-rot basidiomycete Phlebia radiata was found to convert in vitro milled pine wood (MPW) suspended in an aqueous reaction solution containing Tween 20, Mn(2+), Mn-chelating organic acid (malonate), and a hydrogen peroxide-generating system (glucose-glucose oxidase). The...

journal_title：Applied and environmental microbiology

authors： Hofrichter M,Lundell T,Hatakka A

更新日期：2001-10-01 00:00:00

abstract：:Free-living soybean rhizobia and Bradyrhizobium spp. (lupine) have the ability to catabolize ethanol. Of the 30 strains of rhizobia examined, only the fast- and slow-growing soybean rhizobia and the slow-growing Bradyrhizobium sp. (lupine) were capable of using ethanol as a sole source of carbon and energy for growth....

journal_title：Applied and environmental microbiology

authors： Sadowsky MJ,Bohlool BB

更新日期：1986-10-01 00:00:00

abstract：:Forty-two Helicobacter isolates were isolated from swine feces in The Netherlands and Denmark. All 12 isolates sequenced (16S rRNA gene) formed a robust clade with Helicobacter canadensis ( approximately 99% similarity). Species-specific PCR indicated that all of the isolates were H. canadensis isolates. Although the ...

journal_title：Applied and environmental microbiology

authors： Inglis GD,McConville M,de Jong A

更新日期：2006-06-01 00:00:00

abstract：:Cyanobacterial glycogen-deficient mutants display impaired degradation of light-harvesting phycobilisomes under nitrogen-limiting growth conditions and secrete a suite of organic acids as a putative reductant-spilling mechanism. This genetic background, therefore, represents an important platform to better understand ...

journal_title：Applied and environmental microbiology

authors： Jackson SA,Eaton-Rye JJ,Bryant DA,Posewitz MC,Davies FK

更新日期：2015-09-01 00:00:00

abstract：:Phytoplankton replace phosphorus-containing lipids (P-lipids) with non-P analogues, boosting growth in P-limited oceans. In the model diatom Thalassiosira pseudonana, the substitution dynamics of lipid headgroups are well described, but those of the individual lipids, differing in fatty acid composition, are unknown. ...

journal_title：Applied and environmental microbiology

authors： Hunter JE,Brandsma J,Dymond MK,Koster G,Moore CM,Postle AD,Mills RA,Attard GS

更新日期：2018-03-01 00:00:00

abstract：:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

journal_title：Applied and environmental microbiology

authors： Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

更新日期：2000-09-01 00:00:00

abstract：:Nutritional and physical factors which influence the germination of akinetes of Nodularia spumigena (Cyanobacteriaceae) were examined. Low concentrations of phosphorus (<0.9 muM) were required for germination. Nitrate had no effect, but ammonia, at concentrations of >45 muM, inhibited germination. Salinities of >20 pe...

journal_title：Applied and environmental microbiology

authors： Huber AL

更新日期：1985-01-01 00:00:00

abstract：:Cytolysin and gelatinase are prominent pathogenicity determinants associated with highly virulent Enterococcus faecalis strains. In an effort to explore the expression profiles of these virulence traits in vivo, we have employed E. faecalis variants expressing the luxABCDE cassette under the control of either the P16S...

journal_title：Applied and environmental microbiology

authors： La Rosa SL,Casey PG,Hill C,Diep DB,Nes IF,Brede DA

更新日期：2013-07-01 00:00:00

abstract：:Conditions for the production of the trypsin inhibitor from Cephalosporium sp. KM 388 were investigated. Polypeptone-meat extract-glucose medium supported excellent production of the trypsin inhibitor. In this medium, polypeptone and meat extract were utilized both as carbon and nitrogen sources and as limiting substr...

journal_title：Applied and environmental microbiology

authors： Tsuchiya K,Kimura T

更新日期：1978-04-01 00:00:00

abstract：:Several formulations of brilliant green agar with an added H2S indicator were evaluated. Results were optimum with variations of a basic formula consisting of 40 g of tryptic soy agar (Difco), 8 g of lactose, 8 g of sucrose, 80 mg of phenol red, 1 g of sulfanilamide, 1.5 g of ferric ammonium citrate, 5 g of sodium thi...

