Isotherm for Adsorption of Agrobacterium tumefaciens to Susceptible Potato (Solanum tuberosum L.) Tissues.

Abstract:

:Potato tuber disks were submerged in suspensions containing 10 to 10 cells of Agrobacterium tumefaciens B6 per ml. After 60 min, the disks were rinsed and homogenized, and portions of the homogenates were plated to measure the number of adsorbed bacteria. At low initial bacterial concentrations (10/ml), 5 to 23% of the bacteria adsorbed. At higher bacterial concentrations, the corresponding value was approximately 1.2%. Adsorption was a reversible equilibrium process. Binding saturation was not achieved, and adsorbed bacteria were confined to monolayers on the surfaces of tissue prepared for scanning electron microscopy. Adsorption of strain B6 to potato tuber tissues is described accurately by the Freundlich adsorption isotherm and may be a nonspecific phenomenon.

journal_name

Appl Environ Microbiol

authors

Kluepfel DA,Pueppke SG

doi

10.1128/AEM.49.6.1351-1355.1985

keywords:

subject

Has Abstract

pub_date

1985-06-01 00:00:00

pages

1351-5

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

49

pub_type

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