Abstract:
:We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron donor and photoheterotrophically with a variety of organic carbon sources. TIE-1 also grows chemoheterotrophically in the dark. This isolate appears to be a new strain of the purple nonsulfur bacterial species Rhodopseudomonas palustris, based on physiological and phylogenetic analysis. Fe(II) oxidation is optimal at pH 6.5 to 6.9. The mineral products of Fe(II) oxidation are pH dependent: below pH 7.0 goethite (alpha-FeOOH) forms, and above pH 7.2 magnetite (Fe3O4) forms. TIE-1 forms colonies on agar plates and is sensitive to a variety of antibiotics. A hyperactive mariner transposon is capable of random insertion into the chromosome with a transposition frequency of approximately 10(-5). To identify components involved in phototrophic Fe(II) oxidation, mutants of TIE-1 were generated by transposon mutagenesis and screened for defects in Fe(II) oxidation in a cell suspension assay. Among approximately 12,000 mutants screened, 6 were identified that are specifically impaired in Fe(II) oxidation. Five of these mutants have independent disruptions in a gene that is predicted to encode an integral membrane protein that appears to be part of an ABC transport system; the sixth mutant has an insertion in a gene that is a homolog of CobS, an enzyme involved in cobalamin (vitamin B12) biosynthesis.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Jiao Y,Kappler A,Croal LR,Newman DKdoi
10.1128/AEM.71.8.4487-4496.2005keywords:
subject
Has Abstractpub_date
2005-08-01 00:00:00pages
4487-96issue
8eissn
0099-2240issn
1098-5336pii
71/8/4487journal_volume
71pub_type
杂志文章abstract::One of soil microbiology's most intriguing puzzles is how so many different bacterial species can coexist in small volumes of soil when competition theory predicts that less competitive species should decline and eventually disappear. We provide evidence supporting the theory that low pore connectivity caused by low w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03085-09
更新日期:2010-06-01 00:00:00
abstract::The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02072-07
更新日期:2008-06-01 00:00:00
abstract::A methanotroph (strain 68-1), originally isolated from a trichloroethylene (TCE)-contaminated aquifer, was identified as the type I methanotroph Methylomonas methanica on the basis of intracytoplasmic membrane ultrastructure, phospholipid fatty acid profile, and 16S rRNA signature probe hybridization. Strain 68-1 was ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.4.960-967.1993
更新日期:1993-04-01 00:00:00
abstract::Norovirus is one of the most common causes of acute viral gastroenteritis. The virus is spread via the fecal-oral route, most commonly from infected food and water, but several outbreaks have originated from contamination of surfaces with infectious virus. In this study, a close surrogate of human norovirus causing ga...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03280-14
更新日期:2015-02-01 00:00:00
abstract::Chrysophanol and islandicin, two anthraquinones which are structurally related to emodin, were found to be frame-shift mutagens for Salmonella typhimurium strain TA 1537 after metabolic activation. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.40.3.476-479.1980
更新日期:1980-09-01 00:00:00
abstract::Cyanobacteria can synthesize alkanes and alkenes, which are considered to be infrastructure-compatible biofuels. In terms of physiological function, cyanobacterial hydrocarbons are thought to be essential for membrane flexibility for cell division, size, and growth. The genetic basis for the biosynthesis of terminal o...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00425-18
更新日期:2018-06-18 00:00:00
abstract::The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or 37 degrees C), pH (5.5 or 7.4), the presence of salt and organic acids (375 mM NaCl, 8.45 mM sodium diacetate [SD], 275 mM...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00764-06
更新日期:2006-08-01 00:00:00
abstract::A method is described for generating and screening variants of the beta-galactosidase from Lactobacillus delbrueckii subsp. bulgaricus sensitive to several environmental stresses, with potential application in the food industry. Chemical mutagenesis with hydroxylamine or methoxylamine was performed on the beta-galacto...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.4.1221-1226.1994
更新日期:1994-04-01 00:00:00
abstract::Chlorpromazine was used to perform a test for the detection of microbial peroxidase activities. The compound acts as both a cell permeabilizer and a reagent in the procedure developed which allows the detection of peroxidase and peroxidase like reactions both semiquantitatively in whole cell determinations and quantit...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.6.1433-1435.1986
更新日期:1986-12-01 00:00:00
abstract::Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.3.475-477.1985
更新日期:1985-03-01 00:00:00
abstract::We investigated the prevalence of sapoviruses (SaVs) in the Tamagawa River in Japan from April 2003 to March 2004 and performed genetic analysis of the SaV genes identified in river water. A total of 60 river water samples were collected from five sites along the river, and 500 ml was concentrated using the cation-coa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02739-09
更新日期:2010-04-01 00:00:00
abstract::Potato tuber disks were submerged in suspensions containing 10 to 10 cells of Agrobacterium tumefaciens B6 per ml. After 60 min, the disks were rinsed and homogenized, and portions of the homogenates were plated to measure the number of adsorbed bacteria. At low initial bacterial concentrations (10/ml), 5 to 23% of th...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.6.1351-1355.1985
更新日期:1985-06-01 00:00:00
abstract::The size frequency distribution of planktonic cells of purple sulfur phototrophic bacteria was measured at several depths in a bacterial layer of Lake Cisó (Spain). The bacterioplankton was dominated by Chromatium minus (87 to 94% of the total biomass). The largest cells of C. minus were found in the top part of the b...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.53.4.864-871.1987
更新日期:1987-04-01 00:00:00