journal_title：Applied and environmental microbiology

authors： Moats WA,Kinner JA

更新日期：1976-03-01 00:00:00

abstract：:Generic taxon-specific DNA probes that target an internal region of the gene (rbcL) encoding the large subunit of ribulose-1, 5-bisphosphate carboxylase/oxygenase (RubisCO) were developed for two groups of marine phytoplankton (diatoms and prymnesiophytes). The specificity and utility of the probes were evaluated in t...

journal_title：Applied and environmental microbiology

authors： Wyman M,Davies JT,Crawford DW,Purdie DA

更新日期：2000-06-01 00:00:00

abstract：:This work presents the characterization of an uptake hydrogenase from a marine filamentous nonheterocystous cyanobacterium, Lyngbya majuscula CCAP 1446/4. The structural genes encoding the uptake hydrogenase (hupSL) were isolated and characterized, and regulatory sequences were identified upstream of hupS. In silico a...

journal_title：Applied and environmental microbiology

authors： Leitão E,Oxelfelt F,Oliveira P,Moradas-Ferreira P,Tamagnini P

更新日期：2005-08-01 00:00:00

abstract：:The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...

journal_title：Applied and environmental microbiology

authors： Gill AO,Holley RA

更新日期：2004-10-01 00:00:00

abstract：:DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...

journal_title：Applied and environmental microbiology

authors： Huys G,D'Haene K,Van Eldere J,von Holy A,Swings J

更新日期：2005-01-01 00:00:00

abstract：:Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

journal_title：Applied and environmental microbiology

authors： Nielsen KM,Bones AM,Van Elsas JD

更新日期：1997-10-01 00:00:00

abstract：:Cryparin is a cell-surface-associated hydrophobin of the filamentous ascomycete Cryphonectria parasitica. This protein contains a signal peptide that directs it to the vesicle-mediated secretory pathway. We detected a glycosylated form of cryparin in a secretory vesicle fraction, but secreted forms of this protein are...

journal_title：Applied and environmental microbiology

authors： McCabe PM,Van Alfen NK

更新日期：1999-12-01 00:00:00

abstract：:The objective of this study was to evaluate the effect of chitosan microparticles on the uterine microbiome of cows with metritis. Dairy cows with metritis (n = 89) were assigned to 1 of 3 treatments: chitosan microparticles (n = 21), in which the cows received an intrauterine infusion of chitosan microparticles at me...

journal_title：Applied and environmental microbiology

authors： Galvão KN,de Oliveira EB,Cunha F,Daetz R,Jones K,Ma Z,Jeong KC,Bicalho RC,Higgins CH,Rodrigues MX,Gonzalez Moreno C,Jeon S

更新日期：2020-09-01 00:00:00

abstract：:We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron don...

journal_title：Applied and environmental microbiology

authors： Jiao Y,Kappler A,Croal LR,Newman DK

更新日期：2005-08-01 00:00:00

abstract：:Pseudomonas fluorescens 2-79 suppresses take-all, a major root disease of wheat caused by Gaeumannomyces graminis var. tritici. The bacteria produce an antibiotic, phenazine-1-carboxylic acid (PCA), and a fluorescent pyoverdin siderophore. Previous studies have established that PCA has an important role in the biologi...

journal_title：Applied and environmental microbiology

authors： Hamdan H,Weller DM,Thomashow LS

更新日期：1991-11-01 00:00:00

abstract：:An outstanding challenge toward efficient production of biofuels and value-added chemicals from plant biomass is the impact that lignocellulose-derived inhibitors have on microbial fermentations. Elucidating the mechanisms that underlie their toxicity is critical for developing strategies to overcome them. Here, using...

journal_title：Applied and environmental microbiology

authors： Pisithkul T,Jacobson TB,O'Brien TJ,Stevenson DM,Amador-Noguez D

更新日期：2015-09-01 00:00:00

abstract：:A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

journal_title：Applied and environmental microbiology

authors： Jacobsen CS,Rasmussen OF

更新日期：1992-08-01 00:00:00

abstract：:A combination of inhibitors and carbon substrates was used to determine the relative contribution of sulfate-reducing bacteria (SRB) and fermenting bacteria to nitrogen fixation in a salt marsh sediment and on the roots of Spartina alterniflora. Because a lag period precedes acetylene-reducing activity (ARA) in amende...

journal_title：Applied and environmental microbiology

authors： Gandy EL,Yoch DC

更新日期：1988-08-01 00:00:00