abstract::Cyclic lipopeptides (CLPs) are produced by many Pseudomonas species and have several biological functions, including a role in surface motility, biofilm formation, virulence, and antimicrobial activity. This study focused on the diversity and role of LuxR-type transcriptional regulators in CLP biosynthesis in Pseudomo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00575-09
更新日期:2009-07-01 00:00:00
abstract::beta-d-Mannanase from Bacillus pumilus was purified into two components, A and B, which exhibited homogeneity on polyacrylamide gel electrophoresis. These components had molecular weights of 55,000 (A) and 37,000 (B); carbohydrate contents of 15.3% (A) and 7.2% (B); specific activities of 78 (A) and 1,616 (B) U/mg; pH...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.6.1954-1956.1990
更新日期:1990-06-01 00:00:00
abstract::Bacillus thuringiensis serovar israelensis (B. thuringiensis subsp. israelensis) produces four insecticidal crystal proteins (ICPs) (Cry4A, Cry4B, Cry11A, and Cyt1A). Toxicity of recombinant B. thuringiensis subsp. israelensis strains expressing only one of the toxins was determined with first instars of Tipula paludo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01056-06
更新日期:2007-06-01 00:00:00
abstract::Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. However, little is known about the genes and factors involved in its basic physiology and virulence properties. Insertion mutagenesis was used to initiate the identification and characterization of some...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.12.6353-6360.2002
更新日期:2002-12-01 00:00:00
abstract::Techniques and media for the production and regeneration of stable Clostridium acetobutylicum protoplasts are described. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.3.719-721.1982
更新日期:1982-03-01 00:00:00
abstract::The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. The concentrations of nitrate and uranium were in the ranges of 0.1 ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00917-15
更新日期:2015-08-01 00:00:00
abstract::The degradation of the herbicides EPTC (S-ethyl dipropylthiocarbamate) and atrazine (2-chloro-4-ethyl-amino-6-isopropylamino-1,3,5-triazine) is associated with an indigenous plasmid in Rhodococcus sp. strain TE1. Plasmid DNA libraries of Rhodococcus sp. strain TE1 were constructed in a Rhodococcus-Escherichia coli shu...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.5.2061-2065.1995
更新日期:1995-05-01 00:00:00
abstract::A method is described whereby the presence of Salmonella spp. can be detected within 8 to 24 h of sample collection. The method depends upon the interaction of Salmonella spp. with the Salmonella-specific Felix-O1 bacteriophage. This interaction results in an increase in concentration of the bacteriophage which is det...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.1.260-264.1983
更新日期:1983-01-01 00:00:00
abstract::Bacterial strains were isolated on the basis of their ability to proliferate in a minimal medium containing one of a series of lignin-related compounds as the sole carbon and energy source. These included the aromatic monomers guaiacol, vanillic and coumaric acids, a dimer and a trimer possessing the arylglycerol-beta...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.6.1428-1432.1986
更新日期:1986-12-01 00:00:00
abstract::The effect of yeast hulls (yeast ghosts) on sluggish or stuck white wine fermentations was studied. The enhancing effect on yeast growth and fermentation rate displayed by the hulls was shown to be similar to the effect provided by lipid extract from the same hulls. Unsaturated fatty acids and sterols were incorporate...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.6.1560-1564.1989
更新日期:1989-06-01 00:00:00
abstract::Cytolethal distending toxins (CDT) are potent cytotoxins of several Gram-negative pathogenic bacteria, including Escherichia coli, in which five types (CDT-I to CDT-V) have been identified so far. CDT-V is frequently associated with Shiga-toxigenic E. coli (STEC), enterohemorrhagic E. coli (EHEC) O157 strains, and str...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01134-13
更新日期:2013-08-01 00:00:00
abstract::The present work describes the development of a superior strain of Corynebacterium glutamicum for diaminopentane (cadaverine) production aimed at the identification and deletion of the underlying unknown N-acetyldiaminopentane pathway. This acetylated product variant, recently discovered, is a highly undesired by-prod...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00834-10
更新日期:2010-08-01 00:00:00
abstract::Dibenzothiophene (DBT) and its derivatives can be microbially desulfurized by enzymes DszC, DszA, and DszB, which are encoded by the operon dszABC and contribute to the conversion in tandem. We investigated the expression characteristics of the dsz operon. Our results revealed that the levels of transcription and tran...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02319-07
更新日期:2008-02-01 00:00:00
abstract::Pervasive environmental stressors on coral reefs are attributed with shifting the competitive balance in favor of alternative dominants, such as macroalgae. Previous studies have demonstrated that macroalgae compete with corals via a number of mechanisms, including the production of potent primary and secondary metabo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02391-16
更新日期:2016-12-15 00:00:00
abstract::The plasmid profiles of 619 cultures of Bacillus anthracis which had been isolated and stored between 1954 and 1989 were analyzed using the Laboratory Response Network real-time PCR assay targeting a chromosomal marker and both virulence plasmids (pXO1 and pXO2). The cultures were stored at ambient temperature on tryp...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.12.7778-7780.2005
更新日期:2005-12-01 00:00:00
abstract::Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and n...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.7.2228-2235.1996
更新日期:1996-07-01 00:00:00
abstract::Virus adsorption to soils is considered to be the most important factor in removing viruses after land treatment of wastewater. Most of the studies on virus adsorption to soils have utilized poliovirus as the model system. In the present study, comparative adsorption of a number of different types and strains of human...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.38.2.241-247.1979
更新日期:1979-08-01 00:00:00